Abstract:
:Both ovarian and pituitary hormones are required for the pubertal development of the mouse mammary gland. Estradiol directs ductal elongation and branching, while progesterone leads to tertiary branching and alveolar development. The purpose of this investigation was to identify estrogen-responsive genes associated with pubertal ductal growth in the mouse mammary gland in the absence of other ovarian hormones and at different stages of development. We hypothesized that the estrogen-induced genes and their associated functions at early stages of ductal elongation would be distinct from those induced after significant ductal elongation had occurred. Therefore, ovariectomized prepubertal mice were exposed to 17beta-estradiol from two to 28 days, and mammary gland global gene expression analyzed by microarray analysis at various times during this period. We found that: (a) gene expression changes in our estrogen-only model mimic those changes that occur in normal pubertal development in intact mice, (b) both distinct and overlapping gene profiles were observed at varying extents of ductal elongation, and (c) cell proliferation, the immune response, and metabolism/catabolism were the most common functional categories associated with mammary ductal growth. Particularly striking was the novel observation that genes active during carbohydrate metabolism were rapidly and robustly decreased in response to estradiol. Lastly, we identified mammary estradiol-responsive genes that are also co-expressed with estrogen receptor alpha in human breast cancer. In conclusion, our genomic data support the physiological observation that estradiol is one of the primary hormonal signals driving ductal elongation during pubertal mammary development.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Deroo BJ,Hewitt SC,Collins JB,Grissom SF,Hamilton KJ,Korach KSdoi
10.1002/mrd.21041subject
Has Abstractpub_date
2009-08-01 00:00:00pages
733-50issue
8eissn
1040-452Xissn
1098-2795journal_volume
76pub_type
杂志文章abstract::Glutamine (GLN) is a metabolic precursor for hexosamine synthesis and its inclusion in culture medium has been reported to improve cumulus expansion. Glutamine and cysteine share the same transport system. Excess external GLN may act as a competitive inhibitor for the uptake of cysteine and stimulate loss of cellular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199809)51:1<76::AID-MRD9>3
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abstract::In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.1080400206
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330302
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20540
更新日期:2006-12-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.22493
更新日期:2015-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20272
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.20435
更新日期:2006-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22801
更新日期:2017-05-01 00:00:00
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journal_title:Molecular reproduction and development
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doi:10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>
更新日期:1996-12-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199801)49:1<1::AID-MRD1>3.
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