Abstract:
:Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An important and remaining problem with this technique, however, is that it requires small amounts of mercury to stabilize the pipette tip when piezoelectric force pulses are applied. To eliminate this problem we developed and tested a completely different and mercury-free technology, called the "Ros-Drill" (rotationally oscillating drill). The technique uses microprocessor-controlled rotational oscillations on a spiked micropipette without mercury or piezo. Preliminary experimental results show that this new microinjection technology gives high survival rate (>70% of the injected oocytes) and fertilization rate (>80% of the survived oocytes), and blastocyst formation rates in early trials (approximately 50% of the survived oocytes). Blastocysts created by Ros-Drill ICSI were transferred into the uteruses of pseudopregnant surrogate mothers and healthy pups were born and weaned. The Ros-Drill ICSI technique is automated and therefore; it requires a very short preliminary training for the specialists, as evidenced in many successful biological trials. These advantages of Ros-Drill ICSI over conventional and piezo-assisted ICSI are clearly demonstrated and it appears to have resolved an important problem in reproductive biology.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Ergenc AF,Li MW,Toner M,Biggers JD,Lloyd KC,Olgac Ndoi
10.1002/mrd.20919subject
Has Abstractpub_date
2008-12-01 00:00:00pages
1744-51issue
12eissn
1040-452Xissn
1098-2795journal_volume
75pub_type
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journal_title:Molecular reproduction and development
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更新日期:2000-06-01 00:00:00
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更新日期:1997-01-01 00:00:00
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journal_title:Molecular reproduction and development
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