Analysis and significance of messenger RNA in human ejaculated spermatozoa.

abstract：:The mRNAs of the nuclear encoded genes, ornithine decarboxylase (ODCase) and poly(ADP)ribose polymerase (PADPRP), and the mitochondrial encoded genes, cytochrome oxidase I and II (COI and COII) and ATPase 6, are differentially expressed during spermatogenesis (Alcivar et al., 1989: Biol Reprod 41:1133; 1989: Dev Biol ...

journal_title：Molecular reproduction and development

authors： Alcivar-Warren A,Trasler JM,Awoniyi CA,Zirkin BR,Hecht NB

更新日期：1996-03-01 00:00:00

abstract：:The uterus and the placenta synthesize insulin-like growth factors (IGFs) and insulin-like binding proteins (IGFBPs). These growth factors are implicated in processes of proliferation and differentiation that occur in the uterus. To determine the patterns of expression of IGFs during rat pregnancy we used in situ hybr...

journal_title：Molecular reproduction and development

authors： Correia-da-Silva G,Bell SC,Pringle JH,Teixeira N

更新日期：1999-07-01 00:00:00

abstract：:Telomeres are specialized natural ends of eukaryotic chromosomes that, contrary to the ends of broken chromosomes, are stable and do not fuse with the ends of other chromosomes. In addition, telomeres protect chromosomal ends from degradation, facilitate completion of chromosomal DNA replication, and contribute to chr...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2000-09-01 00:00:00

abstract：:In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells du...

journal_title：Molecular reproduction and development

authors： Golan R,Weissenberg R,Oschry Y,Shochat L,Lewin LM

更新日期：2000-02-01 00:00:00

abstract：:Annexins are a family of Ca(2+)-binding proteins involved in the exocytotic process. The presence and the role of annexins in mammalian spermatozoa have not been well established. Two annexin-like proteins were obtained from guinea pig testis, a doublet of Mr 31-33 kD (p31/33) and a protein of Mr 50 kD (p50). Both pro...

journal_title：Molecular reproduction and development

authors： Othón Hernández E,Roa-Espitia AL,Cruz Trejo J,Mújica A

更新日期：1996-03-01 00:00:00

abstract：:The ability of synthetic arginine-glycine-aspartic acid (RGD)-containing peptides to induce intracellular calcium transients similar to those observed at fertilization by spermatozoa in the bovine has been reported (Campbell et al., 2000: Biol Reprod 62:1702-1709; Sessions et al., 2006. Mol Reprod Dev). These results ...

journal_title：Molecular reproduction and development

authors： White KL,Passipieri M,Bunch TD,Campbell KD,Pate B

更新日期：2007-01-01 00:00:00

abstract：:Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...

journal_title：Molecular reproduction and development

authors： Wang MK,Liu JL,Li GP,Lian L,Chen DY

更新日期：2001-04-01 00:00:00

abstract：:Remodeling and relaxation of the mouse pubic symphysis (PS) are central events in parturition. The mouse PS remodels in a hormone-controlled process that involves the modification of the fibrocartilage into an interpubic ligament (IpL), followed by its relaxation prior to parturition. It is recognized that nitric oxid...

journal_title：Molecular reproduction and development

authors： Moro CF,Consonni SR,Rosa RG,Nascimento MA,Joazeiro PP

更新日期：2012-04-01 00:00:00

abstract：:We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT ge...

journal_title：Molecular reproduction and development

authors： Matsumoto K,Kakidani H,Takahashi A,Nakagata N,Anzai M,Matsuzaki Y,Takahashi Y,Miyata K,Utsumi K,Iritani A

更新日期：1993-09-01 00:00:00

abstract：:The aim of this study was to evaluate the role of adiponectin in the delayed embryonic development of Cynopterus sphinx. Adiponectin receptor (ADIPOR1) abundance was first observed to be lower during the delayed versus non-delayed periods of utero-embryonic unit development. The effects of adiponectin treatment on emb...

journal_title：Molecular reproduction and development

authors： Anuradha,Krishna A

更新日期：2014-12-01 00:00:00

abstract：:In the rat, in response to blastocyst implantation, stromal cells of the endometrium proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression. This phenomenon appears to be due to an active process involving apoptosis. As there is ...

journal_title：Molecular reproduction and development

authors： Almeida A,Correia-da-Silva G,Cepa M,Bell SC,Teixeira NA

更新日期：2007-03-01 00:00:00

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...

journal_title：Molecular reproduction and development

authors： Lee SG,Park CH,Choi DH,Kim HS,Ka HH,Lee CK

更新日期：2007-11-01 00:00:00

abstract：:Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...

journal_title：Molecular reproduction and development

authors： Ergenc AF,Li MW,Toner M,Biggers JD,Lloyd KC,Olgac N

更新日期：2008-12-01 00:00:00

abstract：:We describe the cloning and characterization of a partial cDNA, x121, that represents an RNA, which is localized in the animal hemisphere of Xenopus oocytes. This RNA is also detected in an animal to vegetal gradient during early cleavage stages. The x121 RNA titer decreases from fertilization through the gastrula sta...

journal_title：Molecular reproduction and development

authors： Kloc M,Reddy BA,Miller M,Eastman E,Etkin LD

更新日期：1991-04-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

journal_title：Molecular reproduction and development

authors： Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

更新日期：2008-06-01 00:00:00

abstract：:The murine intracisternal A particle (IAP) proviral elements are expressed at low levels in undifferentiated F9 embryonal carcinoma cells but are highly expressed when F9 cells are induced to differentiate into parietal endoderm-like cells. IAP elements are also expressed in parietal endoderm-like PYS-2 cells. We prev...

journal_title：Molecular reproduction and development

authors： Kamat JP,Basu A,Satyamoorthy K,Xu MQ,Showe L,Howe CC

更新日期：1995-05-01 00:00:00

abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

journal_title：Molecular reproduction and development

authors： Martin RH,Chernos JE,Rademaker AW

更新日期：1991-10-01 00:00:00

abstract：:Implantation is a complex process that requires the interaction of the blastocyst, and subsequently, that of the developing embryos with the endometrium. Several growth factors and cytokines are involved in implantation, but the details of their actions as related to the regulation of blastocyst implantation remain un...

journal_title：Molecular reproduction and development

authors： Taniguchi F,Harada T,Yoshida S,Iwabe T,Onohara Y,Tanikawa M,Terakawa N

更新日期：1998-05-01 00:00:00

abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...

journal_title：Molecular reproduction and development

authors： Harris MS,Rodger JC

更新日期：1998-08-01 00:00:00

abstract：:For mammalian organism, fertilization begins with species-specific recognition between sperm and egg, a process depending upon egg zona pellucida glycoproteins and putative sperm interacting protein(s). In mouse, zona pellucida glycoprotein ZP3 is believed to be the primary receptor for sperm and inducer of sperm acro...

journal_title：Molecular reproduction and development

authors： Li D,Cao S,Xu C

更新日期：2007-10-01 00:00:00

abstract：:The IGFs (IGF-I and IGF-II) are essential for normal mammalian growth and development. Their actions are mediated primarily by their interactions with the type I IGF receptor (IGF-I receptor), a transmembrane tyrosine kinase. The ligands and the IGF-I receptor are structurally related to insulin and to the insulin rec...

journal_title：Molecular reproduction and development

authors： LeRoith D,Kavsan VM,Koval AP,Roberts CT Jr

更新日期：1993-08-01 00:00:00

abstract：:The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...

journal_title：Molecular reproduction and development

authors： Allan ER,Habibi HR

更新日期：2012-09-01 00:00:00

abstract：:In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...

journal_title：Molecular reproduction and development

authors： Phillips KP,Baltz JM

更新日期：1996-09-01 00:00:00

abstract：:In mouse oocytes, meiotic induction by pharmacological activation of PRKA (adenosine monophosphate-activated protein kinase; formerly known as AMPK) or by hormones depends on stimulation of fatty acid oxidation (FAO). PRKA stimulates FAO by phosphorylating and inactivating acetyl CoA carboxylase (ACAC; formerly ACC), ...

journal_title：Molecular reproduction and development

authors： Valsangkar DS,Downs SM

更新日期：2015-09-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:Altering incubation temperature during embryogenesis has an impact on chicken embryo growth, but the underlying molecular mechanisms are not understood; the present study was performed to address these changes. Broiler eggs were incubated at low (36.8°C), control (37.8°C), and high (38.8°C) temperatures between Embryo...

journal_title：Molecular reproduction and development

authors： Krischek C,Janisch S,Naraballobh W,Brunner R,Wimmers K,Wicke M

更新日期：2016-01-01 00:00:00

abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...

journal_title：Molecular reproduction and development

authors： Zhang R,Zuo Y,Cao S

更新日期：2021-01-28 00:00:00

abstract：:Both ovarian and pituitary hormones are required for the pubertal development of the mouse mammary gland. Estradiol directs ductal elongation and branching, while progesterone leads to tertiary branching and alveolar development. The purpose of this investigation was to identify estrogen-responsive genes associated wi...

journal_title：Molecular reproduction and development

authors： Deroo BJ,Hewitt SC,Collins JB,Grissom SF,Hamilton KJ,Korach KS

更新日期：2009-08-01 00:00:00