Abstract：
:Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream applications. This study demonstrates that hESCs can be passaged as single cells using Accutase, a formulated mixture of digestive enzymes. In contrast to trypsin treatment, Accutase treatment does not significantly affect the viability and proliferation rate of hESC dissociation into single cells. Accutase-dissociated single cells can be separated by FACS and proliferate as fully pluripotent hESCs. An Oct4-eGFP reporter construct engineered into hESCs was used to monitor the pluripotency of hESCs passaged with Accutase. Compared to collagenase-passaged hESCs, Accutase-treated cultures contained a larger proportion of undifferentiated (Oct4-positive) cells. Additionally, Accutase-passaged undifferentiated hESCs could be grown as monolayers without the need for monitoring and/or selection for quality hESC colonies.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Bajpai R,Lesperance J,Kim M,Terskikh AVdoi
10.1002/mrd.20809subject
Has Abstractpub_date
2008-05-01 00:00:00pages
818-27issue
5eissn
1040-452Xissn
1098-2795journal_volume
75pub_type
杂志文章abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20674
更新日期：2007-09-01 00:00:00
abstract：:Ovorubin and PV2 are the major lipoglycocarotenoproteins present in the perivitellus of the freshwater snail eggs of Pomacea canaliculata, a rapidly expanding rice field pest. We have previously characterized these two particles regarding their lipid and protein compositions, their synthesis and tissular distribution,...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20078
更新日期：2004-07-01 00:00:00
abstract：:In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22187
更新日期：2013-07-01 00:00:00
abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21275
更新日期：2011-02-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199805)50:1<54::AID-MRD7>3
更新日期：1998-05-01 00:00:00
abstract：:In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22955
更新日期：2018-03-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：2011-09-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22120
更新日期：2012-11-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20314
更新日期：2005-08-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
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更新日期：2002-06-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080320313
更新日期：1992-07-01 00:00:00
abstract：:cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22329
更新日期：2014-07-01 00:00:00
abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330115
更新日期：1992-09-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22418
更新日期：2014-10-01 00:00:00
abstract：:So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20762
更新日期：2008-01-01 00:00:00
abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22307
更新日期：2014-05-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080280410
更新日期：1991-04-01 00:00:00
abstract：:Primordial germ cells (PGCs), the precursors of sperm or ova, could be used to generate transgenic animals and interspecies germ-line chimeras, which would facilitate the recovery of endangered species by making their access and manipulation in vitro easier. During early embryogenesis in avian species, PGCs are transp...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22802
更新日期：2017-06-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10234
更新日期：2003-04-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22516
更新日期：2015-10-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250306
更新日期：1990-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/mrd.20620
更新日期：2007-05-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199709)48:1<45::AID-MRD6>3
更新日期：1997-09-01 00:00:00
abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20207
更新日期：2005-03-01 00:00:00
abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22436
更新日期：2014-12-01 00:00:00
abstract：:In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199606)44:2<141::AID-MRD1>
更新日期：1996-06-01 00:00:00
abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22425
更新日期：2014-12-01 00:00:00
abstract：:Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific pho...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330308
更新日期：1992-11-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21092
更新日期：2009-12-01 00:00:00
abstract：:Hoechst 33342 (H342), in combination with ultraviolet (UV) irradiation, is frequently used to aid or confirm the enucleation of porcine oocytes in somatic cell nuclear transfer programs. The exposure of oocytes to H342 and UV irradiation has a deleterious effect on the development of in vitro-fertilized porcine oocyte...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22071
更新日期：2012-09-01 00:00:00
