Abstract:
:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA bound to NLS or nuclear import deficient reverse NLS (revNLS) peptides. After 3 hr, DNA-NLS, but not DNA-revNLS, complexes were bound around the nuclear periphery. We demonstrate that nuclear import of DNA-NLS complexes is a two-step process involving binding to, and translocation across, the nuclear envelope. Binding is ATP-independent, occurs at 0 degree C and is Ca(2+)-independent. By contrast, translocation requires ATP hydrolysis, Ca2+, is temperature dependent and is blocked by the lectin wheat germ agglutinin. Both binding and translocation are competitively inhibited by albumin-NLS conjugates, require heat-labile cytosolic factors, and are inhibited by N-ethylmaleimide treatment of the cytosol. Binding and translocation are differentially affected by cytosol dilutions, suggesting that at least two distinct soluble fractions are required for nuclear import. The requirements for NLS-mediated nuclear import of plasmid DNA are similar to those for nuclear import of protein-NLS conjugates in permeabilized cells.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Collas P,Aleström Pdoi
10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>subject
Has Abstractpub_date
1996-12-01 00:00:00pages
431-8issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>journal_volume
45pub_type
杂志文章abstract::Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10015
更新日期:2002-03-01 00:00:00
abstract::Human ejaculate spermatozoa contain multiple mRNA species carried over from earlier stages in spermatogenesis. To date, gene-specific RT-PCR or in situ hybridization has detected transcripts for beta-actin, heat shock proteins (HSP) 70 and 90, protamines (PRM) 1 and 2, transition protein (TNp) 2, HLA II, beta-integrin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2+<259::AID-MRD1
更新日期:2000-06-01 00:00:00
abstract::Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21365
更新日期:2011-09-01 00:00:00
abstract::Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23139
更新日期:2019-06-01 00:00:00
abstract::The cumulus cells that surround the mammalian oocyte become dispersed following the preovulatory surge of the pituitary gonadotropin, luteinizing hormone (LH). We have examined cumulus-oocyte complexes of PMSG-primed immature rats before and at 1, 2, 3, 4, 6, and 8 hr after injection of human chorionic gonadotropin (h...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280313
更新日期:1991-03-01 00:00:00
abstract::Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400316
更新日期:1995-03-01 00:00:00
abstract::The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23092
更新日期:2019-02-01 00:00:00
abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10072
更新日期:2002-05-01 00:00:00
abstract::Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22309
更新日期:2014-05-01 00:00:00
abstract::A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20104
更新日期:2004-08-01 00:00:00
abstract::Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21224
更新日期:2010-09-01 00:00:00
abstract::Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<448::AID-MRD12
更新日期:1997-08-01 00:00:00
abstract::The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3
更新日期:2000-01-01 00:00:00
abstract::Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10293
更新日期:2003-05-01 00:00:00
abstract::In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199906)53:2<127::AID-MRD1>
更新日期:1999-06-01 00:00:00
abstract::Using a specific protein binding assay we have shown that a spermatozoon of the ascidian Ciona intestinalis contains 1.58 +/- 0.74 x 10(-19) moles of inositol 1,4,5-tri-phosphate (InsP3), while a human spermatozoon contains 6.4 +/- 0.14 x 10(-19) moles. Induction of the acrosome reaction (AR) in both species, by expos...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350109
更新日期:1993-05-01 00:00:00
abstract::Haprin (TRIM36) is a ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is expressed in the testes in both mice and humans and is thought to be involved in spermiogenesis, the acrosome reaction, and fertilization. However, the functional role of Haprin i...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23344
更新日期:2020-05-01 00:00:00
abstract::In vitro studies have suggested that the Cables1 gene regulates epithelial cell proliferation, whereas other studies suggest a role in promoting neural differentiation. In efforts to clarify the functions of Cables1 in vivo, we conducted gain- and loss-of-function studies targeting its ortholog (cables1) in the zebraf...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21263
更新日期:2011-01-01 00:00:00
abstract::EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22119
更新日期:2012-12-01 00:00:00
abstract::The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<323::AID-MRD3>3.0.CO
更新日期:2000-12-01 00:00:00
abstract::This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20781
更新日期:2008-01-01 00:00:00
abstract::The timing of the transition from maternal to zygotic control of development (MZT) and the initiation of transcription was studied in domestic cat embryos to determine if there is a temporal association between these phenomena and the development block observed in cat embryos fertilized in vitro. Embryos were derived ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199710)48:2<208::AID-MRD8>
更新日期:1997-10-01 00:00:00
abstract::It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10208
更新日期:2003-02-01 00:00:00
abstract::To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080010108
更新日期:1988-01-01 00:00:00
abstract::Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(20010401)58:4<432::AID-MRD11>3.0
更新日期:2001-04-01 00:00:00
abstract::Mouse zygotes or halves of zygotes, containing either a female or a male pronucleus, were fused with ovulated metaphase II oocytes. In 59.7% of the resulting hybrid cells, the pronuclei underwent premature chromosome condensation (PCC). In some of these heterokaryons the 2 pronuclei differed in the dynamics of condens...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340112
更新日期:1993-01-01 00:00:00
abstract::The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.1080320207
更新日期:1992-06-01 00:00:00
abstract::Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22297
更新日期:2014-03-01 00:00:00
abstract::Sex-related growth rate differences in preimplantation mouse embryos were investigated. In experiment I, Day 3 embryos were recovered from reproductive tracts, classified according to developmental stage, and cultured for 24 hr in CZB medium containing glucose. Each embryo was then reclassified and stained for measure...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400108
更新日期:1995-01-01 00:00:00
abstract::Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410415
更新日期:1995-08-01 00:00:00