CNP/NPR2 signaling maintains oocyte meiotic arrest in early antral follicles and is suppressed by EGFR-mediated signaling in preovulatory follicles.

abstract：:The objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum-fed (SF), serum-starved (SS), and 1, or 5 microM 5-azacytidine (5-aza-CR) treated cells was digested with a methylation sensitive e...

journal_title：Molecular reproduction and development

authors： Jones KL,Hill J,Shin TY,Lui L,Westhusin M

更新日期：2001-10-01 00:00:00

abstract：:Three monoclonal antibodies against human protamines were obtained by immunization with total human basic nuclear proteins or purified protamine HP3. The specificity of antibodies was assessed by enzyme-linked immunosorbent assay (ELISA) and Western blot. They recognized three distinct epitopes: One was specific for t...

journal_title：Molecular reproduction and development

authors： Le Lannic G,Arkhis A,Vendrely E,Chevaillier P,Dadoune JP

更新日期：1993-09-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC...

journal_title：Molecular reproduction and development

authors： Fuku EJ,Liu J,Downey BR

更新日期：1995-02-01 00:00:00

abstract：:Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...

journal_title：Molecular reproduction and development

authors： van Engelen E,Breeveld-Dwarkasing VN,Taverne MA,Everts ME,van der Weijden GC,Rutten VP

更新日期：2008-11-01 00:00:00

abstract：:Unlike in higher vertebrates, in fish it is not known whether the nerve supply of the testis can influence testicular functions or not. In addition to neurotransmitters, nerve terminals may release ATP and adenosine in the extracellular medium. On the assumption that these molecules might be released by fibers innerva...

journal_title：Molecular reproduction and development

authors： Loir M

更新日期：1999-08-01 00:00:00

abstract：:This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...

journal_title：Molecular reproduction and development

authors： Downs SM,Chen J

更新日期：2008-01-01 00:00:00

abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...

journal_title：Molecular reproduction and development

authors： Moreau R,Daoud G,Masse A,Simoneau L,Lafond J

更新日期：2003-07-01 00:00:00

abstract：:A mouse cDNA encoding a putative DNA-binding protein of the zinc-finger type was isolated from an E8.5 mouse embryonic cDNA library. Sequence comparison revealed a high degree of homology between this mouse cDNA and the human and rat orphan receptor Tr2-11 isolated from prostate cDNA libraries. This transcript was det...

journal_title：Molecular reproduction and development

authors： Lee CH,Chang L,Wei LN

更新日期：1996-07-01 00:00:00

abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...

journal_title：Molecular reproduction and development

authors： Sakakibara R,Sakai K,Sakurai Y,Kohnoura T,Ishiguro M

更新日期：1993-01-01 00:00:00

abstract：:The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

journal_title：Molecular reproduction and development

authors： Manning KR

更新日期：2009-10-01 00:00:00

abstract：:Prolactin-like protein C (PLP-C) is a major rat placental protein which is expressed during the second half of pregnancy and belongs to the growth hormone-prolactin family. Here we report on the isolation of overlapping rat placental cDNAs which specify a transcript of 915 base pairs and predict a 205-amino acid trans...

journal_title：Molecular reproduction and development

authors： Conliffe PR,Simmen RC,Buhi WC,Shiverick KT

更新日期：1995-06-01 00:00:00

abstract：:In vitro studies have suggested that the Cables1 gene regulates epithelial cell proliferation, whereas other studies suggest a role in promoting neural differentiation. In efforts to clarify the functions of Cables1 in vivo, we conducted gain- and loss-of-function studies targeting its ortholog (cables1) in the zebraf...

journal_title：Molecular reproduction and development

authors： Groeneweg JW,White YA,Kokel D,Peterson RT,Zukerberg LR,Berin I,Rueda BR,Wood AW

更新日期：2011-01-01 00:00:00

abstract：:Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...

journal_title：Molecular reproduction and development

authors： Sim BW,Cha JJ,Song BS,Kim JS,Yoon SB,Choi SA,Jeong KJ,Kim YH,Huh JW,Lee SR,Kim SH,Lee CS,Kim SU,Chang KT

更新日期：2013-03-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...

journal_title：Molecular reproduction and development

authors： Bajpai R,Lesperance J,Kim M,Terskikh AV

更新日期：2008-05-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...

journal_title：Molecular reproduction and development

authors： Harris MS,Rodger JC

更新日期：1998-08-01 00:00:00

abstract：:The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...

journal_title：Molecular reproduction and development

authors： Hyttel P,Vajta G,Callesen H

更新日期：2000-05-01 00:00:00

abstract：:In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...

journal_title：Molecular reproduction and development

authors： Gu W,Kwon YK,Hecht NB

更新日期：1996-06-01 00:00:00

abstract：:Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...

journal_title：Molecular reproduction and development

authors： Martínez ML,Martelotto L,Cabada MO

更新日期：2000-10-01 00:00:00

abstract：:Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...

journal_title：Molecular reproduction and development

authors： Xiao LJ,Chang H,Ding NZ,Ni H,Kadomatsu K,Yang ZM

更新日期：2002-09-01 00:00:00

abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...

journal_title：Molecular reproduction and development

authors： Foster JA,Gerton GL

更新日期：1996-06-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Biochemical and immunoelectron microscopic methods have been used to analyze the distribution of actin in boar spermatozoa and its state of aggregation before and after acrosome reaction. F-actin was detected on sperm head and tail by electron microscopy using an improved phalloidin probe: incubation with a fluorescei...

journal_title：Molecular reproduction and development

authors： Castellani-Ceresa L,Brivio MF,Radaelli G

更新日期：1992-09-01 00:00:00

abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...

journal_title：Molecular reproduction and development

authors： Benson S,Rawson R,Killian C,Wilt F

更新日期：1991-07-01 00:00:00

abstract：:Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and ...

journal_title：Molecular reproduction and development

authors： Garside WT,Loret de Mola JR,Bucci JA,Tureck RW,Heyner S

更新日期：1997-05-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...

journal_title：Molecular reproduction and development

authors： Tourte M,Besse C,Mounolou JC

更新日期：1991-12-01 00:00:00

abstract：:Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature ...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Zhang D,Pan Q,Ding M,Deng H

更新日期：2006-10-01 00:00:00

abstract：:Treatment of in vitro matured bovine oocytes with colcemid results in a membrane protrusion that contains maternal chromosomes, which can be easily removed by aspiration. Four experiments were designed to evaluate the overall and temporal effects of conditioned medium (CM) by bovine cumulus cells on development of nuc...

journal_title：Molecular reproduction and development

authors： Li GP,Bunch TD,White KL,Aston KI,Meerdo LN,Pate BJ,Sessions BR

更新日期：2004-06-01 00:00:00