Abstract:
:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absence of androgen. We have previously demonstrated by two-dimensional (2-D) Western blot probing for clusterin that in epididymal fluid the amounts of clusterin were: caput greater than corpus greater than cauda. Luminal fluid from cauda epididymides was collected from control and orchiectomized rats (6 and 12 days) and orchiectomized animals that received testosterone implants. Equal volumes of fluid were analyzed by 2-D Western blot probing for clusterin. Following orchiectomy, there was an increase in clusterin in the luminal fluid after 6 days and maximal amount after 12 days compared with control cauda fluid. Orchiectomized animals which received testosterone treatment showed levels of clusterin comparable to that of controls. Serum clusterin was detected in fluid of orchiectomized animals with and without testosterone. Western blots of cauda sperm membrane extracts of control animals and orchiectomized animals treated with testosterone had a very low level of epididymal clusterin, whereas extracts collected from orchiectomized animals revealed high levels of clusterin. We suggest that, in the normal animal, clusterin is secreted into the lumen of the proximal epididymis where it binds to the sperm membrane. In the distal epididymis, clusterin dissociates from sperm and is processed (proteolysis/endocytosis). We hypothesize that, in the absence of androgen, the processing and regulation of clusterin is disrupted.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Mattmueller DR,Hinton BTdoi
10.1002/mrd.1080320112keywords:
subject
Has Abstractpub_date
1992-05-01 00:00:00pages
73-80issue
1eissn
1040-452Xissn
1098-2795journal_volume
32pub_type
杂志文章abstract::Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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更新日期:1996-05-01 00:00:00
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更新日期:1998-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-12-01 00:00:00
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21046
更新日期:2009-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00