Abstract:
:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produce chimeric honeybees by use of cell transplantation. Embryonic cells were transplanted between pre-gastrula stage embryos (32-34 hr after oviposition) and hatched larvae were reared in vitro for 4 days. Chimeric individuals were detected by use of microsatellite analysis and a conservative estimation approach. 4.8% of embryos, posteriorly injected with embryonic cells, developed into chimeric honeybee larvae. By injection of cells pre-stained with fluorescent cell tracer dye, we studied the integration of transplanted cells in the developing embryos. Number of injected cells varied from 0 to 50 and cells remained and multiplied mainly in the area of injection.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RHdoi
10.1002/mrd.20435keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
475-81issue
4eissn
1040-452Xissn
1098-2795journal_volume
73pub_type
杂志文章abstract::Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22020
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380409
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pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1080280410
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22066
更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280313
更新日期:1991-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199810)51:2<130::AID-MRD2>
更新日期:1998-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22771
更新日期:2017-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22802
更新日期:2017-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360411
更新日期:1993-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360109
更新日期:1993-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20612
更新日期:2007-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22516
更新日期:2015-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22686
更新日期:2016-09-01 00:00:00
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更新日期:2012-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400306
更新日期:1995-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1080420204
更新日期:1995-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.23123
更新日期:2019-10-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/(SICI)1098-2795(199605)44:1<36::AID-MRD4>3
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更新日期:1997-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20421
更新日期:2006-03-01 00:00:00
abstract::Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...
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pub_type: 杂志文章
doi:10.1002/mrd.1080270311
更新日期:1990-11-01 00:00:00