Abstract:
:Sperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Microfilaments and the tubulin-like protein were associated with the sperm rays and spines, but were absent at the spike and at its base. Folded and unfolded spermatozoa display similar fluorescence patterns. SDS-PAGE of whole spermatozoa and electrotransfer to nitrocellulose confirmed the presence of actin and two proteins at 97 kd and 120 kd that bind to tubulin antibodies (tubulin-like proteins). These results demonstrate the presence of actin, but not tubulin, and localize microfilaments in these sperm. It is proposed that this cytoskeletal component is active in sperm during crustacean fertilization.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Perez C,Roco M,Castro A,Dupre E,Schatten G,Barros Cdoi
10.1002/mrd.1080280410subject
Has Abstractpub_date
1991-04-01 00:00:00pages
373-9issue
4eissn
1040-452Xissn
1098-2795journal_volume
28pub_type
杂志文章abstract::The aim of the present series of experiments was to investigate the effect of the size of follicle from which the oocytes originate on their subsequent in vitro developmental ability. Ovarian follicles were isolated and grouped according to size (2-6 mm, > 6 mm). Primary oocytes were carefully liberated and grouped ac...
journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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