Phylogeny of the insulin-like growth factors (IGFs) and receptors: a molecular approach.

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Derivation and stable maintenance of porcine induced pluripotent stem cells (piPSCs) is challenging. We herein systematically analyzed two piPSC lines, derived by lentiviral transduction and cultured under either leukemia inhibitory factor (LIF) or fibroblast growth factor (FGF) conditions, to shed more light on the u...

journal_title：Molecular reproduction and development

authors： Secher JO,Ceylan A,Mazzoni G,Mashayekhi K,Li T,Muenthaisong S,Nielsen TT,Li D,Li S,Petkov S,Cirera S,Luo Y,Thombs L,Kadarmideen HN,Dinnyes A,Bolund L,Roelen BA,Schmidt M,Callesen H,Hyttel P,Freude KK

更新日期：2017-03-01 00:00:00

abstract：:Hoechst 33342 (H342), in combination with ultraviolet (UV) irradiation, is frequently used to aid or confirm the enucleation of porcine oocytes in somatic cell nuclear transfer programs. The exposure of oocytes to H342 and UV irradiation has a deleterious effect on the development of in vitro-fertilized porcine oocyte...

journal_title：Molecular reproduction and development

authors： Gil MA,Maside C,Cuello C,Parrilla I,Vazquez JM,Roca J,Martinez EA

更新日期：2012-09-01 00:00:00

abstract：:Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...

journal_title：Molecular reproduction and development

authors： Haskell RE,Bowen RA

更新日期：1995-03-01 00:00:00

abstract：:Glutamine (GLN) is a metabolic precursor for hexosamine synthesis and its inclusion in culture medium has been reported to improve cumulus expansion. Glutamine and cysteine share the same transport system. Excess external GLN may act as a competitive inhibitor for the uptake of cysteine and stimulate loss of cellular ...

journal_title：Molecular reproduction and development

authors： Furnus CC,de Matos DG,Moses DF

更新日期：1998-09-01 00:00:00

abstract：:The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...

journal_title：Molecular reproduction and development

authors： Prather RS,Schatten G

更新日期：1992-07-01 00:00:00

abstract：:The presence of retinoic acid (RA) during in vitro maturation (IVM) improves bovine oocyte quality and developmental potential. In this work, we investigated the underlying molecular mechanisms. Cumulus-oocyte complexes were meiotically arrested by roscovitine and/or matured in defined medium containing RA, 1% ethanol...

journal_title：Molecular reproduction and development

authors： Gomez E,Rodríguez A,Goyache F,Díez C,José Royo L,Moreira PN,Néstor Caamaño J,Morán E,Gutiérrez-Adán A

更新日期：2004-09-01 00:00:00

abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...

journal_title：Molecular reproduction and development

authors： Boonkusol D,Gal AB,Bodo S,Gorhony B,Kitiyanant Y,Dinnyes A

更新日期：2006-06-01 00:00:00

abstract：:Mouse zygotes or halves of zygotes, containing either a female or a male pronucleus, were fused with ovulated metaphase II oocytes. In 59.7% of the resulting hybrid cells, the pronuclei underwent premature chromosome condensation (PCC). In some of these heterokaryons the 2 pronuclei differed in the dynamics of condens...

journal_title：Molecular reproduction and development

authors： Ciemerych MA,Czołowska R

更新日期：1993-01-01 00:00:00

abstract：:Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...

journal_title：Molecular reproduction and development

authors： Zhou J,Huang Y,Li L,Zhu L,Zhang D,Zhang S,Chen Y

更新日期：2013-11-01 00:00:00

abstract：:Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at ...

journal_title：Molecular reproduction and development

authors： Rossi P,Dolci S,Albanesi C,Grimaldi P,Geremia R

更新日期：1993-04-01 00:00:00

abstract：:Following Northern analysis, GGT mRNA was found predominantly within the caput epididymides and kidney. The size of mRNAs for kidney, caput, corpus, and ductus deferens were 2.2, 2.3, 2.2, and 2.3 kb, respectively, whereas cauda showed a doublet of 2.2 and 2.3 kb. GGT transpeptidation and hydrolytic activity within ep...

journal_title：Molecular reproduction and development

authors： Hinton BT,Palladino MA,Mattmueller DR,Bard D,Good K

更新日期：1991-01-01 00:00:00

abstract：:Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...

journal_title：Molecular reproduction and development

authors： Martínez ML,Martelotto L,Cabada MO

更新日期：2000-10-01 00:00:00

abstract：:We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT ge...

journal_title：Molecular reproduction and development

authors： Matsumoto K,Kakidani H,Takahashi A,Nakagata N,Anzai M,Matsuzaki Y,Takahashi Y,Miyata K,Utsumi K,Iritani A

更新日期：1993-09-01 00:00:00

abstract：:There are always more evidences indicating that 17beta-estradiol (E2) is to be necessary for normal male fertility. Here we report the expression of the most ubiquitously expressed member of the akt family of genes, akt1, in the lizard (Podarcis s. sicula) testis. We have used a nonmammalian vertebrate model (the liza...

journal_title：Molecular reproduction and development

authors： Russo M,Troncone G,Guarino FM,Angelini F,Chieffi P

更新日期：2005-05-01 00:00:00

abstract：:In the dasyurid marsupial, Sminthopsis crassicaudata, the mature spermatozoon has an inner homogeneous (C1) and a peripheral indented (C2) region. Using DNase-gold conjugates, and biotinylated genomic DNA probes, DNA was found to occur in both C1 and C2 regions. The morphogenesis of the spermatozoon nucleus was invest...

journal_title：Molecular reproduction and development

authors： Soon LL,Breed WG

更新日期：1996-02-01 00:00:00

abstract：:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast...

journal_title：Molecular reproduction and development

authors： Li GP,Chen DY,Lian L,Sun QY,Wang MK,Liu JL,Li JS,Han ZM

更新日期：2001-02-01 00:00:00

abstract：:In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of...

journal_title：Molecular reproduction and development

authors： Ohashi A,Imai H,Minami N

更新日期：2003-12-01 00:00:00

abstract：:To study self-renewal, genetic modification, and differentiation of avian spermatogonial stem cells (SSCs), we isolated chicken SSCs from fetal testes on the 16th hatching day via enzyme digestion, and then cultured the SSCs over 2 months after purification in vitro. SSCs were identified by alkaline phosphatase staini...

journal_title：Molecular reproduction and development

authors： Yu F,Ding LJ,Sun GB,Sun PX,He XH,Ni LG,Li BC

更新日期：2010-04-01 00:00:00

abstract：:Using alpha and beta spectrin mammalian antibodies on Western blotting, we demonstrated that lizard ovarian follicles contain two isoforms of alpha spectrin, Mr 94 and 134 kDa, and a 230 kDa beta spectrin, and that their pattern modifies in relation to pyriform cell differentiation. In fact, a positive immunoreaction ...

journal_title：Molecular reproduction and development

authors： Ricchiari L,Carmela VM,Marina P,Rosa C,Annamaria L,Piero A

更新日期：2004-01-01 00:00:00

abstract：:An exogenous supply of hematopoietic cytokines is essential for maintaining murine embryonic stem (ES) cells in a proliferative yet undifferentiated state. Recently, it was demonstrated that hematopoietic cytokines utilize the gp130 signal transduction pathway to maintain this phenotype, although their involvement tow...

journal_title：Molecular reproduction and development

authors： Moore K,Piedrahita JA

更新日期：1996-10-01 00:00:00

abstract：:Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...

journal_title：Molecular reproduction and development

authors： Russell DF,Ibáñez E,Albertini DF,Overström EW

更新日期：2005-10-01 00:00:00

abstract：:Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...

journal_title：Molecular reproduction and development

authors： Gimenez-Bonafé P,Ribes E,Zamora MJ,Kasinsky HE,Chiva M

更新日期：2002-07-01 00:00:00

abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

journal_title：Molecular reproduction and development

authors： Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

更新日期：2007-09-01 00:00:00

abstract：:In the lizard Podarcis sicula, the major vitellogenin (VTG)-derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were i...

journal_title：Molecular reproduction and development

authors： Romano M,Rosanova P,Anteo C,Limatola E

更新日期：2002-11-01 00:00:00

abstract：:The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...

journal_title：Molecular reproduction and development

authors： Stuckas H,Messerschmidt K,Putzler S,Baumann O,Schenk JA,Tiedemann R,Micheel B

更新日期：2009-01-01 00:00:00

abstract：:We previously showed that, during mouse oocyte maturation, specific maternal mRNAs (actins) are deadenylated, while others (hypoxanthine phosphoribosyltransferase:HPRT) are adenylated. As in other systems, these changes can be correlated with changes in translational activities. Maturation-specific polyadenylation in ...

journal_title：Molecular reproduction and development

authors： Paynton BV,Bachvarova R

更新日期：1994-02-01 00:00:00

abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

journal_title：Molecular reproduction and development

authors： Soto P,Smith LC

更新日期：2009-07-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...

journal_title：Molecular reproduction and development

authors： Rambhatla L,Patel B,Dhanasekaran N,Latham KE

更新日期：1995-07-01 00:00:00