Testis-specific isoform of angiotensin-converting enzyme (tACE) is involved in the regulation of bovine sperm capacitation.

abstract：:In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...

journal_title：Molecular reproduction and development

authors： Elweza AE,Ezz MA,Acosta TJ,Talukder AK,Shimizu T,Hayakawa H,Shimada M,Imakawa K,Zaghloul AH,Miyamoto A

更新日期：2018-03-01 00:00:00

abstract：:Five experiments, utilizing 3741 embryos produced in vitro, were designed to test the effects of Eagle's nonessential amino acids, and combinations of Eagle's essential amino acids and the RNA polymerase inhibitor alpha-amanitin on the development of preimplantation bovine embryos in a modified protein-free KSOM mediu...

journal_title：Molecular reproduction and development

authors： Liu Z,Foote RH

更新日期：1997-03-01 00:00:00

abstract：:Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...

journal_title：Molecular reproduction and development

authors： Moses RM,Kline D

更新日期：1995-06-01 00:00:00

abstract：:To improve in vitro maturation and to understand the mechanism for meiotic resumption of oocytes, meiotic progression, and its control by hypoxanthine (HX) were studied in goat oocytes. Ovaries were obtained from a local abattoir, and cumulus-oocyte complexes (COCs) and follicular fluid were collected from follicles o...

journal_title：Molecular reproduction and development

authors： Ma S,Lan G,Miao Y,Wang Z,Chang Z,Luo M,Tan J

更新日期：2003-11-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...

journal_title：Molecular reproduction and development

authors： Stuckas H,Messerschmidt K,Putzler S,Baumann O,Schenk JA,Tiedemann R,Micheel B

更新日期：2009-01-01 00:00:00

abstract：:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...

journal_title：Molecular reproduction and development

authors： Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM

更新日期：1995-12-01 00:00:00

abstract：:The oviduct is a dynamic organ which modulates gamete physiology. Sperm-oviduct interaction provides the formation of a sperm storage reservoir and allows the selection of sperm with certain qualities in eutherian mammals. In sows, the oviductal sperm binding glycoprotein (SBG) has been proposed to be involved in sper...

journal_title：Molecular reproduction and development

authors： Teijeiro JM,Ignotz GG,Marini PE

更新日期：2009-04-01 00:00:00

abstract：:Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...

journal_title：Molecular reproduction and development

authors： Zhou J,Huang Y,Li L,Zhu L,Zhang D,Zhang S,Chen Y

更新日期：2013-11-01 00:00:00

abstract：:Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...

journal_title：Molecular reproduction and development

authors： Zernicka-Goetz M

更新日期：1991-02-01 00:00:00

abstract：:Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of M...

journal_title：Molecular reproduction and development

authors： Gu Y,He Y,Zhang X,Shi Y,Yang Q,Yu L,Sun Z,Zhang H,Wang J,Gao X,Wang J

更新日期：2015-06-01 00:00:00

abstract：:Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...

journal_title：Molecular reproduction and development

authors： Cohen DJ,Rochwerger L,Ellerman DA,Morgenfeld MM,Busso D,Cuasnicú PS

更新日期：2000-06-01 00:00:00

abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

journal_title：Molecular reproduction and development

authors： Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

更新日期：2007-09-01 00:00:00

abstract：:Global warming represents a major stressful environmental condition that compromises the reproductive efficiency of animals and humans via a rise of body temperature above its physiological homeothermic point (heat stress [HS]). The injuries caused by HS on reproductive function involves both male and female component...

journal_title：Molecular reproduction and development

authors： Boni R

更新日期：2019-10-01 00:00:00

abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...

journal_title：Molecular reproduction and development

authors： Sakakibara R,Sakai K,Sakurai Y,Kohnoura T,Ishiguro M

更新日期：1993-01-01 00:00:00

abstract：:Activins, the dimeric polypeptides of inhibin beta-subunits, exhibit paracrine effects on cell proliferation, differentiation, and various other cell functions. The complex biological response to activin appears to involve multiple receptors. In the present study, we examined the isoform mRNA expression of both activi...

journal_title：Molecular reproduction and development

authors： Wu TC,Jih MH,Wang L,Wan YJ

更新日期：1994-05-01 00:00:00

abstract：:Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...

journal_title：Molecular reproduction and development

authors： Alberio R,Kubelka M,Zakhartchenko V,Hajdúch M,Wolf E,Motlik J

更新日期：2000-04-01 00:00:00

abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...

journal_title：Molecular reproduction and development

authors： Acevedo N,Wang X,Dunn RL,Smith GD

更新日期：2007-02-01 00:00:00

abstract：:Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...

journal_title：Molecular reproduction and development

authors： Mújica A,Neri-Bazan L,Tash JS,Uribe S

更新日期：1994-07-01 00:00:00

abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

journal_title：Molecular reproduction and development

authors： Ahmad K,Naz RK

更新日期：1993-11-01 00:00:00

abstract：:A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Pan Q,Min L,Dong H,Deng J

更新日期：2006-05-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:The maturation of various aspects of sperm function have been demonstrated in monkey and human epididymal sperm, including the ability to undergo the acrosome reaction. The present study aimed to investigate the maturational changes in non-human primate sperm in the signal transduction mechanisms leading to the acroso...

journal_title：Molecular reproduction and development

authors： Yeung CH,Weinbauer GF,Cooper TG

更新日期：1999-10-01 00:00:00

abstract：:Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

journal_title：Molecular reproduction and development

authors： Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

更新日期：2006-09-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standard parthenogenetic agent. The exposure of ...

journal_title：Molecular reproduction and development

authors： Colonna R,Tatone C,Francione A,Rosati F,Callaini G,Corda D,Di Francesco L

更新日期：1997-10-01 00:00:00

abstract：:The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...

journal_title：Molecular reproduction and development

authors： Leighton PA,van de Lavoir MC,Diamond JH,Xia C,Etches RJ

更新日期：2008-07-01 00:00:00

abstract：:Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...

journal_title：Molecular reproduction and development

authors： Murray MK,DeSouza MM

更新日期：1995-11-01 00:00:00

abstract：:The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...

journal_title：Molecular reproduction and development

authors： Ding NZ,Teng CB,Ma H,Ni H,Ma XH,Xu LB,Yang ZM

更新日期：2003-11-01 00:00:00

abstract：:Spermiogenesis pursues three major objectives: (1) The safeguard of the male genome within the confines of a compact nucleus. (2) The accumulation of enzymes in the acrosome of be released at fertilization. (3) The development of a sperm propelling tail consisting of an axoneme surrounded by a scaffold of keratin-cont...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2001-08-01 00:00:00