Abstract:
:Zona pellucida (ZP) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. The efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their ZP glycoproteins. A bonnet monkey (Macaca radiata) ovarian cDNA lambda gt11 library was screened for ZP2 (bZP2) using full-length human ZP2 cDNA as a probe. Two identical full-length clones with an open reading frame of 2235 nt encoding a polypeptide of 745 aa residues were isolated. The deduced aa sequence of bZP2 revealed high sequence identity (94.2%) with human ZP2. The bZP2 cDNA (115-1914 nt, 1.8 kb), excluding sequences coding for N-terminal signal sequence and C-terminal transmembranelike domain, was PCR amplified and Sac1-Sal1 restricted fragment cloned in frame downstream of the T5 promoter under the lac operator control in a pQE-30 vector. Recombinant bZP2 (r-bZP2) was expressed as a polyhistidine fusion protein in Escherichia coli strain M15 [pREP4]. Immunoblot with rabbit polyclonal antibodies against bZP2 synthetic peptide (corresponding to aa residues 429-444; K434 replaced by R and 1436 by V) revealed a major band of 68 kDa. Immunization of male rabbits with the r-bZP2 protein purified on Ni-NTA resin under denaturing conditions generated antibodies reactive with r-bZP2 in ELISA as well as with native protein as revealed by positive fluorescence of ZP of bonnet monkey ovary. The availability of r-bZP2 and its aa sequence will help in the development and evaluation of a contraceptive vaccine based on ZP2.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Jethanandani P,Santhanam R,Gupta SKdoi
10.1002/(SICI)1098-2795(199806)50:2<229::AID-MRD14subject
Has Abstractpub_date
1998-06-01 00:00:00pages
229-39issue
2eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199806)50:2<229::AID-MRD14journal_volume
50pub_type
杂志文章abstract::Pigs have a unique, non-invasive epitheliochorial placenta where maternal and fetal layers lay in apposition. Indentation of fetal capillaries into the trophoblasts and maternal capillaries into the uterine epithelium reduce the distance between the fetal and maternal blood, ensuring nutrient transfer for proper conce...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22847
更新日期:2017-09-01 00:00:00
abstract::Ran, a Ras-related GTPase, is required for transporting proteins in and out of the nucleus during interphase and for regulating the assembly of microtubules. cDNA cloning shows that rat testis, like mouse testis, expresses both somatic and testis-specific forms of Ran-GTPase. The presence of a homologous testis-specif...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10164
更新日期:2002-09-01 00:00:00
abstract::This study was designed to examine the recovery of mitochondrial function and endogenous antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro maturation, bovine meiosis-II oocytes were vitrified, and then surviving oocytes were cultured an additional 8 hr. ATP content, ATP synt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21389
更新日期:2011-12-01 00:00:00
abstract::The distribution of microtubules was studied during fertilization of the rabbit oocyte by immunofluorescence microscopy after staining with an anti-alpha-tubulin antibody. In ovulated oocytes, microtubules were found exclusively in the meiotic spindle. At fertilization, the paternal centrosome generated sperm astral m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320313
更新日期:1992-07-01 00:00:00
abstract::Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) ha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20038
更新日期:2004-04-01 00:00:00
abstract::Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200008)56:4<502::AID-MRD8>3.0.CO
更新日期:2000-08-01 00:00:00
abstract::The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...
journal_title:Molecular reproduction and development
pub_type: 传,历史文章,杂志文章
doi:10.1002/mrd.21087
更新日期:2009-10-01 00:00:00
abstract::Production of transgenic cattle by microinjection of DNA has been difficult and costly. To explore an alternative method, one- to four-cell bovine embryos were exposed to a replication-defective retrovirus by microinjection of retrovirus producer cells into the perivitelline space. Embryos were cultured in vitro for 3...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400316
更新日期:1995-03-01 00:00:00
abstract::The localization of fibrillarin and nucleolin in the nuclei of mouse two-cell, four-cell, and eight-cell embryos has been studied using immunofluorescent staining with specific antibodies. In all of these cleavage stages, both antigens were associated exclusively with the peripheral region of the nucleolus precursor b...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400306
更新日期:1995-03-01 00:00:00
abstract::Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199612)45:4<431::AID-MRD4>
更新日期:1996-12-01 00:00:00
abstract::The expression of stem cell factor (SCF) mRNA and protein was examined in the postnatal rat testis. Northern blot analysis of total RNA from 1-4 days postpartum (dpp) testes showed none or barely detectable levels of the approximately 6.5 kb SCF transcript. At 5 dpp, there was a striking elevation in this mRNA, a timi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199705)47:1<19::AID-MRD3>3
更新日期:1997-05-01 00:00:00
abstract::The aim of this study was to determine the influence of hamster oviductal glycoprotein (oviductin) on in vitro gamete interaction. Oviductin was purified from the oviducts using lithium 3,5-diiodosalicylate, followed by phenol extraction. Immunocytochemistry using indirect fluorescence staining revealed that oviductin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080390309
更新日期:1994-11-01 00:00:00
abstract::Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23139
更新日期:2019-06-01 00:00:00
abstract::Activation of the mammalian egg results in cortical reaction (CR), which is correlated with an increase in intracellular Ca2+ concentration and PKC activation. The CR is a gradual rather then an "all or none" response, and can be regulated by different concentrations of parthenogenetic activators. To evaluate the biol...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199811)51:3<295::AID-MRD9>
更新日期:1998-11-01 00:00:00
abstract::Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22158
更新日期:2013-03-01 00:00:00
abstract::The spermatogonia of fish can be classified as being either undifferentiated type A spermatogonia or differentiated type B spermatogonia. Although type A spermatogonia, which contain spermatogonial stem cells, have been demonstrated to be a suitable material for germ cell transplantation, no molecular markers for dist...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20947
更新日期:2009-03-01 00:00:00
abstract::Basigin, a transmembrane glycoprotein belonging to the immunoglobulin superfamily, has been shown to be essential for fertilization and implantation. The aim of this study was to determine the expression and hormonal regulation of basigin gene in mouse uterus during the peri-implantation period. Basigin immunostaining...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10128
更新日期:2002-09-01 00:00:00
abstract::Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21224
更新日期:2010-09-01 00:00:00
abstract::The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20207
更新日期:2005-03-01 00:00:00
abstract::The objective of this study was to evaluate the effect of progesterone (P4), estradiol (E2), and cortisol (CO) at intraoviductal concentrations on bovine embryo development and quality in vitro. After fertilization of in vitro matured oocytes, zygotes were cultured for 8 days in synthetic oviductal fluid, supplemented...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23144
更新日期:2019-06-01 00:00:00
abstract::Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated t...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199910)54:2<112::AID-MRD2>
更新日期:1999-10-01 00:00:00
abstract::Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410405
更新日期:1995-08-01 00:00:00
abstract::Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of M...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22495
更新日期:2015-06-01 00:00:00
abstract::A porcine interleukin-6 (pIL-6) cDNA has been cloned from pig spleen cDNA library to provide information that would allow us to study IL-6 mRNA expression during pregnancy of several domestic Artiodactyla. The cDNA is 1058 bp long and with a single open reading frame that encodes a 212 amino acid polypeptide with 28-r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320404
更新日期:1992-08-01 00:00:00
abstract::The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300213
更新日期:1991-10-01 00:00:00
abstract::P450aromatase (CYP19) is an essential part of the enzyme complex catalyzing the conversion of androgens into estrogens, which is a key reaction in the sex differentiation in vertebrates. Two full-length cDNAs encoding the genetic distinct CYP19A and CYP19B isozymes were isolated from Atlantic halibut (Hippoglossus hip...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20373
更新日期:2005-12-01 00:00:00
abstract::Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10015
更新日期:2002-03-01 00:00:00
abstract::Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080270311
更新日期:1990-11-01 00:00:00
abstract::In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP-dependent protein kinase in sperm motion. In the flagellum, a human-specific isoform of the RII alpha subunit was located on the a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080390410
更新日期:1994-12-01 00:00:00
abstract::Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22309
更新日期:2014-05-01 00:00:00