Directional differentiation of chicken primordial germ cells into adipocytes, neuron-like cells, and osteoblasts.

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...

journal_title：Molecular reproduction and development

authors： Davis AF,Jeong-Yu S,Clayton DA

更新日期：1995-11-01 00:00:00

abstract：:Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...

journal_title：Molecular reproduction and development

authors： Cohen DJ,Rochwerger L,Ellerman DA,Morgenfeld MM,Busso D,Cuasnicú PS

更新日期：2000-06-01 00:00:00

abstract：:Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...

journal_title：Molecular reproduction and development

authors： Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

更新日期：1991-11-01 00:00:00

abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...

journal_title：Molecular reproduction and development

authors： Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst J

更新日期：2006-08-01 00:00:00

abstract：:In Ciona intestinalis, sperm penetration through the egg vitelline coat is an essential event of fertilization. We investigated whether trypsin- and chymotrypsin-like enzymes are involved in this event. Inhibitors and peptide substrates for chymotrypsin-like enzymes blocked the overall process of fertilization in a co...

journal_title：Molecular reproduction and development

authors： Pinto MR,Hoshi M,Marino R,Amoroso A,De Santis R

更新日期：1990-08-01 00:00:00

abstract：:Three sources of hamster periovulatory fluids (+/- heat inactivation at 56 degrees C), with bovine serum albumin (BSA) as control, were tested for effects on penetration of three classes of eggs by hamster sperm precapacitated in BSA. These fluids were a soluble extract of cumulus oophorus fluid (COF) from the ovulate...

journal_title：Molecular reproduction and development

authors： Boatman DE,Magnoni GE,Robbins RS

更新日期：1994-08-01 00:00:00

abstract：:Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...

journal_title：Molecular reproduction and development

authors： Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FA

更新日期：2011-02-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:Ovorubin and PV2 are the major lipoglycocarotenoproteins present in the perivitellus of the freshwater snail eggs of Pomacea canaliculata, a rapidly expanding rice field pest. We have previously characterized these two particles regarding their lipid and protein compositions, their synthesis and tissular distribution,...

journal_title：Molecular reproduction and development

authors： Dreon MS,Heras H,Pollero RJ

更新日期：2004-07-01 00:00:00

abstract：:Under the influence of gonadotropins or growth factors, a close cooperation develops between cumulus cells and the oocyte that is implicated in transmitting signals involved in maintaining or releasing the meiotic arrest in the oocyte. While cyclic adenosine 5'-monophosphate (cAMP) is a key molecule in maintaining the...

journal_title：Molecular reproduction and development

authors： Silvestre F,Boni R,Fissore RA,Tosti E

更新日期：2011-10-01 00:00:00

abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...

journal_title：Molecular reproduction and development

authors： Chen X,Yue Y,He Y,Zhu H,Hao H,Zhao X,Qin T,Wang D

更新日期：2014-10-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00

abstract：:The 26S proteasome is a self-compartmentalizing protease responsible for the degradation of intracellular proteins. This giant intracellular protease is formed by several subunits arranged into two 19S polar caps-where protein recognition and ATP-dependent unfolding occur-flanking a 20S central barrel-shaped structure...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2000-10-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...

journal_title：Molecular reproduction and development

authors： Murray MK,DeSouza MM

更新日期：1995-11-01 00:00:00

abstract：:Glutamine (GLN) is a metabolic precursor for hexosamine synthesis and its inclusion in culture medium has been reported to improve cumulus expansion. Glutamine and cysteine share the same transport system. Excess external GLN may act as a competitive inhibitor for the uptake of cysteine and stimulate loss of cellular ...

journal_title：Molecular reproduction and development

authors： Furnus CC,de Matos DG,Moses DF

更新日期：1998-09-01 00:00:00

abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...

journal_title：Molecular reproduction and development

authors： Boonkusol D,Gal AB,Bodo S,Gorhony B,Kitiyanant Y,Dinnyes A

更新日期：2006-06-01 00:00:00

abstract：:Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...

journal_title：Molecular reproduction and development

authors： Maiti A,Mishra KP,Majumder GC

更新日期：2008-07-01 00:00:00

abstract：:Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...

journal_title：Molecular reproduction and development

authors： Wang Y,Zhang JJ,Yang WR,Luo HY,Zhang JH,Wang XZ

更新日期：2015-11-01 00:00:00

abstract：:In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of...

journal_title：Molecular reproduction and development

authors： Ohashi A,Imai H,Minami N

更新日期：2003-12-01 00:00:00

abstract：:The aim of this study is to examine the effect of bovine oocyte maturation, fertilization or culture in vivo or in vitro on the proportion of oocytes reaching the blastocyst stage, and on blastocyst quality as measured by survival following vitrification. In Experiment 1, 4 groups of oocytes were used: (1) immature oo...

journal_title：Molecular reproduction and development

authors： Rizos D,Ward F,Duffy P,Boland MP,Lonergan P

更新日期：2002-02-01 00:00:00

abstract：:Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...

journal_title：Molecular reproduction and development

authors： Kim M,Geum D,Khang I,Park YM,Kang BM,Lee KA,Kim K

更新日期：2002-01-01 00:00:00

abstract：:In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...

journal_title：Molecular reproduction and development

authors： Kölle S,Dubois CS,Caillaud M,Lahuec C,Sinowatz F,Goudet G

更新日期：2007-07-01 00:00:00

abstract：:Mitochondrial metabolic capacity and DNA replication have both been shown to affect oocyte quality, but it is unclear which one is more critical. In this study, immature oocytes were treated with FCCP or ddC to independently inhibit the respective mitochondrial metabolic capacity or DNA replication of oocytes during i...

journal_title：Molecular reproduction and development

authors： Ge H,Tollner TL,Hu Z,Dai M,Li X,Guan H,Shan D,Zhang X,Lv J,Huang C,Dong Q

更新日期：2012-06-01 00:00:00

abstract：:The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...

journal_title：Molecular reproduction and development

authors： Ding NZ,Teng CB,Ma H,Ni H,Ma XH,Xu LB,Yang ZM

更新日期：2003-11-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growt...

journal_title：Molecular reproduction and development

authors： Scotti L,Parborell F,Irusta G,De Zuñiga I,Bisioli C,Pettorossi H,Tesone M,Abramovich D

更新日期：2014-08-01 00:00:00

abstract：:Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature ...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Zhang D,Pan Q,Ding M,Deng H

更新日期：2006-10-01 00:00:00

abstract：:We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...

journal_title：Molecular reproduction and development

authors： Tian XC,Lonergan P,Jeong BS,Evans AC,Yang X

更新日期：2002-05-01 00:00:00

abstract：:Sequential culture and coculture are two methods of improving the development of preimplantation embryos in vitro. Direct comparison of the efficiency of these methods is limited. Proliferation and apoptosis determine the total number of blastomere in preimplantation embryo, which is a sensitive parameter for evaluati...

journal_title：Molecular reproduction and development

authors： Xu JS,Chan ST,Lee WW,Lee KF,Yeung WS

更新日期：2004-05-01 00:00:00