Efficient production of transgenic cattle by retroviral infection of early embryos.

abstract：:The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Deltapsi) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial...

journal_title：Molecular reproduction and development

authors： Zhao XM,Fu XW,Hou YP,Yan CL,Suo L,Wang YP,Zhu HB,Dinnyés A,Zhu SE

更新日期：2009-11-01 00:00:00

abstract：:The nuclear matrix is thought to be responsible for DNA organization, DNA replication, RNA synthesis, and RNA processing. We have looked for the presence of nuclear matrix antigens during early mouse embryogenesis. Antibodies to peripheral and interior antigens (P1, Pl1, Pl2, and lamin B) were used to immunolocalize n...

journal_title：Molecular reproduction and development

authors： Prather RS,Schatten G

更新日期：1992-07-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:Teleost fish exhibit remarkably diverse and plastic patterns of sexual development. One of the most fascinating modes of plasticity is functional sex change, which is widespread in marine fish including species of commercial importance; however, the regulatory mechanisms remain elusive. In this review, we explore such...

journal_title：Molecular reproduction and development

authors： Liu H,Todd EV,Lokman PM,Lamm MS,Godwin JR,Gemmell NJ

更新日期：2017-02-01 00:00:00

abstract：:Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...

journal_title：Molecular reproduction and development

authors： Martínez ML,Martelotto L,Cabada MO

更新日期：2000-10-01 00:00:00

abstract：:Communication between the oocyte and its somatic cells has been shown to be important in oocyte development. Here we examined how the oocyte may be involved in bovine cumulus cell expansion. Intact bovine cumulus oocyte complexes (COC) were obtained by puncturing antral follicles. From the intact COC, oocytectomised c...

journal_title：Molecular reproduction and development

authors： Ralph JH,Telfer EE,Wilmut I

更新日期：1995-10-01 00:00:00

abstract：:The aim of this study was to determine the influence of hamster oviductal glycoprotein (oviductin) on in vitro gamete interaction. Oviductin was purified from the oviducts using lithium 3,5-diiodosalicylate, followed by phenol extraction. Immunocytochemistry using indirect fluorescence staining revealed that oviductin...

journal_title：Molecular reproduction and development

authors： Kimura H,Matsuda J,Ogura A,Asano T,Naiki M

更新日期：1994-11-01 00:00:00

abstract：:Protectin (CD59) is a complement regulatory protein which blocks the membrane attack complex during complement activation. CD59 was identified on the human sperm surface by means of H19, an IgG1 anti-protectin mouse monoclonal antibody. Using indirect immunofluorescence, flow cytometry and immunoperoxidase, CD59 was f...

journal_title：Molecular reproduction and development

authors： Fenichel P,Cervoni F,Hofmann P,Deckert M,Emiliozzi C,Hsi BL,Rossi B

更新日期：1994-07-01 00:00:00

abstract：:We have examined the developmental pattern of prolactin receptor expression in the mouse by reverse transcription-polymerase chain reaction, in situ hybridization, and radioligand binding and have found two unexpected aspects of temporal regulation. First, high levels of prolactin receptor mRNA were detected in mouse ...

journal_title：Molecular reproduction and development

authors： Tzeng SJ,Linzer DI

更新日期：1997-09-01 00:00:00

abstract：:Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...

journal_title：Molecular reproduction and development

authors： Kennedy KL,Floyd AA,Clarkson AM,Lee VH

更新日期：2003-01-01 00:00:00

abstract：:In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...

journal_title：Molecular reproduction and development

authors： Cabot B,Tseng YC,Crodian JS,Cabot R

更新日期：2017-12-01 00:00:00

abstract：:The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium-sized follicles (3-8 mm in diameter) were untreated (MCO) or treated with ...

journal_title：Molecular reproduction and development

authors： You J,Kim J,Lee H,Hyun SH,Hansen PJ,Lee E

更新日期：2012-01-01 00:00:00

abstract：:We have previously described a hamster sperm glycoprotein, P26h, which is implicated in the cascade of events occurring during the interaction between mature spermatozoa and the oocyte's zona pellucida. The P26h is acquired on the acrosomal cap of the spermatozoon during its maturation arising within the epididymis. L...

journal_title：Molecular reproduction and development

authors： Lamontagne N,Légaré C,Gaudreault C,Sullivan R

更新日期：2001-08-01 00:00:00

abstract：:cAMP response element binding protein (CREB)-binding protein (CBP) and p300 are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes. Current dogma would suggest that these transcriptional co-activators have similar mechanisms of transcription regulation. Studies of ...

journal_title：Molecular reproduction and development

authors： Kwok RP,Liu XT,Smith GD

更新日期：2006-07-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00

abstract：:This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...

journal_title：Molecular reproduction and development

authors： Shalgi R,Magnus A,Jones R,Phillips DM

更新日期：1994-03-01 00:00:00

abstract：:The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...

journal_title：Molecular reproduction and development

authors： Offenberg H,Barcroft LC,Caveney A,Viuff D,Thomsen PD,Watson AJ

更新日期：2000-12-01 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:Binding of neurotrophic ligands to rabbit spermatozoa was studied. Nicotinic cholinergic antagonists, [3H]alpha-bungarotoxin and [3H]dihydro-beta-erythroidine (DE), bound with high affinity to different sites in the tails of rabbit spermatozoa with the former binding to 10,207 sites/cell and the latter to 562 sites/ce...

journal_title：Molecular reproduction and development

authors： Young RJ,Laing JC

更新日期：1991-01-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

journal_title：Molecular reproduction and development

authors： Tian C,Liu J

更新日期：2013-09-01 00:00:00

abstract：:Oxygen consumption of preimplantation and early postimplantation mouse embryos has been measured using a novel noninvasive ultramicrofluorescence technique, based on an oil-soluble, nontoxic quaternary benzoid compound pyrene, whose fluorescence is quenched in the presence of oxygen. Pyruvate and glucose consumption, ...

journal_title：Molecular reproduction and development

authors： Houghton FD,Thompson JG,Kennedy CJ,Leese HJ

更新日期：1996-08-01 00:00:00

abstract：:The timing of the transition from maternal to zygotic control of development (MZT) and the initiation of transcription was studied in domestic cat embryos to determine if there is a temporal association between these phenomena and the development block observed in cat embryos fertilized in vitro. Embryos were derived ...

journal_title：Molecular reproduction and development

authors： Hoffert KA,Anderson GB,Wildt DE,Roth TL

更新日期：1997-10-01 00:00:00

abstract：:The cumulus cells that surround the mammalian oocyte become dispersed following the preovulatory surge of the pituitary gonadotropin, luteinizing hormone (LH). We have examined cumulus-oocyte complexes of PMSG-primed immature rats before and at 1, 2, 3, 4, 6, and 8 hr after injection of human chorionic gonadotropin (h...

journal_title：Molecular reproduction and development

authors： Phillips DM,Dekel N

更新日期：1991-03-01 00:00:00

abstract：:Ovorubin and PV2 are the major lipoglycocarotenoproteins present in the perivitellus of the freshwater snail eggs of Pomacea canaliculata, a rapidly expanding rice field pest. We have previously characterized these two particles regarding their lipid and protein compositions, their synthesis and tissular distribution,...

journal_title：Molecular reproduction and development

authors： Dreon MS,Heras H,Pollero RJ

更新日期：2004-07-01 00:00:00

abstract：:It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...

journal_title：Molecular reproduction and development

authors： Namiki Y,Endoh D,Kon Y

更新日期：2003-02-01 00:00:00

abstract：:Endometriosis is defined as endometrial tissue outside of the uterine cavity. The pathogenesis of this common disease remains poorly understood. However, the implantation and invasion of the viable cells from retrograde menstruation into the peritoneum is a widely accepted theory. To date, the mechanisms by which cell...

journal_title：Molecular reproduction and development

authors： Chen GT,Tai CT,Yeh LS,Yang TC,Tsai HD

更新日期：2002-07-01 00:00:00

abstract：:Following Northern analysis, GGT mRNA was found predominantly within the caput epididymides and kidney. The size of mRNAs for kidney, caput, corpus, and ductus deferens were 2.2, 2.3, 2.2, and 2.3 kb, respectively, whereas cauda showed a doublet of 2.2 and 2.3 kb. GGT transpeptidation and hydrolytic activity within ep...

journal_title：Molecular reproduction and development

authors： Hinton BT,Palladino MA,Mattmueller DR,Bard D,Good K

更新日期：1991-01-01 00:00:00

abstract：:Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...

journal_title：Molecular reproduction and development

authors： Wang R,Liu W,Liu X,Liu X,Tao H,Wu D,Zhao Y,Zou L

更新日期：2019-07-01 00:00:00

abstract：:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

journal_title：Molecular reproduction and development

authors： Mattmueller DR,Hinton BT

更新日期：1992-05-01 00:00:00

abstract：:Gamete membrane interaction is critical to initiate the development of a new organism. The signaling pathways governing this event, however, are poorly understood. In this report, we provide the first evidence that protein kinase C activity in mouse eggs plays a crucial role in the regulation of this process. Stimulat...

journal_title：Molecular reproduction and development

authors： Akabane H,Fan J,Zheng X,Zhu GZ

更新日期：2007-11-01 00:00:00