Abstract：
:In mammals, the nucleolus of full-grown oocyte is essential for embryonic development but not for oocyte maturation. In our study, the role of the growing oocyte nucleolus in oocyte maturation was examined by nucleolus removal and/or transfer into previously enucleolated, growing (around 100 µm in diameter) or full-grown (120 µm) pig oocytes. In the first experiment, the nucleoli were aspirated from growing oocytes whose nucleoli had been compacted by actinomycin D treatment, and the enucleolated oocytes were matured in vitro. Most of non-treated or actinomycin D-treated oocytes did not undergo germinal vesicle breakdown (GVBD; 13% and 12%, respectively). However, the GVBD rate of enucleolated, growing oocytes significantly increased to 46%. The low GVBD rate of enucleolated, growing oocytes was restored again by the re-injection of nucleoli from growing oocytes (23%), but not when nucleoli from full-grown oocytes were re-injected into enucleolated, growing oocytes (49%). When enucleolated, full-grown oocytes were injected with nucleoli from growing or full-grown oocytes, the nucleolus in the germinal vesicle was reassembled (73% and 60%, respectively). After maturation, the enucleolated, full-grown oocytes injected with nucleoli from full-grown oocytes matured to metaphase II (56%), whereas injection with growing-oocyte nucleoli reduced this maturation to 21%. These results suggest that the growing-oocyte nucleolus is involved in the oocyte's meiotic arrest, and that the full-grown oocyte nucleolus has lost the ability.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Kyogoku H,Ogushi S,Miyano T,Fulka J Jrdoi
10.1002/mrd.21320subject
Has Abstractpub_date
2011-06-01 00:00:00pages
426-35issue
6eissn
1040-452Xissn
1098-2795journal_volume
78pub_type
杂志文章abstract：:Sperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080280410
更新日期：1991-04-01 00:00:00
abstract：:The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22498
更新日期：2015-06-01 00:00:00
abstract：:cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22329
更新日期：2014-07-01 00:00:00
abstract：:The lack of a paternal genome in parthenogenetic embryos clearly limits their postimplantation development, but apparently not their preimplantation development, since morphologically normal blastocysts can be formed. The cleavage rate of these embryos during the preimplantation period gives a better indication of the...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080310406
更新日期：1992-04-01 00:00:00
abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20214
更新日期：2005-03-01 00:00:00
abstract：:Five experiments, utilizing 3741 embryos produced in vitro, were designed to test the effects of Eagle's nonessential amino acids, and combinations of Eagle's essential amino acids and the RNA polymerase inhibitor alpha-amanitin on the development of preimplantation bovine embryos in a modified protein-free KSOM mediu...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199703)46:3<278::AID-MRD6>
更新日期：1997-03-01 00:00:00
abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199705)47:1<19::AID-MRD3>3
更新日期：1997-05-01 00:00:00
abstract：:The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20207
更新日期：2005-03-01 00:00:00
abstract：:Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20523
更新日期：2007-02-01 00:00:00
abstract：:The objective of this study was to use atomic force microscopy (AFM), with submicron resolution, for morphophologic and morphometric analyses of acrosome intact and acrosome-reacted human sperm heads. A mixed population of acrosome intact and reacted sperm was produced by treating capacitated sperm with A23187, which ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10195
更新日期：2002-12-01 00:00:00
abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199810)51:2<130::AID-MRD2>
更新日期：1998-10-01 00:00:00
abstract：:Using alpha and beta spectrin mammalian antibodies on Western blotting, we demonstrated that lizard ovarian follicles contain two isoforms of alpha spectrin, Mr 94 and 134 kDa, and a 230 kDa beta spectrin, and that their pattern modifies in relation to pyriform cell differentiation. In fact, a positive immunoreaction ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20008
更新日期：2004-01-01 00:00:00
abstract：:We have previously described a hamster sperm glycoprotein, P26h, which is implicated in the cascade of events occurring during the interaction between mature spermatozoa and the oocyte's zona pellucida. The P26h is acquired on the acrosomal cap of the spermatozoon during its maturation arising within the epididymis. L...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1050
更新日期：2001-08-01 00:00:00
abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199912)54:4<396::AID-MRD10
更新日期：1999-12-01 00:00:00
abstract：:The developmental competence of bovine follicular oocytes that had been meiotically arrested with the phosphokinase inhibitor 6-dimethylaminopurine (6-DMAP) was studied. After 24 h in vitro culture with 2 mM 6-DMAP, 85 +/- 12% of the oocytes were at the germinal vesicle stage compared to 97 +/- 3% at the start of cult...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199807)50:3<334::AID-MRD10
更新日期：1998-07-01 00:00:00
abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10143
更新日期：2002-08-01 00:00:00
abstract：:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080260106
更新日期：1990-05-01 00:00:00
abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10303
更新日期：2003-07-01 00:00:00
abstract：:Selection of transgenic embryos prior to embryo transfer is a means to increase the efficiency of transgenic livestock production. Among transgenic reporters, cytoplasmic expression of green fluorescent protein (GFP) has features that make it ideal for transgenic embryo selection. The primary objective of this study w...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>
更新日期：1999-04-01 00:00:00
abstract：:The present study was designed to determine the effects of vitrification on promoter methylation and the expression levels of pluripotency and differentiation genes in mouse blastocysts. Promoter region CpG methylation patterns and the expression levels of octamer-binding transcription factor (Oct4), Nanog homeobox (N...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22052
更新日期：2012-07-01 00:00:00
abstract：:The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22442
更新日期：2015-01-01 00:00:00
abstract：:During bovine embryogenesis, bovine growth hormone (bGH) contributes to proliferation, differentiation, and modulation of embryo metabolism. Pituitary-specific transcription factor-1 (Pit-1) is a transcription factor that binds to promoters of GH, prolactin (PRL), and thyroid-stimulating hormone-beta (TSHbeta) encodin...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10237
更新日期：2003-03-01 00:00:00
abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080340116
更新日期：1993-01-01 00:00:00
abstract：:Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of sh...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23417
更新日期：2020-10-01 00:00:00
abstract：:Although the epididymal environment promotes the maturation and survival of spermatozoa, not all spermatozoa remain viable during passage through the epididymis. Does the epididymis has a protective mechanism(s) to segregate the viable sperm from defective spermatozoa? Previously, we identified 260/280 kDa oligomers (...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.23438
更新日期：2020-12-01 00:00:00
abstract：:Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10065
更新日期：2002-05-01 00:00:00
abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199811)51:3<295::AID-MRD9>
更新日期：1998-11-01 00:00:00
abstract：:The first indication that the insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is developmentally regulated came from studies of the serum form of the receptor in the rat. By immunoblotting, the circulating form of the receptor, which was 10 kDa smaller than the tissue receptor, was high in 19 ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1080350415
更新日期：1993-08-01 00:00:00
abstract：:As a means of determining whether ovarian follicular fluid reaches the functional sperm reservoir in the caudal isthmus of the Fallopian tube shortly after ovulation, 0.01-0.02 ml aliquots of whole or steroid-free follicular fluid were introduced into the distal extremity of the isthmus within 1 hr before ovulation. E...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199911)54:3<283::AID-MRD9>
更新日期：1999-11-01 00:00:00
abstract：:Oocyte meiosis is arrested at prophase I by factors secreted from surrounding somatic cells after oocytes acquire meiotic competence at an early antral stage, and meiosis resumes in preovulatory follicles as a result of the luteinizing hormone (LH) surge. Recently, signaling by C-type natriuretic peptide (CNP) through...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22114
更新日期：2012-11-01 00:00:00
