Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.

abstract：:Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...

journal_title：Molecular reproduction and development

authors： Jalali BM,Kitewska A,Wasielak M,Bodek G,Bogacki M

更新日期：2014-03-01 00:00:00

abstract：:Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...

journal_title：Molecular reproduction and development

authors： Kim M,Geum D,Khang I,Park YM,Kang BM,Lee KA,Kim K

更新日期：2002-01-01 00:00:00

abstract：:A spontaneously established porcine granulosa cell line (PGC-2) was cloned through the continuous culturing of primary granulosa cells collected from equine chorionic gonadotropin (eCG)-treated prepubertal gilts. This established cell line has undergone approximately 100 passages and shows contact-inhibition of growth...

journal_title：Molecular reproduction and development

authors： Kwan I,Farookhi R,Huynh HT,Murphy BD,Turner JD,Downey BR

更新日期：1996-11-01 00:00:00

abstract：:Ejaculated boar spermatozoa exhibit two types of hyperactivation: full and non-full. Full-type hyperactivation is characterized by the asymmetrical bending of the entire middle piece-principal piece and a twisting/figure-eight-like trajectory, and can be induced by simple incubation with CaCl2 after preincubation with...

journal_title：Molecular reproduction and development

authors： Otsuka N,Harayama H

更新日期：2017-11-01 00:00:00

abstract：:To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...

journal_title：Molecular reproduction and development

authors： Zakhartchenko V,Wolf E,Palma GA,Brem G

更新日期：1995-09-01 00:00:00

abstract：:Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...

journal_title：Molecular reproduction and development

authors： Brewis IA,Morton IE,Moore HD,England GC

更新日期：2001-12-01 00:00:00

abstract：:Progesterone (P) has previously been shown to rapidly increase free intracellular calcium concentration ([Ca2+]i), and subsequently to initiate the acrosome reaction (AR) in capacitated human sperm. The present study used cytochemical analysis of the AR, and spectrofluorometric determination of sperm [Ca2+]i and intra...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1996-12-01 00:00:00

abstract：:The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...

journal_title：Molecular reproduction and development

authors： Leighton PA,van de Lavoir MC,Diamond JH,Xia C,Etches RJ

更新日期：2008-07-01 00:00:00

abstract：:Three staining procedures to detect sperm acrosome integrity were compared via electron microscopy. Stains were applied to epididymal, freshly ejaculated, in vivo capacitated, and sonicated sperm cells in addition to spermatozoa displaying sequentially removed plasma and outer and inner acrosomal membranes. Sequential...

journal_title：Molecular reproduction and development

authors： McBride CE,Fayrer-Hosken RA,Srivastava PN,Brackett BG

更新日期：1990-05-01 00:00:00

abstract：:Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not...

journal_title：Molecular reproduction and development

authors： Moody MA,Cardona C,Simpson AJ,Smith TT,Travis AJ,Ostermeier GC

更新日期：2017-05-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:The ter (teratoma, chromosome 18) mutation causes a deficiency of primordial germ cells (PGCs) in ter/ter embryos from the ter congenic mouse strain at 8.0 days post coitum (dpc). In order to analyse the function of the ter gene, here we examined effects of conditioned medium (CM) from 14.5 dpc testicular and ovarian ...

journal_title：Molecular reproduction and development

authors： Takabayashi S,Sasaoka Y,Yamashita M,Tokumoto T,Ishikawa K,Noguchi M

更新日期：2001-11-01 00:00:00

abstract：:In the cow, cryopreserved semen is inseminated into the uterus, and most of sperm are removed by backflow and phagocytes. Nevertheless, the mechanism responsible for sperm phagocytosis is unclear. Here, we used cultured bovine uterine epithelial cells (BUECs) to investigate the uterine response to sperm and the mechan...

journal_title：Molecular reproduction and development

authors： Elweza AE,Ezz MA,Acosta TJ,Talukder AK,Shimizu T,Hayakawa H,Shimada M,Imakawa K,Zaghloul AH,Miyamoto A

更新日期：2018-03-01 00:00:00

abstract：:In this study we have examined the effect of insulin like growth factor I (IGF-I) and its interaction with gonadotropins in the presence or absence of granulosa cell coculture on in vitro oocyte maturation (IVM) and their subsequent embryonic development in buffalo. We also have examined the role of IGF-I alone or in ...

journal_title：Molecular reproduction and development

authors： Pawshe CH,Rao KB,Totey SM

更新日期：1998-03-01 00:00:00

abstract：:A group of low Mr (16 kDa-23 kDa) glycoproteins on ejaculated boar spermatozoa have been shown to have high affinity for homologous zona pellucida glycoproteins (ZPGPs). These ZPGP binding proteins are derived from seminal plasma as shown by their absence from epididymal spermatozoa and their presence in seminal plasm...

journal_title：Molecular reproduction and development

authors： Parry RV,Barker PJ,Jones R

更新日期：1992-09-01 00:00:00

abstract：:The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...

journal_title：Molecular reproduction and development

authors： Bottalico B,Noskova V,Pilka R,Larsson I,Domanski H,Casslén B,Hansson SR

更新日期：2007-10-01 00:00:00

abstract：:The homeobox-containing gene family plays a pivotal role in regulating, patterning, and axial morphogenesis in the developing embryo. But there is still very little known about the expression and function of these genes in mammalian oocytes and preimplantation stage embryos. In this study we have used degenerate prime...

journal_title：Molecular reproduction and development

authors： Ponsuksili S,Wimmers K,Adjaye J,Schellander K

更新日期：2001-11-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00

abstract：:Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...

journal_title：Molecular reproduction and development

authors： Choo KB,Chen HH,Cheng WT,Chang HS,Wang M

更新日期：2001-07-01 00:00:00

abstract：:Mouse half-morulae were cryopreserved less than or equal to 1, 3, 6, and 12 hr after bisection by the vitrification method using 25% glycerol and 25% 1,2-propanediol as cryoprotectant. The developmental rates of the frozen-thawed half-embryos to blastocysts in vitro were 77.8% (63/81), 82.0% (41/50), 92.1% (117/127), ...

journal_title：Molecular reproduction and development

authors： Nagashima H,Kobayashi K,Yamakawa H,Ogawa S

更新日期：1991-11-01 00:00:00

abstract：:Inhibin α (Inha) gene expression is regulated, in rat granulosa cells, via a cyclic 3',5'-adenosine monophosphate (AMP)-response element (CRE) found in a region of the promoter that is homologous to the human INHA promoter. We previously found that during in vitro cytotrophoblast differentiation, human INHA gene expre...

journal_title：Molecular reproduction and development

authors： Depoix CL,Debiève F,Hubinont C

更新日期：2014-11-01 00:00:00

abstract：:The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...

journal_title：Molecular reproduction and development

authors： Fulcher KD,Welch JE,Klapper DG,O'Brien DA,Eddy EM

更新日期：1995-12-01 00:00:00

abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

journal_title：Molecular reproduction and development

authors： Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

更新日期：2014-12-01 00:00:00

abstract：:The objective of this research was to evaluate if DNA hypomethylation in cells used as karyoplasts would improve development of bovine nuclear transplantation (NT) embryos. DNA from serum-fed (SF), serum-starved (SS), and 1, or 5 microM 5-azacytidine (5-aza-CR) treated cells was digested with a methylation sensitive e...

journal_title：Molecular reproduction and development

authors： Jones KL,Hill J,Shin TY,Lui L,Westhusin M

更新日期：2001-10-01 00:00:00

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:Following Northern analysis, GGT mRNA was found predominantly within the caput epididymides and kidney. The size of mRNAs for kidney, caput, corpus, and ductus deferens were 2.2, 2.3, 2.2, and 2.3 kb, respectively, whereas cauda showed a doublet of 2.2 and 2.3 kb. GGT transpeptidation and hydrolytic activity within ep...

journal_title：Molecular reproduction and development

authors： Hinton BT,Palladino MA,Mattmueller DR,Bard D,Good K

更新日期：1991-01-01 00:00:00

abstract：:An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...

journal_title：Molecular reproduction and development

authors： Moore A,Penfold LM,Johnson JL,Latchman DS,Moore HD

更新日期：1993-03-01 00:00:00

abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...

journal_title：Molecular reproduction and development

authors： Benson S,Rawson R,Killian C,Wilt F

更新日期：1991-07-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...

journal_title：Molecular reproduction and development

authors： Bajpai R,Lesperance J,Kim M,Terskikh AV

更新日期：2008-05-01 00:00:00

abstract：:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

journal_title：Molecular reproduction and development

authors： Mattmueller DR,Hinton BT

更新日期：1992-05-01 00:00:00