Ascidian eggs block polyspermy by two independent mechanisms: one at the egg plasma membrane, the other involving the follicle cells.

abstract：:Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...

journal_title：Molecular reproduction and development

authors： Reyes-Moreno C,Laflamme J,Frenette G,Sirard MA,Sullivan R

更新日期：2008-03-01 00:00:00

abstract：:A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...

journal_title：Molecular reproduction and development

authors： Mathialagan N,Bixby JA,Roberts RM

更新日期：1992-08-01 00:00:00

abstract：:Regulation of protein tyrosine phosphorylation is required for sperm capacitation and oocyte fertilization. The objective of the present work was to study the role of the calcium-sensing receptor (CaSR) on protein tyrosine phosphorylation in boar spermatozoa under capacitating conditions. To do this, boar spermatozoa ...

journal_title：Molecular reproduction and development

authors： Macías-García B,García-Marín LJ,Bragado MJ,González-Fernández L

更新日期：2019-07-01 00:00:00

abstract：:Human endometrial epithelium (EE) is composed of a multitude of proteins, amongst which those localized on the plasma membrane [plasma membrane proteins (PMPs)] are of critical relevance in the early stages of implantation. Evidence supports the key role of few PMPs in implantation. However, many remain unidentified, ...

journal_title：Molecular reproduction and development

authors： Kakar-Bhanot R,Brahmbhatt K,Kumar V,Suryawanshi AR,Srivastava S,Chaudhari U,Sachdeva G

更新日期：2020-01-01 00:00:00

abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

journal_title：Molecular reproduction and development

authors： Martin RH,Chernos JE,Rademaker AW

更新日期：1991-10-01 00:00:00

abstract：:We determined the incidence of activation, male pronuclear formation, and apposition of pronuclei in porcine oocytes following intracytoplasmic injection of various porcine sperm components and foreign species spermatozoa, such as that of cattle, mouse or human. The porcine oocytes were activated by injection of a spe...

journal_title：Molecular reproduction and development

authors： Kim NH,Jun SH,Do JT,Uhm SJ,Lee HT,Chung KS

更新日期：1999-05-01 00:00:00

abstract：:RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by comm...

journal_title：Molecular reproduction and development

authors： Sugiura K,Su YQ,Eppig JJ

更新日期：2009-06-01 00:00:00

abstract：:During the molecular cloning of the ovine testicular follicle-stimulating (FSH) receptor that couples to the Gs-type effector systems, we discovered novel cDNA clones that were highly homologous. Some of these clones contained an insert of 1,584 bp, which consisted of a divergent 3' region spliced with a 5' region tha...

journal_title：Molecular reproduction and development

authors： Yarney TA,Jiang L,Khan H,MacDonald EA,Laird DW,Sairam MR

更新日期：1997-12-01 00:00:00

abstract：:Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin i...

journal_title：Molecular reproduction and development

authors： Aoki Y,Tsujimura A,Kaseda K,Okabe M,Tokuhiro K,Ohta T,O'Bryan MK,Okuda H,Kitamura K,Ogawa Y,Fujiki T,Wada M,Horie S,Nishimune Y,Tanaka H

更新日期：2020-05-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...

journal_title：Molecular reproduction and development

authors： Murray MK,DeSouza MM

更新日期：1995-11-01 00:00:00

abstract：:Among Sicilian stick insects, two hybridogenetic complexes have been discovered: Bacillus rossius-grandii benazzii and B. rossius-grandii grandii, which also produce androgenetic offspring. The egg maturation of the former is analyzed here through DAPI fluorometry, which, besides the assessment of the meiotic stages, ...

journal_title：Molecular reproduction and development

authors： Tinti F,Scali V

更新日期：1992-11-01 00:00:00

abstract：:Teleost fish exhibit remarkably diverse and plastic patterns of sexual development. One of the most fascinating modes of plasticity is functional sex change, which is widespread in marine fish including species of commercial importance; however, the regulatory mechanisms remain elusive. In this review, we explore such...

journal_title：Molecular reproduction and development

authors： Liu H,Todd EV,Lokman PM,Lamm MS,Godwin JR,Gemmell NJ

更新日期：2017-02-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...

journal_title：Molecular reproduction and development

authors： Ellison AR,Wallace H,al-Shawi R,Bishop JO

更新日期：1995-08-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...

journal_title：Molecular reproduction and development

authors： Silvestre F,Boni R,Fissore RA,Tosti E

更新日期：2011-10-01 00:00:00

abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

journal_title：Molecular reproduction and development

authors： Xiong W,Wang Z,Shen C

更新日期：2019-08-01 00:00:00

abstract：:Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...

journal_title：Molecular reproduction and development

authors： Ergenc AF,Li MW,Toner M,Biggers JD,Lloyd KC,Olgac N

更新日期：2008-12-01 00:00:00

abstract：:Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL,Gil M,Rivkin E,Tres LL

更新日期：2002-09-01 00:00:00

abstract：:Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC...

journal_title：Molecular reproduction and development

authors： Fuku EJ,Liu J,Downey BR

更新日期：1995-02-01 00:00:00

abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

journal_title：Molecular reproduction and development

authors： Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

更新日期：2007-09-01 00:00:00

abstract：:The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium-sized follicles (3-8 mm in diameter) were untreated (MCO) or treated with ...

journal_title：Molecular reproduction and development

authors： You J,Kim J,Lee H,Hyun SH,Hansen PJ,Lee E

更新日期：2012-01-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...

journal_title：Molecular reproduction and development

authors： Adham IM,Nayernia K,Engel W

更新日期：1997-03-01 00:00:00

abstract：:Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...

journal_title：Molecular reproduction and development

authors： Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KK

更新日期：2017-03-01 00:00:00

abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...

journal_title：Molecular reproduction and development

authors： Munsie M,Schlatt S,deKretser DM,Loveland KL

更新日期：1997-05-01 00:00:00

abstract：:Pigs have a unique, non-invasive epitheliochorial placenta where maternal and fetal layers lay in apposition. Indentation of fetal capillaries into the trophoblasts and maternal capillaries into the uterine epithelium reduce the distance between the fetal and maternal blood, ensuring nutrient transfer for proper conce...

journal_title：Molecular reproduction and development

authors： Bidarimath M,Tayade C

更新日期：2017-09-01 00:00:00

abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...

journal_title：Molecular reproduction and development

authors： Chen X,Yue Y,He Y,Zhu H,Hao H,Zhao X,Qin T,Wang D

更新日期：2014-10-01 00:00:00