Novel growth factor supporting survival of murine primordial germ cells: evidence from conditioned medium of ter fetal gonadal somatic cells.

abstract：:This study was performed to investigate the effects of edaravone on hypoxia-induced trophoblast cell proliferation, apoptosis, and invasion. The trophoblast cell line HTR-8 (H8) was treated with cobalt chloride (CoCl2 ) with or without a 1-hr edaravone pretreatment, followed by assessment of intracellular reactive oxy...

journal_title：Molecular reproduction and development

authors： Zheng Y,Zhao Y,Luo Q,Liu X,Liu X,Hu Y,Zou L

更新日期：2016-07-01 00:00:00

abstract：:Amino acid analyses of nuclear basic proteins of an anuran amphibian, Rana catesbeiana, revealed that they are comprised of a full set of core histones and three types of lysine-rich, sperm-specific proteins. On the basis of their amino-acid compositions and partial amino-acid sequences of their trypsin-resistant core...

journal_title：Molecular reproduction and development

authors： Itoh T,Ausio J,Katagiri C

更新日期：1997-06-01 00:00:00

abstract：:We have isolated and characterized a cDNA clone corresponding to a new member of bep (butanol, extracted, proteins) Paracentrotus lividus multigene family coding for cell surface proteins. The cDNA, called bep3, encodes a 370 amino acid protein and shares the same structural organization in the coding region with othe...

journal_title：Molecular reproduction and development

authors： Montana G,Romancino DP,di Carlo MD

更新日期：1996-05-01 00:00:00

abstract：:The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...

journal_title：Molecular reproduction and development

authors： Jackowski S,Xu XX,Rock CO

更新日期：1997-01-01 00:00:00

abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...

journal_title：Molecular reproduction and development

authors： Roy A,Yan W,Burns KH,Matzuk MM

更新日期：2004-03-01 00:00:00

abstract：:Recently, mice and embryonic stem (ES) cells with allelic polymorphisms have been used extensively in the field of genetics and developmental biology. In this study, we examined whether intersubspecific hybrid mice and ES cells with these genotypes can be efficiently produced by intracytoplasmic sperm injection (ICSI)...

journal_title：Molecular reproduction and development

authors： Shinmen A,Honda A,Ohkawa M,Hirose M,Ogonuki N,Yuzuriha M,Miki H,Mochida K,Inoue K,Abe K,Ito M,Ogura A

更新日期：2007-09-01 00:00:00

abstract：:Temporal pattern of expression of Cu/Zn and Mn superoxide dismutases (SODs) was investigated in bovine oocytes and embryos produced in vitro in two different culture conditions and in vivo after superovulation. SODs were examined at a transcriptional level in single oocytes and embryos by reverse transcriptase-polymer...

journal_title：Molecular reproduction and development

authors： Lequarré AS,Feugang JM,Malhomme O,Donnay I,Massip A,Dessy F,Van Langendonckt A

更新日期：2001-01-01 00:00:00

abstract：:In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...

journal_title：Molecular reproduction and development

authors： Cabot B,Tseng YC,Crodian JS,Cabot R

更新日期：2017-12-01 00:00:00

abstract：:Progesterone (P) has previously been shown to rapidly increase free intracellular calcium concentration ([Ca2+]i), and subsequently to initiate the acrosome reaction (AR) in capacitated human sperm. The present study used cytochemical analysis of the AR, and spectrofluorometric determination of sperm [Ca2+]i and intra...

journal_title：Molecular reproduction and development

authors： Garcia MA,Meizel S

更新日期：1996-12-01 00:00:00

abstract：:The goals of this work were to create germ-cell-stage-specific cDNA libraries from mouse spermatogenic cells and to employ a novel two-step genetic screen to identify gene sequences present during the critical meiotic stage of spermatogenesis. Highly enriched germ-cell fractions were prepared from adult and juvenile m...

journal_title：Molecular reproduction and development

authors： Caldwell KA,Wiltshire T,Handel MA

更新日期：1996-04-01 00:00:00

abstract：:In Ciona intestinalis, sperm penetration through the egg vitelline coat is an essential event of fertilization. We investigated whether trypsin- and chymotrypsin-like enzymes are involved in this event. Inhibitors and peptide substrates for chymotrypsin-like enzymes blocked the overall process of fertilization in a co...

journal_title：Molecular reproduction and development

authors： Pinto MR,Hoshi M,Marino R,Amoroso A,De Santis R

更新日期：1990-08-01 00:00:00

abstract：:To investigate the decrease of transcription and to determine the localization of RNA molecules during spermiogenesis in the fern Scolopendrium, we have used the enzyme-gold electron microscope method. During cell differentiation, the labeling decreases over the cytoplasm and increases over the nucleus. In the latter,...

journal_title：Molecular reproduction and development

authors： Rejon E,Bajon C,Blaize A,Robert D

更新日期：1988-01-01 00:00:00

abstract：:Zona pellucida (ZP) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. The efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their ZP glycoproteins. A bonnet monkey (Macaca radiata) ovarian cDNA lambda gt11 library wa...

journal_title：Molecular reproduction and development

authors： Jethanandani P,Santhanam R,Gupta SK

更新日期：1998-06-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...

journal_title：Molecular reproduction and development

authors： Bianco B,Peluso C,Gava MM,Ghirelli-Filho M,Lipay MV,Lipay MA,Christofolini DM,Barbosa CP

更新日期：2011-09-01 00:00:00

abstract：:In the present study, we investigated the expression of cyclin A2 in mouse two-cell embryos to elucidate the role of cyclin A2 at the G2/M transition. Two forms of cyclin A2 on SDS-PAGE (an upper and a lower band) were detected in two-cell embryos synchronized at the M phase by nocodazole. To investigate the nature of...

journal_title：Molecular reproduction and development

authors： Ohashi A,Imai H,Minami N

更新日期：2003-12-01 00:00:00

abstract：:The presence of two ras-related proteins (22 and 23 kDa) was demonstrated in Xenopus embryonic extracts by selective immunoprecipitation using anti-ras monoclonal antibodies 142-24E05 and Y13-259. We further describe the cytological effects of the microinjection of anti-ras monoclonal antibody Y13-259 into early cleav...

journal_title：Molecular reproduction and development

authors： Miron MJ,Lanoix J,Paiement J

更新日期：1990-04-01 00:00:00

abstract：:The aim of the present series of experiments was to investigate the effect of the size of follicle from which the oocytes originate on their subsequent in vitro developmental ability. Ovarian follicles were isolated and grouped according to size (2-6 mm, > 6 mm). Primary oocytes were carefully liberated and grouped ac...

journal_title：Molecular reproduction and development

authors： Lonergan P,Monaghan P,Rizos D,Boland MP,Gordon I

更新日期：1994-01-01 00:00:00

abstract：:Germinal vesicle (GV) breakdown in mammalian oocytes is regulated by the activation of maturation promoting factor (MPF). We investigated a specific cdc2 kinase inhibitor, roscovitine, to maintain pig oocytes in the GV stage. Cumulus-oocyte complexes (COCs) were aspirated from slaughterhouse ovaries and cultured for 4...

journal_title：Molecular reproduction and development

authors： Ju JC,Tsay C,Ruan CW

更新日期：2003-04-01 00:00:00

abstract：:Successful fertilization in mammals depends on the sperm's ability to initiate intracellular Ca2+ oscillations in the egg, a phenomenon that is elicited by Sperm-oocyte activating factors (SOAFs), whose quantitative and/or qualitative defect might result in fertilization failure. One such proposed factor is Post-acros...

journal_title：Molecular reproduction and development

authors： Freour T,Barragan M,Torra-Massana M,Ferrer-Vaquer A,Vassena R

更新日期：2018-02-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:A number of cytokines and growth factors are known to modulate proliferation and differentiation of human endometrium. In this study, the expression of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and VEGF receptors, fms-like tyrosine kinase (Flt1) and kinase insert domain-containi...

journal_title：Molecular reproduction and development

authors： Wei P,Chen XL,Song XX,Han CS,Liu YX

更新日期：2004-08-01 00:00:00

abstract：:We have cloned and sequenced cDNAs encoding autoantigen 1 (AA1), a testis-specific protein and the major autoantigen of the guinea pig sperm acrosome. The cDNA predicts a precursor protein of 244 amino acids including a 21 amino acid hydrophobic, secretory signal sequence. The mature polypeptide is predicted to have a...

journal_title：Molecular reproduction and development

authors： Foster JA,Gerton GL

更新日期：1996-06-01 00:00:00

abstract：:Unlike in higher vertebrates, in fish it is not known whether the nerve supply of the testis can influence testicular functions or not. In addition to neurotransmitters, nerve terminals may release ATP and adenosine in the extracellular medium. On the assumption that these molecules might be released by fibers innerva...

journal_title：Molecular reproduction and development

authors： Loir M

更新日期：1999-08-01 00:00:00

abstract：:Mitochondria play an important role in the integration and transmission of cell death signals mediated by the Bcl-2 family proteins. Experiments were conducted to determine whether the anti-apoptotic peptides BH4 domain of Bcl-xL (TAT-BH4) and Bax inhibitor peptide (BIP) suppresses heat stress (HS) injury in oocytes b...

journal_title：Molecular reproduction and development

authors： Soto P,Smith LC

更新日期：2009-07-01 00:00:00

abstract：:The role of extracellular matrix (ECM) in the differentiation of tissue types was examined in embryos of Strongylocentrotus purpuratus. We have examined the expression of various tissue-specific molecular markers after disrupting the ECM by culturing embryos in the presence of beta-aminoproprionitrile fumarate (BAPN),...

journal_title：Molecular reproduction and development

authors： Benson S,Rawson R,Killian C,Wilt F

更新日期：1991-07-01 00:00:00

abstract：:Cattle consuming endophyte-infected tall fescue grass have an associated reduction in circulating progesterone and reduced reproductive rates. In this study, commercially available rat microarrays were used to analyze the gene expression in luteal tissues from heifers fed endophyte-free fescue, endophyte-infected fesc...

journal_title：Molecular reproduction and development

authors： Jones KL,King SS,Iqbal MJ

更新日期：2004-02-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:The influence of somatic cells on oocyte penetrability was studied during in vitro maturation. Four experiments were carried out. In the first, pig oocytes were fertilized in vitro immediately after collection (immature oocytes) or after being cultured for 44 hr with cumulus cells connected to the whole wall of the ex...

journal_title：Molecular reproduction and development

authors： Galeati G,Modina S,Lauria A,Mattioli M

更新日期：1991-05-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00