Abstract:
:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. Although progestogens sustained decidualization, endometrial breakdown was still observed in this model. We, therefore, hypothesized that endometrial breakdown might involve functional progesterone withdrawal. Using co-immunoprecipitation assays, we observed the constitutive activation of nuclear factor kappa-b (NF-κB) p65 and its interaction with the progesterone receptor (PGR); moreover, transcriptional activity of the PGR was also repressed by NF-κB activity in primary mouse and human decidual stromal cells that mimic progesterone maintenance. Yet the ratio of PGR-B to PGR-A was not increased in the mouse model. In vivo comparison of endometrial breakdown induced by progesterone withdrawal to that seen during sustained progesterone exposure, in the presence of NF-κB inhibitors, revealed that NF-κB-mediated functional progesterone withdrawal is involved in endometrial breakdown in this implant model. These data prompt further studies to determine the homology of this functional progesterone withdrawal mechanism in human endometrium. Mol. Reprod. Dev. 83: 780-791, 2016 © 2016 Wiley Periodicals, Inc.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YFdoi
10.1002/mrd.22686subject
Has Abstractpub_date
2016-09-01 00:00:00pages
780-791issue
9eissn
1040-452Xissn
1098-2795journal_volume
83pub_type
杂志文章abstract::Expression of ovine interferon-tau (oIFNtau), a factor essential for the process of maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast. However, the molecular mechanisms by which oIFNtau expression is restricted to the trophectoderm have not been fully elucidated. The objective o...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20457
更新日期:2006-05-01 00:00:00
abstract::Klinefelter syndrome (KS) is the most common genetic cause of human infertility, but the mechanism(s) responsible for its phenotype remain largely unknown. KS is associated with alterations in body composition and with a higher risk of developing metabolic diseases. We therefore hypothesized that KS men seeking fertil...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22608
更新日期:2016-03-01 00:00:00
abstract::Mouse oocyte development in vitro has been studied in the past several years, but no evidence showed that the fertilizable oocytes could be obtained from the fetal mouse germ cells before the formation of the primordial follicles. In this study, an efficient and simple method has been established to obtain the mature ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20535
更新日期:2006-10-01 00:00:00
abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00
abstract::We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199606)44:2<221::AID-MRD11
更新日期:1996-06-01 00:00:00
abstract::Oocyte maturation is accompanied by differentiation of surrounding cumulus cells. These cells produce hyaluronic acid (HA) and its storage in intercellular spaces results in expansion of the cells. The cumulus cells also accumulate cyclooxygenase-2 (cox-2) during maturation. Both expansion and cox-2 storage are regula...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10265
更新日期:2003-05-01 00:00:00
abstract::The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360313
更新日期:1993-11-01 00:00:00
abstract::The aims of this study were to investigate steady-state level of Kit Ligand (KL) mRNA and its effects on in vitro survival and growth of caprine preantral follicles. RT-PCR was used to analyze caprine steady-state level of KL mRNA in primordial, primary, and secondary follicles, and in small (1-3 mm) and large (3-6 mm...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21138
更新日期:2010-03-01 00:00:00
abstract::This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370304
更新日期:1994-03-01 00:00:00
abstract::Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420214
更新日期:1995-10-01 00:00:00
abstract::Glutamine (GLN) is a metabolic precursor for hexosamine synthesis and its inclusion in culture medium has been reported to improve cumulus expansion. Glutamine and cysteine share the same transport system. Excess external GLN may act as a competitive inhibitor for the uptake of cysteine and stimulate loss of cellular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199809)51:1<76::AID-MRD9>3
更新日期:1998-09-01 00:00:00
abstract::Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340404
更新日期:1993-04-01 00:00:00
abstract::In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22955
更新日期:2018-03-01 00:00:00
abstract::The distribution of microtubules was studied during fertilization of the rabbit oocyte by immunofluorescence microscopy after staining with an anti-alpha-tubulin antibody. In ovulated oocytes, microtubules were found exclusively in the meiotic spindle. At fertilization, the paternal centrosome generated sperm astral m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320313
更新日期:1992-07-01 00:00:00
abstract::Changes in the level of transcriptional activity in 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients were pulse labelled with 3H-uridine during cultivation in vitro, embryos were then fixed and processed for autoradiography and electron micros...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199602)43:2<135::AID-MRD1>
更新日期:1996-02-01 00:00:00
abstract::The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420407
更新日期:1995-12-01 00:00:00
abstract::Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22386
更新日期:2014-10-01 00:00:00
abstract::Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200009)57:1<88::AID-MRD12>3.0.CO
更新日期:2000-09-01 00:00:00
abstract::We have tested three parameters in sperm-mediated gene transfer assays with mice and pigs: (i) the epididymal versus ejaculated origin of sperm cells, (ii) the primary structure, and (iii) the amount of the challenging foreign DNA. We have found that the pVLCNhGH construct, of retrotransposon origin, causes a massive ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2+<301::AID-MRD2
更新日期:2000-06-01 00:00:00
abstract::The developmental competence of bovine follicular oocytes that had been meiotically arrested with the phosphokinase inhibitor 6-dimethylaminopurine (6-DMAP) was studied. After 24 h in vitro culture with 2 mM 6-DMAP, 85 +/- 12% of the oocytes were at the germinal vesicle stage compared to 97 +/- 3% at the start of cult...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199807)50:3<334::AID-MRD10
更新日期:1998-07-01 00:00:00
abstract::To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420107
更新日期:1995-09-01 00:00:00
abstract::In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200002)55:2<205::AID-MRD10
更新日期:2000-02-01 00:00:00
abstract::While most cyclin-dependent kinases (CDKs) are involved in cell cycle control, CDK5 is mostly known for crucial functions in neurogenesis. However, we cloned sea urchin CDK5 from a two-cell stage cDNA library and found that the protein is present in eggs and embryos, up to the pluteus stage, but without associated kin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21165
更新日期:2010-05-01 00:00:00
abstract::The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.1080320207
更新日期:1992-06-01 00:00:00
abstract::Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10128
更新日期:2002-09-01 00:00:00
abstract::PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20272
更新日期:2005-07-01 00:00:00
abstract::The lack of a paternal genome in parthenogenetic embryos clearly limits their postimplantation development, but apparently not their preimplantation development, since morphologically normal blastocysts can be formed. The cleavage rate of these embryos during the preimplantation period gives a better indication of the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080310406
更新日期:1992-04-01 00:00:00
abstract::Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1135
更新日期:2002-01-01 00:00:00
abstract::Fertile offspring from somatic cell nuclear transfer (SCNT) is the goal of most cloning laboratories. For this process to be successful, a number of events must occur correctly. First the donor nucleus must be in a state that is amenable to remodeling and subsequent genomic reprogramming. The nucleus must be introduce...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21242
更新日期:2010-12-01 00:00:00
abstract::The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization. Oocytes were aspirated from 2-5 mm follicles of ovaries collected at a local abattoir. The oocyte-cumulus complexes (OCCs) were cultur...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080310113
更新日期:1992-01-01 00:00:00