Abstract：
:This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time point after fusion without activation, or 3 or 7 hr after activation. Some nocodazole treated zygotes were fixed to analyze their chromosome constitutions. The proportion of eggs with a morphologically normal premature chromosome condensation (PCC) state increased 1-2 hr after fusion. Whereas eggs with elongated chromosome plate increased as activation time was prolonged to 3 hr, and 5 hr after fusion, 58.1% of eggs showed more than two scattered chromosome sets. The proportion of eggs with a single chromatin mass (40.6-56.7%) significantly increased when eggs were activated within 2.5 hr after fusion (P < 0.05). Only 23.3% of reconstituted embryos activated 5 hr after fusion formed one pronucleus-like structure (PN), whereas, 64.5-78.3% of embryos activated 1-2.5 hr after fusion formed one PN. The proportion of embryos with normal chromosome constitutions decreased as activation time was prolonged. Development rates to the blastocyst stage were higher in eggs activated within 2 hr after fusion (17.3-21.7%) compared to those of others (0-8.6%, P < 0.05). The result of the present study suggests that activation time can affect the chromatin structure and in vitro development of bovine nuclear transfer embryos.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Choi JY,Kim CI,Park CK,Yang BK,Cheong HTdoi
10.1002/mrd.20131keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
289-95issue
3eissn
1040-452Xissn
1098-2795journal_volume
69pub_type
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journal_title：Molecular reproduction and development
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pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1002/mrd.1125
更新日期：2002-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/mrd.20159
更新日期：2004-09-01 00:00:00
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pub_type： 杂志文章
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更新日期：2003-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/mrd.20844
更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/mrd.22498
更新日期：2015-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199805)50:1<54::AID-MRD7>3
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pub_type： 杂志文章,评审
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更新日期：2017-09-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1997-03-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2009-08-01 00:00:00
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更新日期：2000-12-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20712
更新日期：2008-01-01 00:00:00
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journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420214
更新日期：1995-10-01 00:00:00
