Abstract:
:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follicles, but was undetectable in primordial follicles. Abundant expression of Cx43 mRNA was maintained in the granulosa cells of growing follicles through maturity. Granulosa cells were isolated from early preantral follicles and maintained in monolayer cultures for 72 hr. After the first 24 hr of culture, they were maintained for 48 hr in serum-free medium supplemented with 0, 1, 5, or 10 ng/ml of mouse EGF. Granulosa cell proteins were isolated, solubilized, and evaluated for Cx43 by Western blot analysis using antibodies to rat Cx43. Relative amounts of Cx43 protein (both phosphorylated and nonphosphorylated) were increased (P < 0.05) by EGF in a dose-dependent manner. Northern blot analysis of RNA from cultured granulosa cells demonstrated increased amounts of Cx43 mRNA in the EGF treated cultures (10 ng EGF/ml) relative to controls (P < 0.03). In summary, Cx43 gap junctions are synthesized in granulosa cells following the onset of folliculogenesis in vivo and their expression is enhanced by EGF in vitro.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Kennedy KL,Floyd AA,Clarkson AM,Lee VHdoi
10.1002/mrd.10219keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
61-9issue
1eissn
1040-452Xissn
1098-2795journal_volume
64pub_type
杂志文章abstract::The IGFs (IGF-I and IGF-II) are essential for normal mammalian growth and development. Their actions are mediated primarily by their interactions with the type I IGF receptor (IGF-I receptor), a transmembrane tyrosine kinase. The ligands and the IGF-I receptor are structurally related to insulin and to the insulin rec...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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journal_title:Molecular reproduction and development
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更新日期:1996-12-01 00:00:00
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
doi:10.1002/mrd.20512
更新日期:2006-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1000
更新日期:2001-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/1098-2795(200007)56:3<424::AID-MRD13>3.0.C
更新日期:2000-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22425
更新日期:2014-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1080370208
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pub_type: 杂志文章
doi:10.1002/mrd.20079
更新日期:2004-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420107
更新日期:1995-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199609)45:1<52::AID-MRD8>3
更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2001-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Molecular reproduction and development
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更新日期:2006-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.20697
更新日期:2007-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1990-02-01 00:00:00