Effects of amino acids and alpha-amanitin on bovine embryo development in a simple protein-free medium.

abstract：:We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...

journal_title：Molecular reproduction and development

authors： Ojaghi M,Kastelic J,Thundathil J

更新日期：2017-05-01 00:00:00

abstract：:Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...

journal_title：Molecular reproduction and development

authors： Kaufman MH,Lee KK,Speirs S

更新日期：1990-04-01 00:00:00

abstract：:This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...

journal_title：Molecular reproduction and development

authors： Shalgi R,Magnus A,Jones R,Phillips DM

更新日期：1994-03-01 00:00:00

abstract：:FGF-4 appears to be the first fibroblast growth factor (FGF) expressed during embryogenesis, and its expression is critical for early mammalian development. FGF-4 is expressed in the embryonic cell lines, F9, D3, and NT2/D1; but its expression in these cells is repressed upon differentiation. Transcription of the FGF-...

journal_title：Molecular reproduction and development

authors： Lamb KA,Johnson LR,Rizzino A

更新日期：1997-11-01 00:00:00

abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

journal_title：Molecular reproduction and development

authors： Boni R

更新日期：2019-10-01 00:00:00

abstract：:Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...

journal_title：Molecular reproduction and development

authors： St Pierre J,Wright DJ,Rowe TC,Wright SJ

更新日期：2002-03-01 00:00:00

abstract：:Progesterone (P) has previously been shown to rapidly increase free intracellular calcium concentration ([Ca2+]i), and subsequently to initiate the acrosome reaction (AR) in capacitated human sperm. The present study used cytochemical analysis of the AR, and spectrofluorometric determination of sperm [Ca2+]i and intra...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1996-12-01 00:00:00

abstract：:We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...

journal_title：Molecular reproduction and development

authors： Agellon LB,Davies SL,Lin CM,Chen TT,Powers DA

更新日期：1988-01-01 00:00:00

abstract：:We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...

journal_title：Molecular reproduction and development

authors： Peippo J,Viitala S,Virta J,Räty M,Tammiranta N,Lamminen T,Aro J,Myllymäki H,Vilkki J

更新日期：2007-11-01 00:00:00

abstract：:Members of the Binder of SPerm (BSP) superfamily have been identified in both human and mouse epididymis. These proteins are known to bind sperm membrane and promote sperm capacitation. Studies suggest that BSPH2 might play a different role in sperm functions from its counterparts; however, the role of BSPH2 remains m...

journal_title：Molecular reproduction and development

authors： Eskandari-Shahraki M,Prud'homme B,Manjunath P

更新日期：2018-08-01 00:00:00

abstract：:The lack of a paternal genome in parthenogenetic embryos clearly limits their postimplantation development, but apparently not their preimplantation development, since morphologically normal blastocysts can be formed. The cleavage rate of these embryos during the preimplantation period gives a better indication of the...

journal_title：Molecular reproduction and development

authors： Henery CC,Kaufman MH

更新日期：1992-04-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...

journal_title：Molecular reproduction and development

authors： Cohen DJ,Rochwerger L,Ellerman DA,Morgenfeld MM,Busso D,Cuasnicú PS

更新日期：2000-06-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...

journal_title：Molecular reproduction and development

authors： Banliat C,Dubuisson F,Corbin E,Beurois J,Tomas D,Le Bourhis D,Salvetti P,Labas V,Mermillod P,Saint-Dizier M

更新日期：2019-06-01 00:00:00

abstract：:Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...

journal_title：Molecular reproduction and development

authors： Blomberg LA,Schreier LL,Talbot NC

更新日期：2008-03-01 00:00:00

abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...

journal_title：Molecular reproduction and development

authors： Celebi C,Auvray P,Benvegnu T,Plusquellec D,Jégou B,Guillaudeux T

更新日期：2002-08-01 00:00:00

abstract：:Cysteamine when added during in vitro maturation (IVM) or in vitro embryo culture (IVC) stimulates glutathione (GSH) synthesis and improves embryo developmental rates. This suggests that GSH synthesis is decreased in the in vitro produced embryo. The present study was carried out to evaluate if addition of cysteamine ...

journal_title：Molecular reproduction and development

authors： de Matos DG,Herrera C,Cortvrindt R,Smitz J,Van Soom A,Nogueira D,Pasqualini RS

更新日期：2002-06-01 00:00:00

abstract：:The highly conserved Pumilio protein plays crucial roles in fertility of many organisms acting as a repressor of translation, and causing infertility when mutated. Although one of two human Pumilio homologs, PUMILIO2 is expressed mainly in the germ line, its role in mammalian germ cell development has not been reporte...

journal_title：Molecular reproduction and development

authors： Kusz K,Ginter-Matuszewska B,Ziolkowska K,Spik A,Bierla J,Jedrzejczak P,Latos-Bielenska A,Pawelczyk L,Jaruzelska J

更新日期：2007-06-01 00:00:00

abstract：:In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the e...

journal_title：Molecular reproduction and development

authors： Yang BY,Greene M,Chen TT

更新日期：1999-06-01 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:Selection of transgenic embryos prior to embryo transfer is a means to increase the efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic expression of green fluorescent protein (GFP) has features that make it ideal for transgenic embryo selection. The primary objective of this study w...

journal_title：Molecular reproduction and development

authors： Chan AW,Kukolj G,Skalka AM,Bremel RD

更新日期：1999-04-01 00:00:00

abstract：:Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...

journal_title：Molecular reproduction and development

authors： Ralph JH,Telfer EE,Wilmut I

更新日期：1995-10-01 00:00:00

abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...

journal_title：Molecular reproduction and development

authors： Lane M,Bavister BD

更新日期：1999-12-01 00:00:00

abstract：:The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...

journal_title：Molecular reproduction and development

authors： Nishikimi H,Kansaku N,Saito N,Usami M,Ohno Y,Shimada K

更新日期：2000-01-01 00:00:00

abstract：:Oocyte maturation is accompanied by differentiation of surrounding cumulus cells. These cells produce hyaluronic acid (HA) and its storage in intercellular spaces results in expansion of the cells. The cumulus cells also accumulate cyclooxygenase-2 (cox-2) during maturation. Both expansion and cox-2 storage are regula...

journal_title：Molecular reproduction and development

authors： Vigneron C,Nuttinck F,Perreau C,Reinaud P,Charpigny G,Mermillod P

更新日期：2003-05-01 00:00:00

abstract：:This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time ...

journal_title：Molecular reproduction and development

authors： Choi JY,Kim CI,Park CK,Yang BK,Cheong HT

更新日期：2004-11-01 00:00:00

abstract：:We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...

journal_title：Molecular reproduction and development

authors： Tian XC,Lonergan P,Jeong BS,Evans AC,Yang X

更新日期：2002-05-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...

journal_title：Molecular reproduction and development

authors： Shinoda T,Miranda LA,Okuma K,Hattori RS,Fernandino JI,Yoshizaki G,Somoza GM,Strüssmann CA

更新日期：2010-06-01 00:00:00