Genome exclusion and gametic DAPI-DNA content in the hybridogenetic Bacillus rossius-grandii benazzii complex (Insecta Phasmatodea).

abstract：:In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...

journal_title：Molecular reproduction and development

authors： Tesfaye D,Lonergan P,Hoelker M,Rings F,Nganvongpanit K,Havlicek V,Besenfelder U,Jennen D,Tholen E,Schellander K

更新日期：2007-08-01 00:00:00

abstract：:Rainbow trout (Oncorhynchus mykiss) oocytes were incubated for 3 hr in ovarian fluid alone (CC), or cortisol-enriched ovarian fluid [100 or 1,000 ng ml(-1) (CL and CH, respectively)], after which they were fertilized; the growth and development of the embryos reared from these oocytes was monitored until first feed, a...

journal_title：Molecular reproduction and development

authors： Li M,Bureau DP,King WA,Leatherland JF

更新日期：2010-10-01 00:00:00

abstract：:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...

journal_title：Molecular reproduction and development

authors： Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM

更新日期：1995-12-01 00:00:00

abstract：:Forward-motility-stimulating factor (FMSF) is a protein, originally purified from bubaline serum, that promotes progressive motility of mature spermatozoa. FMSF binds to sperm surface receptors and activates transmembrane adenylyl cyclase (tmAC), causing a rise in intracellular cyclic AMP level ([cAMP]i) and subsequen...

journal_title：Molecular reproduction and development

authors： Dey S,Roy D,Majumder GC,Mukherjee B,Bhattacharyya D

更新日期：2015-12-01 00:00:00

abstract：:The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Ferrière F,Guiguen Y,Bailhache T,Breton B,Lareyre JJ

更新日期：2001-05-01 00:00:00

abstract：:The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...

journal_title：Molecular reproduction and development

authors： Munsie M,Schlatt S,deKretser DM,Loveland KL

更新日期：1997-05-01 00:00:00

abstract：:Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...

journal_title：Molecular reproduction and development

authors： Ergenc AF,Li MW,Toner M,Biggers JD,Lloyd KC,Olgac N

更新日期：2008-12-01 00:00:00

abstract：:Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not...

journal_title：Molecular reproduction and development

authors： Moody MA,Cardona C,Simpson AJ,Smith TT,Travis AJ,Ostermeier GC

更新日期：2017-05-01 00:00:00

abstract：:To study self-renewal, genetic modification, and differentiation of avian spermatogonial stem cells (SSCs), we isolated chicken SSCs from fetal testes on the 16th hatching day via enzyme digestion, and then cultured the SSCs over 2 months after purification in vitro. SSCs were identified by alkaline phosphatase staini...

journal_title：Molecular reproduction and development

authors： Yu F,Ding LJ,Sun GB,Sun PX,He XH,Ni LG,Li BC

更新日期：2010-04-01 00:00:00

abstract：:So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...

journal_title：Molecular reproduction and development

authors： Ola SI,Ai JS,Liu JH,Wang Q,Wang ZB,Chen DY,Sun QY

更新日期：2008-01-01 00:00:00

abstract：:We have previously shown that each of the metalloendoprotease (MEP) inhibitors phosphoramidon, diethylenetriaminepentaacetic acid, and carbobenzoxy-L-phenylalanine, when present only during the human sperm acrosome reaction (AR), will not inhibit the AR or sperm motility but will decrease the number of sperm that pene...

journal_title：Molecular reproduction and development

authors： Díaz-Pérez E,Meizel S

更新日期：1992-02-01 00:00:00

abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...

journal_title：Molecular reproduction and development

authors： Holden CA,Hyne RV,Sathananthan AH,Trounson AO

更新日期：1990-03-01 00:00:00

abstract：:Human ejaculate spermatozoa contain multiple mRNA species carried over from earlier stages in spermatogenesis. To date, gene-specific RT-PCR or in situ hybridization has detected transcripts for beta-actin, heat shock proteins (HSP) 70 and 90, protamines (PRM) 1 and 2, transition protein (TNp) 2, HLA II, beta-integrin...

journal_title：Molecular reproduction and development

authors： Miller D

更新日期：2000-06-01 00:00:00

abstract：:Acrosomal reaction is an essential prerequisite to fertilization. The changes in lipid composition of sperm membranes cause fusion of the plasma and outer acrosomal membranes that results in the exocytosis of acrosomal contents. We report that both bull and rabbit spermatozoa contain a phosphatidylcholine-specific pho...

journal_title：Molecular reproduction and development

authors： Hinkovska-Galchev V,Srivastava PN

更新日期：1992-11-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:Reverse transcriptase polymerase chain reaction (RT-PCR) analysis shows that Sry mRNA is expressed in male fetal urogenital ridges from 12.5 day p.c. embryos, but not in enriched populations of primordial germ cells from the same embryos, indicating that Sry is expressed in the somatic cells of the embryonal gonad at ...

journal_title：Molecular reproduction and development

authors： Rossi P,Dolci S,Albanesi C,Grimaldi P,Geremia R

更新日期：1993-04-01 00:00:00

abstract：:Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...

journal_title：Molecular reproduction and development

authors： Raz T,Skutelsky E,Amihai D,Hammel I,Shalgi R

更新日期：1998-11-01 00:00:00

abstract：:We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...

journal_title：Molecular reproduction and development

authors： Raychoudhury SS,Blackshaw AW,Irving MG

更新日期：1993-06-01 00:00:00

abstract：:PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...

journal_title：Molecular reproduction and development

authors： Massabbal E,Parveen S,Weisoly DL,Nelson DM,Smith SD,Fant M

更新日期：2005-07-01 00:00:00

abstract：:Lactate produced by glycolysis in Sertoli cells (SCs) is the main energy substrate for developing germ cells and plays a vital role in spermatogenesis. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. We have previously shown that hyperthermia (43°C, 30 min) pro...

journal_title：Molecular reproduction and development

authors： Hu Y,Deng J,Tian K,Yang WR,Luo NJ,Lian Y,Gan L,Tang XY,Luo HY,Zhang JJ,Wang XZ

更新日期：2019-11-01 00:00:00

abstract：:In fish, growth hormone (GH), prolactin (PRL) and somatolactin (SL) are three major peptide hormones produced in the pituitary gland. Using reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis of the amplified products, the expression of GH, PRL, and SL genes were determined during the e...

journal_title：Molecular reproduction and development

authors： Yang BY,Greene M,Chen TT

更新日期：1999-06-01 00:00:00

abstract：:In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...

journal_title：Molecular reproduction and development

authors： Gu W,Kwon YK,Hecht NB

更新日期：1996-06-01 00:00:00

abstract：:A multicolor procedure employing fluorescence in situ hybridization is described for detecting chromosomal domains and germinal aneuploidy in late-step spermatids in mice using DNA probes specific for repetitive sequences near the centromeres of chromosomes 8 and X. These probes were nick-translated with biotin- or di...

journal_title：Molecular reproduction and development

authors： Wyrobek A,Lowe X,Pinkel D,Bishop J

更新日期：1995-02-01 00:00:00

abstract：:Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

journal_title：Molecular reproduction and development

authors： Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

更新日期：2018-12-01 00:00:00

abstract：:The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger ...

journal_title：Molecular reproduction and development

authors： Cohen J,Feldberg D

更新日期：1991-09-01 00:00:00

abstract：:The distribution of microtubules was studied during fertilization of the rabbit oocyte by immunofluorescence microscopy after staining with an anti-alpha-tubulin antibody. In ovulated oocytes, microtubules were found exclusively in the meiotic spindle. At fertilization, the paternal centrosome generated sperm astral m...

journal_title：Molecular reproduction and development

authors： Yllera-Fernández MM,Crozet N,Ahmed-Ali M

更新日期：1992-07-01 00:00:00

abstract：:The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

journal_title：Molecular reproduction and development

authors： Manning KR

更新日期：2009-10-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...

journal_title：Molecular reproduction and development

authors： Lane M,Bavister BD

更新日期：1999-12-01 00:00:00

abstract：:Mouse oocyte maturation inhibitory factors, on the basis of inhibitory activity of spontaneous germinal vesicle breakdown (GVBD) of denuded mouse oocytes in culture, were extracted and partially purified by reversed-phase resin adsorption and Sephadex G-100 and G-50 column chromatographies from the urine of pregnant w...

journal_title：Molecular reproduction and development

authors： Sakakibara R,Sakai K,Sakurai Y,Kohnoura T,Ishiguro M

更新日期：1993-01-01 00:00:00