Abstract：
:Many studies have shown a correlation between transferrin (Tf) concentration and sperm yield in several mammalian species. We have used transgenic mice expressing human Tf (hTf) to investigate if overexpression of Tf increases the efficiency of mouse spermatogenesis. We demonstrated that a 36% increase of Tf does not ameliorate the efficiency of mouse spermatogenesis but on the contrary resulted in a 36% decrease of testis sperm reserves. Tf overexpression had no effect on testicular determination and development, however testicular function of these transgenic mice was affected in an age-dependent manner. At 16 months of age, testicular and epididymal weights were significantly reduced. While spermatogenesis was qualitatively normal, testicular functions were perturbed. In fact, testosterone rate after human chorionic gonadotropin (hCG) stimulation was lower in Tf overexpressing mice. Intratesticular concentration of estradiol-17beta was increased and fluid accumulation after ligation of rete testis was more abundant in these transgenic mice. Surprisingly, we found that endogenous Tf levels were also increased in Tf overexpressing mice and we demonstrated for the first time that Tf may serve to upregulate its own expression in testis. Collectively, our data show that Tf overexpression has negative effects on testicular function and that Tf levels require strict regulation in the testis.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Lécureuil C,Staub C,Fouchécourt S,Maurel MC,Fontaine I,Martinat N,Gauthier C,Daudignon A,Delaleu B,Sow A,Jégou B,Guillou Fdoi
10.1002/mrd.20523subject
Has Abstractpub_date
2007-02-01 00:00:00pages
197-206issue
2eissn
1040-452Xissn
1098-2795journal_volume
74pub_type
杂志文章abstract：:Among Sicilian stick insects, two hybridogenetic complexes have been discovered: Bacillus rossius-grandii benazzii and B. rossius-grandii grandii, which also produce androgenetic offspring. The egg maturation of the former is analyzed here through DAPI fluorometry, which, besides the assessment of the meiotic stages, ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080330302
更新日期：1992-11-01 00:00:00
abstract：:Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ line specification during embryogenesis is conceptually the same as the specification of any som...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.21091
更新日期：2010-01-01 00:00:00
abstract：:Successful fertilization in mammals depends on the sperm's ability to initiate intracellular Ca2+ oscillations in the egg, a phenomenon that is elicited by Sperm-oocyte activating factors (SOAFs), whose quantitative and/or qualitative defect might result in fertilization failure. One such proposed factor is Post-acros...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22950
更新日期：2018-02-01 00:00:00
abstract：:Triosephosphate isomerase 1 (TPI1) is a member of the glycolytic pathway, which is a critical source of energy for motility in mouse sperm. By immunoblotting, we detected two male, germ line-specific TPI1 bands (Mr 33,400 and 30,800) as well as the somatic-type band (Mr 27,700). Although all three bands were observed ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22217
更新日期：2013-10-01 00:00:00
abstract：:There are always more evidences indicating that 17beta-estradiol (E2) is to be necessary for normal male fertility. Here we report the expression of the most ubiquitously expressed member of the akt family of genes, akt1, in the lizard (Podarcis s. sicula) testis. We have used a nonmammalian vertebrate model (the liza...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20284
更新日期：2005-05-01 00:00:00
abstract：:It has been reported that the MRL/MpJ mouse strain shows several unique phenotypes, including rapid wound healing, inherent collagen disease, heat shock-resistant spermatocytes, and metaphase-specific apoptosis (Msa) in the testis. In the present study, we found the genetic mutation associated with Msa by chromosomal ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10208
更新日期：2003-02-01 00:00:00
abstract：:We have investigated the origin of the sperm motility inhibitor (SPMI) from boar seminal plasma. SPMI was measured by its capacity to inhibit the motility of demembranated spermatozoa and by an enzyme-linked immunosorbent assay (ELISA). Among the various reproductive and now reproductive tissues and fluids tested, onl...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080360411
更新日期：1993-12-01 00:00:00
abstract：:There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20712
更新日期：2008-01-01 00:00:00
abstract：:Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20512
更新日期：2006-08-01 00:00:00
abstract：:In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199606)44:2<141::AID-MRD1>
更新日期：1996-06-01 00:00:00
abstract：:The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20697
更新日期：2007-10-01 00:00:00
abstract：:Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199604)43:4<484::AID-MRD11
更新日期：1996-04-01 00:00:00
abstract：:The IGFs (IGF-I and IGF-II) are essential for normal mammalian growth and development. Their actions are mediated primarily by their interactions with the type I IGF receptor (IGF-I receptor), a transmembrane tyrosine kinase. The ligands and the IGF-I receptor are structurally related to insulin and to the insulin rec...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.1080350403
更新日期：1993-08-01 00:00:00
abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/(SICI)1098-2795(199811)51:3<295::AID-MRD9>
更新日期：1998-11-01 00:00:00
abstract：:Development of female gonads in the chicken is asymmetric. This asymmetry affects gene expression, morphology, and germ cell development; consequently only the left ovary develops into a functional organ, whereas the right ovary remains vestigial. In males, on the other hand, both gonads develop into functional testes...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22516
更新日期：2015-10-01 00:00:00
abstract：:The present study examined the effect of elevated Ca(2+) concentration in fusion/activation medium on the fusion and development of fetal fibroblast nuclear transfer (NT) porcine embryos. Frozen-thawed and serum starved fetal fibroblasts were transferred into the perivitelline space of enucleated oocytes. Cell fusion ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10110
更新日期：2002-04-01 00:00:00
abstract：:Proteins synthesized by DDK mice embryos were analyzed by 2D electrophoresis and a new egg-specific polypeptide, D14, was identified. The protein is characterized by its high rate of synthesis and electrophoretic properties (MW 36,500, pl greater than 8). The synthesis of D14 is strictly developmentally regulated: sta...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080280303
更新日期：1991-03-01 00:00:00
abstract：:The localization of fibrillarin and nucleolin in the nuclei of mouse two-cell, four-cell, and eight-cell embryos has been studied using immunofluorescent staining with specific antibodies. In all of these cleavage stages, both antigens were associated exclusively with the peripheral region of the nucleolus precursor b...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080400306
更新日期：1995-03-01 00:00:00
abstract：:A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20401
更新日期：2006-05-01 00:00:00
abstract：:The objective of the present study was to determine whether glucocorticoid (GC) and its receptor (GC-R) are expressed in the porcine corpus luteum (CL), and whether GC influences porcine luteal hormone production. The gene expressions of 11beta-hydroxysteroid dehydrogenase type 1 (11-HSD1), type 2 (11-HSD2), GC-R, and...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20833
更新日期：2008-05-01 00:00:00
abstract：:The presence of two ras-related proteins (22 and 23 kDa) was demonstrated in Xenopus embryonic extracts by selective immunoprecipitation using anti-ras monoclonal antibodies 142-24E05 and Y13-259. We further describe the cytological effects of the microinjection of anti-ras monoclonal antibody Y13-259 into early cleav...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080250403
更新日期：1990-04-01 00:00:00
abstract：:It has been proposed that the viability of early mammalian embryos is associated with a metabolism that is "quiet" rather than "active" (Leese HJ, 2002:BioEssays 24:845-849). The data on which this hypothesis was based were largely drawn from measurements on the depletion and appearance of amino acids from the culture...
journal_title：Molecular reproduction and development
pub_type： 杂志文章,评审
doi：10.1002/mrd.20604
更新日期：2007-10-01 00:00:00
abstract：:Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) ha...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20038
更新日期：2004-04-01 00:00:00
abstract：:Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.1080420303
更新日期：1995-11-01 00:00:00
abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20182
更新日期：2005-01-01 00:00:00
abstract：:Expression of ovine interferon-tau (oIFNtau) genes, essential for the maternal recognition of pregnancy in ruminant ungulates, is restricted to the trophoblast and is not detected in any other cell types or tissues. Substantial secretion of oIFNtau starts on day 12-13 of pregnancy (day 0 = day of estrus), reaches the ...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20002
更新日期：2004-04-01 00:00:00
abstract：:Fertile offspring from somatic cell nuclear transfer (SCNT) is the goal of most cloning laboratories. For this process to be successful, a number of events must occur correctly. First the donor nucleus must be in a state that is amenable to remodeling and subsequent genomic reprogramming. The nucleus must be introduce...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.21242
更新日期：2010-12-01 00:00:00
abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.10143
更新日期：2002-08-01 00:00:00
abstract：:In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.22922
更新日期：2017-12-01 00:00:00
abstract：:Factors affecting the efficiency of producing transgenic rats by intracytoplasmic sperm injection (ICSI)-mediated DNA transfer were investigated. Epididymal spermatozoa from Sprague-Dawley (SD) rats were sonicated and/or frozen-thawed for cutting the tail and membrane disruption. The sperm heads were exposed for 1 min...
journal_title：Molecular reproduction and development
pub_type： 杂志文章
doi：10.1002/mrd.20223
更新日期：2005-04-01 00:00:00
