Microtubule distribution during fertilization in the rabbit.

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...

journal_title：Molecular reproduction and development

authors： Keremu A,Yaoliwasi A,Tuerhong M,Kadeer N,Heyi,Yiming A,Yilike X

更新日期：2019-02-01 00:00:00

abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...

journal_title：Molecular reproduction and development

authors： Muscarella DE,Rachlinski MK,Bloom SE

更新日期：1998-10-01 00:00:00

abstract：:The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...

journal_title：Molecular reproduction and development

authors： Allan ER,Habibi HR

更新日期：2012-09-01 00:00:00

abstract：:Using alpha and beta spectrin mammalian antibodies on Western blotting, we demonstrated that lizard ovarian follicles contain two isoforms of alpha spectrin, Mr 94 and 134 kDa, and a 230 kDa beta spectrin, and that their pattern modifies in relation to pyriform cell differentiation. In fact, a positive immunoreaction ...

journal_title：Molecular reproduction and development

authors： Ricchiari L,Carmela VM,Marina P,Rosa C,Annamaria L,Piero A

更新日期：2004-01-01 00:00:00

abstract：:Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...

journal_title：Molecular reproduction and development

authors： Li BC,Tian ZQ,Sun M,Xu Q,Wang XY,Qin YR,Xu F,Gao B,Wang KH,Sun HC,Chen GH

更新日期：2010-09-01 00:00:00

abstract：:Ejaculated boar spermatozoa exhibit two types of hyperactivation: full and non-full. Full-type hyperactivation is characterized by the asymmetrical bending of the entire middle piece-principal piece and a twisting/figure-eight-like trajectory, and can be induced by simple incubation with CaCl2 after preincubation with...

journal_title：Molecular reproduction and development

authors： Otsuka N,Harayama H

更新日期：2017-11-01 00:00:00

abstract：:In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...

journal_title：Molecular reproduction and development

authors： Phillips KP,Baltz JM

更新日期：1996-09-01 00:00:00

abstract：:In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of...

journal_title：Molecular reproduction and development

authors： Ohashi A,Imai H,Minami N

更新日期：2003-12-01 00:00:00

abstract：:The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...

journal_title：Molecular reproduction and development

authors： Prather RS,Schatten G

更新日期：1992-07-01 00:00:00

abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...

journal_title：Molecular reproduction and development

authors： Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst J

更新日期：2006-08-01 00:00:00

abstract：:FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryogenesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-...

journal_title：Molecular reproduction and development

authors： Lamb KA,Johnson LR,Rizzino A

更新日期：1997-11-01 00:00:00

abstract：:Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnR...

journal_title：Molecular reproduction and development

authors： Kanka J,Fulka J Jr,Fulka J,Petr J

更新日期：1991-06-01 00:00:00

abstract：:The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...

journal_title：Molecular reproduction and development

authors： Ding NZ,Teng CB,Ma H,Ni H,Ma XH,Xu LB,Yang ZM

更新日期：2003-11-01 00:00:00

abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...

journal_title：Molecular reproduction and development

authors： Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FA

更新日期：2011-02-01 00:00:00

abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...

journal_title：Molecular reproduction and development

authors： Fraser LR,Hosseini R,Hanyalogou A,Talmor A,Dudley RK

更新日期：1997-11-01 00:00:00

abstract：:Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...

journal_title：Molecular reproduction and development

authors： Magnani L,Cabot RA

更新日期：2007-10-01 00:00:00

abstract：:Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...

journal_title：Molecular reproduction and development

authors： Shankar S,Mohapatra B,Verma S,Selvi R,Jagadish N,Suri A

更新日期：2004-10-01 00:00:00

abstract：:The first indication that the insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is developmentally regulated came from studies of the serum form of the receptor in the rat. By immunoblotting, the circulating form of the receptor, which was 10 kDa smaller than the tissue receptor, was high in 19 ...

journal_title：Molecular reproduction and development

authors： Nissley P,Kiess W,Sklar M

更新日期：1993-08-01 00:00:00

abstract：:Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...

journal_title：Molecular reproduction and development

authors： Zernicka-Goetz M

更新日期：1991-02-01 00:00:00

abstract：:Fragmentation occurs during early developmental stages of electrically activated oocytes and nuclear transfer (NT) embryos. It might contribute to the low developmental rate of porcine NT embryos. The present study was conducted to investigate whether the addition of sugars such as sorbitol or sucrose suppresses fragm...

journal_title：Molecular reproduction and development

authors： Im GS,Yang BS,Lai L,Liu Z,Hao Y,Prather RS

更新日期：2005-06-01 00:00:00

abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...

journal_title：Molecular reproduction and development

authors： Munsie M,Schlatt S,deKretser DM,Loveland KL

更新日期：1997-05-01 00:00:00

abstract：:In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...

journal_title：Molecular reproduction and development

authors： Wood TC,Montali RJ,Wildt DE

更新日期：1997-02-01 00:00:00

abstract：:Successful fertilization in mammals depends on the sperm's ability to initiate intracellular Ca2+ oscillations in the egg, a phenomenon that is elicited by Sperm-oocyte activating factors (SOAFs), whose quantitative and/or qualitative defect might result in fertilization failure. One such proposed factor is Post-acros...

journal_title：Molecular reproduction and development

authors： Freour T,Barragan M,Torra-Massana M,Ferrer-Vaquer A,Vassena R

更新日期：2018-02-01 00:00:00

abstract：:Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...

journal_title：Molecular reproduction and development

authors： Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

更新日期：1991-11-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:Abattoir-derived oocytes were exposed to a concentrated cryoprotectant solution (DAP213: 2 M DMSO, 1 M acetamide, 3 M propanediol, and 10% FCS in TCM199) for 1.5 or 5 min at the germinal vesicle (GV) stage or after maturation in vitro (IVM). Their viability was assessed by in vitro fertilization (IVF) and culture (IVC...

journal_title：Molecular reproduction and development

authors： Fuku EJ,Liu J,Downey BR

更新日期：1995-02-01 00:00:00

abstract：:Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex rev...

journal_title：Molecular reproduction and development

authors： Meyers-Wallen VN

更新日期：2003-08-01 00:00:00

abstract：:The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...

journal_title：Molecular reproduction and development

authors： Hyttel P,Vajta G,Callesen H

更新日期：2000-05-01 00:00:00

abstract：:Regulation of protein tyrosine phosphorylation is required for sperm capacitation and oocyte fertilization. The objective of the present work was to study the role of the calcium-sensing receptor (CaSR) on protein tyrosine phosphorylation in boar spermatozoa under capacitating conditions. To do this, boar spermatozoa ...

journal_title：Molecular reproduction and development

authors： Macías-García B,García-Marín LJ,Bragado MJ,González-Fernández L

更新日期：2019-07-01 00:00:00