Abstract:
:Selection of transgenic embryos prior to embryo transfer is a means to increase the efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic expression of green fluorescent protein (GFP) has features that make it ideal for transgenic embryo selection. The primary objective of this study was to assess cytoplasmic expression of a specially designed GFP reporter as a tool for transgenic bovine embryo selection. A second objective was to evaluate this reporter for studying transgenic mosaicism related to timing of integration of pronuclear microinjected DNA. Transgenic embryos produced by pronuclear injection showed a discrete pattern of GFP expression with clusters at 25, 50, and 100% of blastomeres expressing GFP. This pattern of mosaicism is interpreted to indicate that the integration of microinjected DNA occurred, not only at the pronuclear stage, but also in the subsequent cell divisions. Among the GFP-positive transgenic embryos, only in 21% did all the blastomeres show the green fluorescence. Using the fraction of positive blastomeres within an embryo, the timing of integration of microinjected DNA was estimated. The frequency of nonmosaic embryos expressing GFP is consistent with published germline transmission success rates of transgenic cattle derived from pronuclear microinjected embryos. These results indicate the possible application of GFP as a marker of transgenic embryos and graphically illustrate underlying complexities in DNA integration in embryos subjected to pronuclear microinjection.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Chan AW,Kukolj G,Skalka AM,Bremel RDdoi
10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
406-13issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>journal_volume
52pub_type
杂志文章abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.1080320304
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2010-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200101)58:1<45::AID-MRD7>3.0.CO;
更新日期:2001-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:1993-08-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380212
更新日期:1994-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10124
更新日期:2002-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22297
更新日期:2014-03-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.10293
更新日期:2003-05-01 00:00:00
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更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1143
更新日期:2002-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330115
更新日期:1992-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20272
更新日期:2005-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10278
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1998-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1063
更新日期:2001-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380203
更新日期:1994-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1991-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1994-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22052
更新日期:2012-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.23454
更新日期:2021-01-28 00:00:00