Abstract:
:Sex-related growth rate differences in preimplantation mouse embryos were investigated. In experiment I, Day 3 embryos were recovered from reproductive tracts, classified according to developmental stage, and cultured for 24 hr in CZB medium containing glucose. Each embryo was then reclassified and stained for measurement of number of nuclei and finally sexed using the polymerase chain reaction. In experiment II, Day 4 embryos were recovered, classified, stained, and sexed as in experiment I immediately after recovery. Morphologically, there were no differences between the sexes in either of the experiments on Day 4. However, based on number of nuclei, the data showed that in vitro conditions support the development of male embryos to the blastocyst stage compared to female embryos. Furthermore, growth rate differences were observed in vivo on Day 3, as females compacted earlier than males. These results suggest that the increased cell proliferation in cultured male embryos is an artifact caused by the in vitro environment. The variation may be due to sex differences in embryonal energy metabolism during the preimplantation stage. The growth difference implies different in vitro requirements of male and female embryos.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Peippo J,Bredbacka Pdoi
10.1002/mrd.1080400108subject
Has Abstractpub_date
1995-01-01 00:00:00pages
56-61issue
1eissn
1040-452Xissn
1098-2795journal_volume
40pub_type
杂志文章abstract::Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23154
更新日期:2019-07-01 00:00:00
abstract::Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22158
更新日期:2013-03-01 00:00:00
abstract::In the rat, in response to blastocyst implantation, stromal cells of the endometrium proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression. This phenomenon appears to be due to an active process involving apoptosis. As there is ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20524
更新日期:2007-03-01 00:00:00
abstract::The objective of the present study was to investigate the ultrastructural consequences of vitrification of bovine oocytes at the metaphase II (MII) stage by the so-called "Open Pulled Straw" method. Oocytes were matured in vitro for 22 hr and cryopreserved by vitrification. After warming and additional 2 hr of culture...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200005)56:1<80::AID-MRD10>
更新日期:2000-05-01 00:00:00
abstract::Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410415
更新日期:1995-08-01 00:00:00
abstract::For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20705
更新日期:2007-10-01 00:00:00
abstract::The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22425
更新日期:2014-12-01 00:00:00
abstract::Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080270311
更新日期:1990-11-01 00:00:00
abstract::We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080010104
更新日期:1988-01-01 00:00:00
abstract::A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20104
更新日期:2004-08-01 00:00:00
abstract::The present study was designed to investigate the effect of vitrification on mitochondrial distribution, membrane potential (Deltapsi) and microtubule distribution in mouse 2-PN embryos, as well as to document the relationship between mitochondrial distribution and developmental ability of those embryos. Mitochondrial...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21064
更新日期:2009-11-01 00:00:00
abstract::The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199906)53:2<222::AID-MRD11
更新日期:1999-06-01 00:00:00
abstract::The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21328
更新日期:2011-07-01 00:00:00
abstract::A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with pr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199801)49:1<1::AID-MRD1>3.
更新日期:1998-01-01 00:00:00
abstract::Male infertility or subfertility is frequently associated with disruption of the hypothalamic-pituitary-testis axis events, like secondary hypogonadism. However, little is known how this condition affects the proteomic composition of the epididymal fluid. In the present study, we evaluated the proteomic changes in the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23408
更新日期:2020-09-01 00:00:00
abstract::Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20986
更新日期:2009-07-01 00:00:00
abstract::Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22020
更新日期:2012-04-01 00:00:00
abstract::We report use of an in vitro assay (Barbato et al., 1998: Biol Reprod 58:686-699) to assess binding ability of cauda epididymal mouse sperm to a surrogate zona pellucida and effect of a synthetic peptide (Amann et al., 1999: J Androl 20: 42-46) on fertilization ability in in vitro fertilization (IVF) tests. Sperm from...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<406::AID-MRD13>3.0.C
更新日期:2000-12-01 00:00:00
abstract::Endometriosis is regarded as a hormone-dependent disease. Current therapeutic approaches to treating this common gynecological disorder mainly depend on surgical and hormonal interventions, but the high rate of disease recurrence as well as the side effects related to such therapies make it difficult for patients to r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22493
更新日期:2015-06-01 00:00:00
abstract::XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22808
更新日期:2017-06-01 00:00:00
abstract::During implantation in mice, tissue inhibitor of matrix metalloproteinases-3 is believed to play a key role in inhibiting matrix metalloproteinase activity associated with embryo invasion and tissue remodeling. The first objective of this study was to quantitatively compare the steady-state mRNA levels of tissue inhib...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1018
更新日期:2001-06-01 00:00:00
abstract::The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standard parthenogenetic agent. The exposure of ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199710)48:2<292::AID-MRD18
更新日期:1997-10-01 00:00:00
abstract::We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20731
更新日期:2007-11-01 00:00:00
abstract::This report is part of a continuing study in which we employ monoclonal antibodies to membrane domains and internal organelles of rat spermatozoa in order to trace events during maturation, capacitation, fertilization, and early development. In the present study, we have used immunocytochemistry at the light and EM le...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370304
更新日期:1994-03-01 00:00:00
abstract::The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360303
更新日期:1993-11-01 00:00:00
abstract::Mouse zygotes or halves of zygotes, containing either a female or a male pronucleus, were fused with ovulated metaphase II oocytes. In 59.7% of the resulting hybrid cells, the pronuclei underwent premature chromosome condensation (PCC). In some of these heterokaryons the 2 pronuclei differed in the dynamics of condens...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340112
更新日期:1993-01-01 00:00:00
abstract::Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22418
更新日期:2014-10-01 00:00:00
abstract::Following Northern analysis, GGT mRNA was found predominantly within the caput epididymides and kidney. The size of mRNAs for kidney, caput, corpus, and ductus deferens were 2.2, 2.3, 2.2, and 2.3 kb, respectively, whereas cauda showed a doublet of 2.2 and 2.3 kb. GGT transpeptidation and hydrolytic activity within ep...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280107
更新日期:1991-01-01 00:00:00
abstract::This study was performed to investigate the effects of edaravone on hypoxia-induced trophoblast cell proliferation, apoptosis, and invasion. The trophoblast cell line HTR-8 (H8) was treated with cobalt chloride (CoCl2 ) with or without a 1-hr edaravone pretreatment, followed by assessment of intracellular reactive oxy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22652
更新日期:2016-07-01 00:00:00
abstract::Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(20010401)58:4<432::AID-MRD11>3.0
更新日期:2001-04-01 00:00:00