Abstract:
:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penetration of the antibody into the cells. In the cytoplasm of all previtellogenic oocytes, a dense network of criss-crossed long microtubules was associated in a basket-like structure surrounding the mitochondrial mass. At the beginning of vitellogenesis, the network meshes enlarged, while clusters of mitochondria migrated, in close association with microtubule bundles. At the beginning of vitellogenesis, the reorganization of the microtubular network, mostly in the vegetal hemisphere, occurred during the segregation of the mitochondrial populations. Reorganization is characterized by (1) a temporary enlargement of the network and close association of mitochondrial clusters with microtubular bundles, and (2) a progressive organization of a ring-shaped microtubular structure in the crown elaboration area. It is hypothesized that these modifications of the microtubular cytoskeleton contribute to the maintenance of cell shape and the polarized organization of the cell.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Tourte M,Besse C,Mounolou JCdoi
10.1002/mrd.1080300410subject
Has Abstractpub_date
1991-12-01 00:00:00pages
353-9issue
4eissn
1040-452Xissn
1098-2795journal_volume
30pub_type
杂志文章abstract::An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350307
更新日期:1993-07-01 00:00:00
abstract::We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22790
更新日期:2017-05-01 00:00:00
abstract::An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340308
更新日期:1993-03-01 00:00:00
abstract::Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10143
更新日期:2002-08-01 00:00:00
abstract::Sex-related growth rate differences in preimplantation mouse embryos were investigated. In experiment I, Day 3 embryos were recovered from reproductive tracts, classified according to developmental stage, and cultured for 24 hr in CZB medium containing glucose. Each embryo was then reclassified and stained for measure...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400108
更新日期:1995-01-01 00:00:00
abstract::There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20712
更新日期:2008-01-01 00:00:00
abstract::Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22771
更新日期:2017-03-01 00:00:00
abstract::Hyperactivated motility was studied in guinea pig spermatozoa. In the presence of the local anesthetic procaine, a high number of sperm cells (64%) showed hyperactivation when incubated in minimal culture medium with pyruvate, lactate, and glucose. Hyperactivated motility was dependent on glucose in the medium. Sperm ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380309
更新日期:1994-07-01 00:00:00
abstract::Gamete membrane interaction is critical to initiate the development of a new organism. The signaling pathways governing this event, however, are poorly understood. In this report, we provide the first evidence that protein kinase C activity in mouse eggs plays a crucial role in the regulation of this process. Stimulat...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20718
更新日期:2007-11-01 00:00:00
abstract::In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199906)53:2<127::AID-MRD1>
更新日期:1999-06-01 00:00:00
abstract::In newt testis, spermatocytes never appear during winter, because secondary spermatogonia die by apoptosis just before meiosis. In the current study, we examined the effect of low temperatures on spermatogenesis. Incubation of newts at low temperatures (8, 12, 15 degrees C) induced defects in spermatogenesis in a temp...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10328
更新日期:2003-09-01 00:00:00
abstract::Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420303
更新日期:1995-11-01 00:00:00
abstract::We compared the results of intracytoplasmic sperm injection (ICSI) that leads to full term development of hybrid (B6C3F1 and B6D2F1) and inbred (C57BL/6) mouse embryos. Although fertilization and pre-implantation development of C57BL/6 eggs were similar to those of F1 hybrid eggs, post-implantation development of the ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1063
更新日期:2001-09-01 00:00:00
abstract::Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199706)47:2<181::AID-MRD9>
更新日期:1997-06-01 00:00:00
abstract::A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330115
更新日期:1992-09-01 00:00:00
abstract::We have studied the presence of four sperm glycosidases, alpha-mannosidase, alpha-L- fucosidase and two beta-hexosaminidase isoforms, in 11 species of the genus Drosophila spanning approximately an evolutionary 60 MY period, and in Scaptodrosophila lebanonensis, belonging to the ancestor genus Scaptodrosophila. These ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20932
更新日期:2009-01-01 00:00:00
abstract::Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22227
更新日期:2013-11-01 00:00:00
abstract::Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080270311
更新日期:1990-11-01 00:00:00
abstract::In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20678
更新日期:2007-08-01 00:00:00
abstract::Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330315
更新日期:1992-11-01 00:00:00
abstract::Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22649
更新日期:2016-06-01 00:00:00
abstract::We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280405
更新日期:1991-04-01 00:00:00
abstract::Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1029
更新日期:2001-07-01 00:00:00
abstract::The uterus and the placenta synthesize insulin-like growth factors (IGFs) and insulin-like binding proteins (IGFBPs). These growth factors are implicated in processes of proliferation and differentiation that occur in the uterus. To determine the patterns of expression of IGFs during rat pregnancy we used in situ hybr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199907)53:3<294::AID-MRD5>
更新日期:1999-07-01 00:00:00
abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20762
更新日期:2008-01-01 00:00:00
abstract::The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23454
更新日期:2021-01-28 00:00:00
abstract::A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320404
更新日期:1992-08-01 00:00:00
abstract::The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<323::AID-MRD3>3.0.CO
更新日期:2000-12-01 00:00:00
abstract::In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP-dependent protein kinase in sperm motion. In the flagellum, a human-specific isoform of the RII alpha subunit was located on the a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080390410
更新日期:1994-12-01 00:00:00
abstract::RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420313
更新日期:1995-11-01 00:00:00