Protein kinase C is required for the disappearance of MPF upon artificial activation in mouse eggs.

abstract：:Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

journal_title：Molecular reproduction and development

authors： Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

更新日期：2000-11-01 00:00:00

abstract：:Glycogen synthase kinase-3 (GSK-3) is a highly conserved serine/threonine protein kinase implicated in diverse cellular processes. Activity of GSK-3 is essential for meiotic chromatin segregation in oocytes, yet expression and/or function of GSK-3 have not been reported in mammalian preimplantation embryos. Objectives...

journal_title：Molecular reproduction and development

authors： Acevedo N,Wang X,Dunn RL,Smith GD

更新日期：2007-02-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is the most common endocrinological pathology among women of reproductive age, and is characterized by abnormalities in ovarian angiogenesis, among other features. Consistent with this association, follicular fluid (FF) concentration and ovarian expression of vascular endothelial growt...

journal_title：Molecular reproduction and development

authors： Scotti L,Parborell F,Irusta G,De Zuñiga I,Bisioli C,Pettorossi H,Tesone M,Abramovich D

更新日期：2014-08-01 00:00:00

abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

journal_title：Molecular reproduction and development

authors： Xiong W,Wang Z,Shen C

更新日期：2019-08-01 00:00:00

abstract：:Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

journal_title：Molecular reproduction and development

authors： Manandhar G,Schatten G

更新日期：2000-08-01 00:00:00

abstract：:The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...

journal_title：Molecular reproduction and development

authors： Roy VK,Krishna A

更新日期：2011-07-01 00:00:00

abstract：:MAP kinase (MAPK) and its activator, MAP kinase kinase (MAPKK), are commonly activated by a variety of extracellular stimuli in mammalian cells and in the process of Xenopus oocyte maturation. In order to investigate the function of the MAPK cascade in oocyte maturation, we produced an anti-Xenopus MAPKK which specifi...

journal_title：Molecular reproduction and development

authors： Gotoh Y,Nishida E

更新日期：1995-12-01 00:00:00

abstract：:The ability of synthetic arginine-glycine-aspartic acid (RGD)-containing peptides to induce intracellular calcium transients similar to those observed at fertilization by spermatozoa in the bovine has been reported (Campbell et al., 2000: Biol Reprod 62:1702-1709; Sessions et al., 2006. Mol Reprod Dev). These results ...

journal_title：Molecular reproduction and development

authors： White KL,Passipieri M,Bunch TD,Campbell KD,Pate B

更新日期：2007-01-01 00:00:00

abstract：:An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...

journal_title：Molecular reproduction and development

authors： Ciarcia G,Paolucci M,Di Fiore MM

更新日期：1993-07-01 00:00:00

abstract：:An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...

journal_title：Molecular reproduction and development

authors： Tourte M,Besse C,Mounolou JC

更新日期：1991-12-01 00:00:00

abstract：:Mitochondrial metabolic capacity and DNA replication have both been shown to affect oocyte quality, but it is unclear which one is more critical. In this study, immature oocytes were treated with FCCP or ddC to independently inhibit the respective mitochondrial metabolic capacity or DNA replication of oocytes during i...

journal_title：Molecular reproduction and development

authors： Ge H,Tollner TL,Hu Z,Dai M,Li X,Guan H,Shan D,Zhang X,Lv J,Huang C,Dong Q

更新日期：2012-06-01 00:00:00

abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

journal_title：Molecular reproduction and development

authors： Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

更新日期：2007-09-01 00:00:00

abstract：:Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of sh...

journal_title：Molecular reproduction and development

authors： Yuan YG,Mesalam A,Song SH,Lee KL,Xu L,Joo MD,Kong IK

更新日期：2020-10-01 00:00:00

abstract：:The Src family kinase (SFK) is important in normal cell cycle control. However, its role in meiotic maturation in mammalian has not been examined. We used confocal microscope immunofluorescence to examine the in vitro dynamics of the subcellular distribution of SFK during the mouse oocyte meiotic maturation and furthe...

journal_title：Molecular reproduction and development

authors： Zheng KG,Meng XQ,Yang Y,Yu YS,Liu DC,Li YL

更新日期：2007-01-01 00:00:00

abstract：:Changes in the level of transcriptional activity in 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients were pulse labelled with 3H-uridine during cultivation in vitro, embryos were then fixed and processed for autoradiography and electron micros...

journal_title：Molecular reproduction and development

authors： Kanka J,Hozák P,Heyman Y,Chesné P,Degrolard J,Renard JP,Fléchon JE

更新日期：1996-02-01 00:00:00

abstract：:We have previously described a hamster sperm glycoprotein, P26h, which is implicated in the cascade of events occurring during the interaction between mature spermatozoa and the oocyte's zona pellucida. The P26h is acquired on the acrosomal cap of the spermatozoon during its maturation arising within the epididymis. L...

journal_title：Molecular reproduction and development

authors： Lamontagne N,Légaré C,Gaudreault C,Sullivan R

更新日期：2001-08-01 00:00:00

abstract：:The developmental competence of bovine follicular oocytes that had been meiotically arrested with the phosphokinase inhibitor 6-dimethylaminopurine (6-DMAP) was studied. After 24 h in vitro culture with 2 mM 6-DMAP, 85 +/- 12% of the oocytes were at the germinal vesicle stage compared to 97 +/- 3% at the start of cult...

journal_title：Molecular reproduction and development

authors： Avery B,Hay-Schmidt A,Hyttel P,Greve T

更新日期：1998-07-01 00:00:00

abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...

journal_title：Molecular reproduction and development

authors： Foster JA,Gerton GL

更新日期：1996-06-01 00:00:00

abstract：:Myeloid cell leukemia-1 (MCL1), an anti-apoptotic member of the BCL2 family, is expressed abundantly in the testis. Previous characterization revealed that MCL1 is expressed exclusively in the Leydig cells in the mouse testis, yet what it does in these cells remains unknown. We therefore analyzed testosterone biosynth...

journal_title：Molecular reproduction and development

authors： Guang-Yu L,Hai-Yan L,Ji-Hong L,Yun-Cong M,Xue-Lian D,Chun-Yu L,Wen-Yong S

更新日期：2016-03-01 00:00:00

abstract：:Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...

journal_title：Molecular reproduction and development

authors： Moro CF,Consonni SR,Rosa RG,Nascimento MA,Joazeiro PP

更新日期：2012-04-01 00:00:00

abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...

journal_title：Molecular reproduction and development

authors： Ijiri TW,Vadnais ML,Lin AM,Huang AP,Cao W,Merdiushev T,Gerton GL

更新日期：2013-10-01 00:00:00

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 µm in diameter) or full-gr...

journal_title：Molecular reproduction and development

authors： Kyogoku H,Ogushi S,Miyano T,Fulka J Jr

更新日期：2011-06-01 00:00:00

abstract：:There are always more evidences indicating that 17beta-estradiol (E2) is to be necessary for normal male fertility. Here we report the expression of the most ubiquitously expressed member of the akt family of genes, akt1, in the lizard (Podarcis s. sicula) testis. We have used a nonmammalian vertebrate model (the liza...

journal_title：Molecular reproduction and development

authors： Russo M,Troncone G,Guarino FM,Angelini F,Chieffi P

更新日期：2005-05-01 00:00:00

abstract：:The developmental pattern of expression of the Strongylocentrotus purpuratus CyIIIb actin gene was determined by RNA blot hybridizations carried out with a gene-specific probe and total embryonic RNA isolated from various stages of development. The results indicate that the CyIIIb mRNA is not detected in the maternal ...

journal_title：Molecular reproduction and development

authors： Flytzanis CN,Bogosian EA,Niemeyer CC

更新日期：1989-01-01 00:00:00

abstract：:Acrosin is thought to fulfill several different roles in fertilization including that of a serine protease and in secondary zona pellucida (ZP) binding. However, acrosin's importance as a fertilization protein has been questioned. Especially since it was discovered that acrosin knockout mice are fertile. In this study...

journal_title：Molecular reproduction and development

authors： Raterman D,Springer MS

更新日期：2008-07-01 00:00:00

abstract：:It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...

journal_title：Molecular reproduction and development

authors： Baumann CG,Morris DG,Sreenan JM,Leese HJ

更新日期：2007-10-01 00:00:00

abstract：:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential...

journal_title：Molecular reproduction and development

authors： McBride CE,Fayrer-Hosken RA,Srivastava PN,Brackett BG

更新日期：1990-05-01 00:00:00

abstract：:We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the ma...

journal_title：Molecular reproduction and development

authors： Dalbiès-Tran R,Papillier P,Pennetier S,Uzbekova S,Monget P

更新日期：2005-08-01 00:00:00

abstract：:The presence and possible role of products of nuclear (c-fos and c-jun) and c-ras proto-oncogenes were investigated in preimplantation embryonic development in mice. Polyclonal antibodies to c-fos or c-jun proto-oncogene products did not affect development of in vitro-cultured embryos from two-cell to morula or from m...

journal_title：Molecular reproduction and development

authors： Ahmad K,Naz RK

更新日期：1993-11-01 00:00:00