Birth of correctly genotyped calves after multiplex marker detection from bovine embryo microblade biopsies.

abstract：:To study self-renewal, genetic modification, and differentiation of avian spermatogonial stem cells (SSCs), we isolated chicken SSCs from fetal testes on the 16th hatching day via enzyme digestion, and then cultured the SSCs over 2 months after purification in vitro. SSCs were identified by alkaline phosphatase staini...

journal_title：Molecular reproduction and development

authors： Yu F,Ding LJ,Sun GB,Sun PX,He XH,Ni LG,Li BC

更新日期：2010-04-01 00:00:00

abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...

journal_title：Molecular reproduction and development

authors： Liu J,Schoonjans L,Tielens S,Speleman F,Cornelissen M,De Sutter P,Dhont M,Van der Elst J

更新日期：2006-08-01 00:00:00

abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

journal_title：Molecular reproduction and development

authors： Valsangkar DS,Downs SM

更新日期：2015-09-01 00:00:00

abstract：:To study possible role and regulation of apoptosis occurred in primate endometrium, the expression of apoptosis-related molecules, Fas, FasL, B cell lymphoma/leukaemia-2 (Bcl-2), and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunoh...

journal_title：Molecular reproduction and development

authors： Wei P,Jin X,Tao SX,Han CS,Liu YX

更新日期：2005-04-01 00:00:00

abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...

journal_title：Molecular reproduction and development

authors： Sakakibara R,Sakai K,Sakurai Y,Kohnoura T,Ishiguro M

更新日期：1993-01-01 00:00:00

abstract：:Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...

journal_title：Molecular reproduction and development

authors： Chen X,Yue Y,He Y,Zhu H,Hao H,Zhao X,Qin T,Wang D

更新日期：2014-10-01 00:00:00

abstract：:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...

journal_title：Molecular reproduction and development

authors： Tourte M,Besse C,Mounolou JC

更新日期：1991-12-01 00:00:00

abstract：:Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...

journal_title：Molecular reproduction and development

authors： Kim M,Geum D,Khang I,Park YM,Kang BM,Lee KA,Kim K

更新日期：2002-01-01 00:00:00

abstract：:Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...

journal_title：Molecular reproduction and development

authors： Li BC,Tian ZQ,Sun M,Xu Q,Wang XY,Qin YR,Xu F,Gao B,Wang KH,Sun HC,Chen GH

更新日期：2010-09-01 00:00:00

abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...

journal_title：Molecular reproduction and development

authors： Van Stekelenburg-Hamers AE,Van Achterberg TA,Rebel HG,Fléchon JE,Campbell KH,Weima SM,Mummery CL

更新日期：1995-04-01 00:00:00

abstract：:Progesterone (P) has previously been shown to rapidly increase free intracellular calcium concentration ([Ca2+]i), and subsequently to initiate the acrosome reaction (AR) in capacitated human sperm. The present study used cytochemical analysis of the AR, and spectrofluorometric determination of sperm [Ca2+]i and intra...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1996-12-01 00:00:00

abstract：:Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 pro...

journal_title：Molecular reproduction and development

authors： Lee JY,Lim JM,Kim DK,Zheng YH,Moon S,Han BK,Song KD,Kim H,Han JY

更新日期：2008-01-01 00:00:00

abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...

journal_title：Molecular reproduction and development

authors： Lee J,Park JI,Lee GS,Choi JH,Lee ST,Park CK,Kim DY,Hyun SH,Lee E

更新日期：2015-06-01 00:00:00

abstract：:The objective of the present study was to determine whether Fyn kinase participated in signaling events during sperm-egg interactions, sperm incorporation, and meiosis II. The functional requirement of Fyn kinase activity in these events was tested through the use of the protein kinase inhibitor SKI-606 (Bosutinib) an...

journal_title：Molecular reproduction and development

authors： Luo J,McGinnis LK,Kinsey WH

更新日期：2009-09-01 00:00:00

abstract：:We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT ge...

journal_title：Molecular reproduction and development

authors： Matsumoto K,Kakidani H,Takahashi A,Nakagata N,Anzai M,Matsuzaki Y,Takahashi Y,Miyata K,Utsumi K,Iritani A

更新日期：1993-09-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...

journal_title：Molecular reproduction and development

authors： Lequarré AS,Feugang JM,Malhomme O,Donnay I,Massip A,Dessy F,Van Langendonckt A

更新日期：2001-01-01 00:00:00

abstract：:Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...

journal_title：Molecular reproduction and development

authors： Moses RM,Kline D

更新日期：1995-06-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

journal_title：Molecular reproduction and development

authors： Tian C,Liu J

更新日期：2013-09-01 00:00:00

abstract：:The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal ...

journal_title：Molecular reproduction and development

authors： Flytzanis CN,Bogosian EA,Niemeyer CC

更新日期：1989-01-01 00:00:00

abstract：:Integrins are a family of cell adhesion receptors involved in many cell-cell and cell-matrix interactions. Some of these heterodimeric receptors, such as alpha 5 beta 1 and alpha v beta 1, specifically recognize the amino acid sequence Arg-Gly-Asp (RGD) within their ligands. The RGD sequence is found in fibronectin, v...

journal_title：Molecular reproduction and development

authors： Fusi FM,Vignali M,Gailit J,Bronson RA

更新日期：1993-10-01 00:00:00

abstract：:The first indication that the insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is developmentally regulated came from studies of the serum form of the receptor in the rat. By immunoblotting, the circulating form of the receptor, which was 10 kDa smaller than the tissue receptor, was high in 19 ...

journal_title：Molecular reproduction and development

authors： Nissley P,Kiess W,Sklar M

更新日期：1993-08-01 00:00:00

abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...

journal_title：Molecular reproduction and development

authors： Kennedy KL,Floyd AA,Clarkson AM,Lee VH

更新日期：2003-01-01 00:00:00

abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...

journal_title：Molecular reproduction and development

authors： Boonkusol D,Gal AB,Bodo S,Gorhony B,Kitiyanant Y,Dinnyes A

更新日期：2006-06-01 00:00:00

abstract：:Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...

journal_title：Molecular reproduction and development

authors： Fraser LR,Hosseini R,Hanyalogou A,Talmor A,Dudley RK

更新日期：1997-11-01 00:00:00

abstract：:The objective of the present study was to determine whether glucocorticoid (GC) and its receptor (GC-R) are expressed in the porcine corpus luteum (CL), and whether GC influences porcine luteal hormone production. The gene expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11-HSD1), type 2 (11-HSD2), GC-R, and...

journal_title：Molecular reproduction and development

authors： Sakumoto R,Ito S,Okuda K

更新日期：2008-05-01 00:00:00

abstract：:The sequence of ionic changes involved in initiation of acrosomal exocytosis in capacitated mouse spermatozoa was investigated. Earlier studies demonstrated that a large influx of Na+ is required for exocytosis, this Na+ apparently being associated with an increase in intracellular pH (pHi) via an Na(+)-H+ exchanger. ...

journal_title：Molecular reproduction and development

authors： Fraser LR

更新日期：1993-11-01 00:00:00

abstract：:Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...

journal_title：Molecular reproduction and development

authors： Yoshida N,Mita K,Yamashita M

更新日期：2000-09-01 00:00:00

abstract：:Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

journal_title：Molecular reproduction and development

authors： Manandhar G,Schatten G

更新日期：2000-08-01 00:00:00

abstract：:DNA methylation/demethylation of donor genomes in recipient ooplasm after nuclear transfer occurs in a species-specific way. In cloned rabbit and bovine embryos, repetitive sequences maintain the donor-type methylation status, but typical demethylation of repetitive sequences takes place in cloned porcine embryos. To ...

journal_title：Molecular reproduction and development

authors： Chen T,Zhang YL,Jiang Y,Liu JH,Schatten H,Chen DY,Sun QY

更新日期：2006-03-01 00:00:00