Abstract:
:Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capacitation. The majority of the labeled seminal plasma protein was also low Mr. After capacitation, two new proteins (64,000 Mr and 78,000 Mr) were labeled. Sperm did not exhibit these exposed membrane proteins when incubated under noncapacitating conditions. Appearance of these proteins was not correlated to the percentage of acrosome-reacted sperm. Although the 64,000 Mr protein was not consistently observed, the relative labeling of the 78,000 Mr protein was highly correlated with the ability of sperm to fuse with zona-free hamster ova. The 78,000 Mr protein may be a sperm protein involved in fusion with the egg plasma membrane.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Berger Tdoi
10.1002/mrd.1080270311subject
Has Abstractpub_date
1990-11-01 00:00:00pages
249-53issue
3eissn
1040-452Xissn
1098-2795journal_volume
27pub_type
杂志文章abstract::We aimed in this study to explore if sphingosine-1-phosphate (S1P) reduces apoptosis of primordial follicles during cryopreservation of human ovarian cortical samples. Ovarian cortical tissue fragments obtained from young patients who underwent laparoscopic excision of benign ovarian cysts were used for the experiment...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23043
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更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1992-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1080310206
更新日期:1992-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-06-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-2795(199810)51:2<218::AID-MRD12
更新日期:1998-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080250409
更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199603)43:3<366::AID-MRD11
更新日期:1996-03-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:Molecular reproduction and development
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更新日期:1997-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080360115
更新日期:1993-09-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1998-09-01 00:00:00
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更新日期:2002-05-01 00:00:00
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更新日期:2009-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.1080280109
更新日期:1991-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20131
更新日期:2004-11-01 00:00:00