Abstract：
:The reproductive tract environment at conception programs the developmental trajectory of the embryo, sets the course of pregnancy, and impacts offspring phenotype and health. Despite the fundamental importance of this stage of reproduction, the rate-limiting regulatory mechanisms operating locally to control fertility and fecundity are incompletely understood. Emerging studies highlight roles for microRNAs (miRNAs) in regulating reproductive and developmental processes and in modulating the quality and strength of the female immune response. Since endometrial receptivity and robust placentation require specific adaptation of the immune response, we hypothesize that miRNAs participate in establishing pregnancy through effects on key gene networks in immune cells. Our recent studies investigated miRNAs that are induced in the peri-conception environment, focusing on miRNAs that have immune-regulatory roles-particularly miR-223, miR-155, and miR-146a. Genetic mouse models deficient in individual miRNAs are proving informative in defining roles for these miRNAs in the generation and stabilization of regulatory T cells (Treg cells) that confer adaptive immune tolerance. Overlapping and redundant functions between miRNAs that target multiple genes, combined with multiple miRNAs targeting individual genes, indicate complex and sensitive regulatory networks. Although to date most data on miRNA regulation of reproductive events are from mice, conserved functions of miRNAs across species imply similar biological pathways operate in all mammals. Understanding the regulation and roles of miRNAs in the peri-conception immune response will advance our knowledge of how environmental determinants act at conception, and could have practical applications for animal breeding as well as human fertility.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Robertson SA,Zhang B,Chan H,Sharkey DJ,Barry SC,Fullston T,Schjenken JEdoi
10.1002/mrd.22823subject
Has Abstractpub_date
2017-09-01 00:00:00pages
914-925issue
9eissn
1040-452Xissn
1098-2795journal_volume
84pub_type
杂志文章,评审abstract：:Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200101)58:1<45::AID-MRD7>3.0.CO;
更新日期：2001-01-01 00:00:00
abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20495
更新日期：2007-02-01 00:00:00
abstract：:In this report we show that the first event of activation in the human oocyte, the Fertilization current (FC), is a slow transient outward current of 300 pA, which induces a gradual hyperpolarization of the plasma membrane from -20mV to -60mV, 60-120 min after insemination, followed by a repolarization to -20mV. Activ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380212
更新日期：1994-06-01 00:00:00
abstract：:The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/(SICI)1098-2795(199701)46:1<24::AID-MRD5>3
更新日期：1997-01-01 00:00:00
abstract：:Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080410415
更新日期：1995-08-01 00:00:00
abstract：:Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10234
更新日期：2003-04-01 00:00:00
abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199808)50:4<461::AID-MRD10
更新日期：1998-08-01 00:00:00
abstract：:Previous studies have shown that in adult male mice, expression of the meg1 gene is restricted to meiotic and early postmeiotic testicular germ cells. We have now analyzed the expression of meg1 during postnatal testicular development and the comparable meiotic stages in the female. The 0.75 kb transcript for meg1 beg...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080380104
更新日期：1994-05-01 00:00:00
abstract：:Biochemical and immunoelectron microscopic methods have been used to analyze the distribution of actin in boar spermatozoa and its state of aggregation before and after acrosome reaction. F-actin was detected on sperm head and tail by electron microscopy using an improved phalloidin probe: incubation with a fluorescei...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330114
更新日期：1992-09-01 00:00:00
abstract：:Many ascidians live in clumps and usually release sperm before the eggs. Consequently, eggs are often spawned into dense clouds of sperm. Because fertilization by more than a single sperm is lethal, ascidians have evolved at least two successive blocks to polyspermy: the rapid release of a glycosidase that inhibits sp...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199709)48:1<137::AID-MRD16
更新日期：1997-09-01 00:00:00
abstract：:Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22660
更新日期：2016-07-01 00:00:00
abstract：:This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20131
更新日期：2004-11-01 00:00:00
abstract：:A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20104
更新日期：2004-08-01 00:00:00
abstract：:In this study, we cloned and characterized the follicle stimulating hormone receptor (Fshr) and luteinizing hormone receptor (Lhr) cDNAs of pejerrey Odontesthes bonariensis, a species with temperature-dependent sex determination (TSD), and analyzed their expression in relation to Fshb and Lhb subunits during gonadogen...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21179
更新日期：2010-06-01 00:00:00
abstract：:Members of the Binder of SPerm (BSP) superfamily have been identified in both human and mouse epididymis. These proteins are known to bind sperm membrane and promote sperm capacitation. Studies suggest that BSPH2 might play a different role in sperm functions from its counterparts; however, the role of BSPH2 remains m...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23039
更新日期：2018-08-01 00:00:00
abstract：:The oviduct is a dynamic organ which modulates gamete physiology. Sperm-oviduct interaction provides the formation of a sperm storage reservoir and allows the selection of sperm with certain qualities in eutherian mammals. In sows, the oviductal sperm binding glycoprotein (SBG) has been proposed to be involved in sper...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20958
更新日期：2009-04-01 00:00:00
abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080360303
更新日期：1993-11-01 00:00:00
abstract：:Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200010)57:2<194::AID-MRD11>3.0.C
更新日期：2000-10-01 00:00:00
abstract：:Fibulin-1 is a secreted protein associated with elastic matrix fibres and basement membranes. It plays a role in stabilizing blood vessels and can also regulate cell motility and invasiveness. We studied the regulation of the fibulin-1 gene in the rat and human endometrium, an organ where cyclic tissue remodeling and ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20079
更新日期：2004-07-01 00:00:00
abstract：:Sry expression is essential for initiating male sex differentiation, and the expression occurs only during a restricted period in the developing gonad. It is thought that Sry is part of a pathway of genes that regulate sex determination. Although the interactions of several genes with Sry expression have been suggeste...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10278
更新日期：2003-04-01 00:00:00
abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20025
更新日期：2004-03-01 00:00:00
abstract：:A polymorphism in the igf2 gene of chickens was identified using NlaIII (GenBank accession number AF218827). In some embryos, the igf2 alleles were expressed monoallelically from either maternal or paternal alleles. These data demonstrate that genomic imprinting is not confined to mammalian vertebrates and suggest tha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200007)56:3<345::AID-MRD3>3.0.CO
更新日期：2000-07-01 00:00:00
abstract：:RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420313
更新日期：1995-11-01 00:00:00
abstract：:Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.22199
更新日期：2013-09-01 00:00:00
abstract：:cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420204
更新日期：1995-10-01 00:00:00
abstract：:A spontaneously established porcine granulosa cell line (PGC-2) was cloned through the continuous culturing of primary granulosa cells collected from equine chorionic gonadotropin (eCG)-treated prepubertal gilts. This established cell line has undergone approximately 100 passages and shows contact-inhibition of growth...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199611)45:3<299::AID-MRD6>
更新日期：1996-11-01 00:00:00
abstract：:Cumulus oocyte complexes (COCs) and cumulus oocyte complexes connected to a piece of the membrane granulosa (COCGs) were isolated from bovine antral follicles with a diameter of 2 to 8 mm. After culture of COCGs without gonadotrophic hormones for 22 hr approximately 50% of the oocytes were still in the germinal vesicl...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199610)45:2<218::AID-MRD15
更新日期：1996-10-01 00:00:00
abstract：:Oocyte maturation is dependent on a complex program of morphological, ultrastructural, and biochemical signaling events, and if disrupted could lead to decreased fertility and population decline. The in vitro sensitivity of amphibian oocytes and oocyte maturation to plant growth factor and widely used hormonal herbici...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10396
更新日期：2004-02-01 00:00:00
abstract：:Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22649
更新日期：2016-06-01 00:00:00
abstract：:Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/1098-2795(200009)57:1<88::AID-MRD12>3.0.CO
更新日期：2000-09-01 00:00:00
