Abstract:
:Mouse zygotes or halves of zygotes, containing either a female or a male pronucleus, were fused with ovulated metaphase II oocytes. In 59.7% of the resulting hybrid cells, the pronuclei underwent premature chromosome condensation (PCC). In some of these heterokaryons the 2 pronuclei differed in the dynamics of condensation. Detectability of differential PCC of pronuclei (dPCC) depended on the type of preparation. In hybrids with PCC, produced by fusion of intact zygotes with metaphase II oocytes and processed for whole-mount preparations, one pronucleus was more advanced in the condensation process in 47% of cases. In air-dried preparations dPCC was detected in as many as 94% of hybrids. Experiments with the fusion of halves of zygotes with metaphase II oocytes have shown that the differential reaction of pronuclei to condensation factor depended on their parental origin. Maternal chromatin responded faster to the condensation factor and attained more advanced stages of PCC than paternal chromatin. Different responses of the maternal and paternal pronucleus to the condensation factor suggests that the 2 pronuclei are not identical with regard to the organization of chromatin and/or the lamin composition of the nuclear envelope.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Ciemerych MA,Czołowska Rdoi
10.1002/mrd.1080340112subject
Has Abstractpub_date
1993-01-01 00:00:00pages
73-80issue
1eissn
1040-452Xissn
1098-2795journal_volume
34pub_type
杂志文章abstract::In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199702)46:2<190::AID-MRD9>
更新日期:1997-02-01 00:00:00
abstract::An early step in mammalian fertilization is species-restricted binding of sperm to the egg's zona pellucida (ZP), a thick extracellular coat that surrounds eggs. Sperm bind to the ZP of unfertilized eggs, but not to the ZP of fertilized eggs. Shortly after binding to the unfertilized egg ZP, sperm undergo the acrosome...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21046
更新日期:2009-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<323::AID-MRD3>3.0.CO
更新日期:2000-12-01 00:00:00
abstract::Mitochondrial metabolic capacity and DNA replication have both been shown to affect oocyte quality, but it is unclear which one is more critical. In this study, immature oocytes were treated with FCCP or ddC to independently inhibit the respective mitochondrial metabolic capacity or DNA replication of oocytes during i...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22042
更新日期:2012-06-01 00:00:00
abstract::For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20705
更新日期:2007-10-01 00:00:00
abstract::The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20897
更新日期:2008-10-01 00:00:00
abstract::The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360313
更新日期:1993-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23344
更新日期:2020-05-01 00:00:00
abstract::The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080290303
更新日期:1991-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21092
更新日期:2009-12-01 00:00:00
abstract::Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330213
更新日期:1992-10-01 00:00:00
abstract::Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10065
更新日期:2002-05-01 00:00:00
abstract::In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20787
更新日期:2008-04-01 00:00:00
abstract::In vitro growth of preantral follicles has the potential to produce considerable numbers of competent oocytes for use in medicine, agriculture, and even wildlife conservation. The critical regulatory role of growth factors and hormones in the development of preantral follicles has been established. This study investig...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22131
更新日期:2013-01-01 00:00:00
abstract::The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199705)47:1<19::AID-MRD3>3
更新日期:1997-05-01 00:00:00
abstract::Several proteins with phosphorylated tyrosine residues have been shown to be closely involved in the control meiotic nuclear division. We identified a 42-kD protein in pig oocytes, using a polyclonal antibody to a synthetic phosphotyrosine construct that increases significantly in amount after 12 hr of maturation cult...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199607)44:3<408::AID-MRD14
更新日期:1996-07-01 00:00:00
abstract::Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20356
更新日期:2005-10-01 00:00:00
abstract::Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.23180
更新日期:2019-08-01 00:00:00
abstract::Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20809
更新日期:2008-05-01 00:00:00
abstract::Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199810)51:2<130::AID-MRD2>
更新日期:1998-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199608)44:4<476::AID-MRD7>
更新日期:1996-08-01 00:00:00
abstract::Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200004)55:4<422::AID-MRD10
更新日期:2000-04-01 00:00:00
abstract::Hexokinase is the first enzyme in the glycolytic pathway and utilizes ATP to convert glucose to glucose-6-phosphate (G6P). We previously identified three variant transcripts of Hk1 that are expressed specifically in spermatogenic cells, have different 5' untranslated regions, and encode a protein (HK1S, spermatogenic ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20791
更新日期:2008-04-01 00:00:00
abstract::The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Deltapsi) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21064
更新日期:2009-11-01 00:00:00
abstract::Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated t...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199910)54:2<112::AID-MRD2>
更新日期:1999-10-01 00:00:00
abstract::The aim of this study is to examine the effect of bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification. In Experiment 1, 4 groups of oocytes were used: (1) immature oo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1153
更新日期:2002-02-01 00:00:00
abstract::Sry expression is essential for initiating male sex differentiation, and the expression occurs only during a restricted period in the developing gonad. It is thought that Sry is part of a pathway of genes that regulate sex determination. Although the interactions of several genes with Sry expression have been suggeste...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10278
更新日期:2003-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22120
更新日期:2012-11-01 00:00:00
abstract::We have studied the presence of four sperm glycosidases, alpha-mannosidase, alpha-L- fucosidase and two beta-hexosaminidase isoforms, in 11 species of the genus Drosophila spanning approximately an evolutionary 60 MY period, and in Scaptodrosophila lebanonensis, belonging to the ancestor genus Scaptodrosophila. These ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20932
更新日期:2009-01-01 00:00:00
abstract::We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期:2000-12-01 00:00:00