Plac1 (placenta-specific 1) is essential for normal placental and embryonic development.

abstract：:A novel restriction fragment differential display (RFDD) RT-PCR has been used to compare patterns of mRNA expression in bovine oocytes matured in vitro in the presence (10%) or absence of fetal calf serum (FCS). Total RNA extracted from matured and denuded oocytes was processed using display Profile kit (Display Syste...

journal_title：Molecular reproduction and development

authors： Rzucidlo SJ,Gibbons J,Stice SL

更新日期：2001-05-01 00:00:00

abstract：:One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...

journal_title：Molecular reproduction and development

authors： Thaler CD,Thomas M,Ramalie JR

更新日期：2006-12-01 00:00:00

abstract：:Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...

journal_title：Molecular reproduction and development

authors： Brewis IA,Morton IE,Moore HD,England GC

更新日期：2001-12-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...

journal_title：Molecular reproduction and development

authors： Ochi H,Aoto S,Tachibana K,Hara M,Chiba K

更新日期：2016-01-01 00:00:00

abstract：:The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...

journal_title：Molecular reproduction and development

authors： Stuckas H,Messerschmidt K,Putzler S,Baumann O,Schenk JA,Tiedemann R,Micheel B

更新日期：2009-01-01 00:00:00

abstract：:PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...

journal_title：Molecular reproduction and development

authors： Massabbal E,Parveen S,Weisoly DL,Nelson DM,Smith SD,Fant M

更新日期：2005-07-01 00:00:00

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...

journal_title：Molecular reproduction and development

authors： Bianco B,Peluso C,Gava MM,Ghirelli-Filho M,Lipay MV,Lipay MA,Christofolini DM,Barbosa CP

更新日期：2011-09-01 00:00:00

abstract：:To improve in vitro maturation and to understand the mechanism for meiotic resumption of oocytes, meiotic progression, and its control by hypoxanthine (HX) were studied in goat oocytes. Ovaries were obtained from a local abattoir, and cumulus-oocyte complexes (COCs) and follicular fluid were collected from follicles o...

journal_title：Molecular reproduction and development

authors： Ma S,Lan G,Miao Y,Wang Z,Chang Z,Luo M,Tan J

更新日期：2003-11-01 00:00:00

abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...

journal_title：Molecular reproduction and development

authors： Roy A,Yan W,Burns KH,Matzuk MM

更新日期：2004-03-01 00:00:00

abstract：:Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI)...

journal_title：Molecular reproduction and development

authors： Shinmen A,Honda A,Ohkawa M,Hirose M,Ogonuki N,Yuzuriha M,Miki H,Mochida K,Inoue K,Abe K,Ito M,Ogura A

更新日期：2007-09-01 00:00:00

abstract：:Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...

journal_title：Molecular reproduction and development

authors： Lee JY,Lim JM,Kim DK,Zheng YH,Moon S,Han BK,Song KD,Kim H,Han JY

更新日期：2008-01-01 00:00:00

abstract：:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...

journal_title：Molecular reproduction and development

authors： Rambhatla L,Patel B,Dhanasekaran N,Latham KE

更新日期：1995-07-01 00:00:00

abstract：:Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...

journal_title：Molecular reproduction and development

authors： Berger T

更新日期：1990-11-01 00:00:00

abstract：:These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...

journal_title：Molecular reproduction and development

authors： Rickords LF,White KL,Wiltbank JN

更新日期：1990-10-01 00:00:00

abstract：:Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Lareyre JJ,Madigou T,Davail B,Jalabert B,Breton B

更新日期：2002-05-01 00:00:00

abstract：:While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...

journal_title：Molecular reproduction and development

authors： Lozano JC,Schatt P,Vergé V,Gobinet J,Villey V,Peaucellier G

更新日期：2010-05-01 00:00:00

abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...

journal_title：Molecular reproduction and development

authors： Benson S,Rawson R,Killian C,Wilt F

更新日期：1991-07-01 00:00:00

abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

journal_title：Molecular reproduction and development

authors： Xiong W,Wang Z,Shen C

更新日期：2019-08-01 00:00:00

abstract：:The uterus and the placenta synthesize insulin-like growth factors (IGFs) and insulin-like binding proteins (IGFBPs). These growth factors are implicated in processes of proliferation and differentiation that occur in the uterus. To determine the patterns of expression of IGFs during rat pregnancy we used in situ hybr...

journal_title：Molecular reproduction and development

authors： Correia-da-Silva G,Bell SC,Pringle JH,Teixeira N

更新日期：1999-07-01 00:00:00

abstract：:MAP kinase (MAPK) and its activator, MAP kinase kinase (MAPKK), are commonly activated by a variety of extracellular stimuli in mammalian cells and in the process of Xenopus oocyte maturation. In order to investigate the function of the MAPK cascade in oocyte maturation, we produced an anti-Xenopus MAPKK which specifi...

journal_title：Molecular reproduction and development

authors： Gotoh Y,Nishida E

更新日期：1995-12-01 00:00:00

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...

journal_title：Molecular reproduction and development

authors： Lee J,Park JI,Lee GS,Choi JH,Lee ST,Park CK,Kim DY,Hyun SH,Lee E

更新日期：2015-06-01 00:00:00

abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...

journal_title：Molecular reproduction and development

authors： Haendler B,Yamanouchi H,Lessey BA,Chwalisz K,Hess-Stumpp H

更新日期：2004-07-01 00:00:00

abstract：:The lack of a paternal genome in parthenogenetic embryos clearly limits their postimplantation development, but apparently not their preimplantation development, since morphologically normal blastocysts can be formed. The cleavage rate of these embryos during the preimplantation period gives a better indication of the...

journal_title：Molecular reproduction and development

authors： Henery CC,Kaufman MH

更新日期：1992-04-01 00:00:00

abstract：:Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...

journal_title：Molecular reproduction and development

authors： Murray MK,DeSouza MM

更新日期：1995-11-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...

journal_title：Molecular reproduction and development

authors： Phillips KP,Baltz JM

更新日期：1996-09-01 00:00:00

abstract：:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in ...

journal_title：Molecular reproduction and development

authors： Pawshe CH,Rao KB,Totey SM

更新日期：1998-03-01 00:00:00