Abstract:
:Embryonal carcinoma (EC) cells are used widely as a model system for studying the expression of developmentally regulated genes, in particular genes that are regulated at the transcriptional level when EC cells differentiate. This review focuses on the molecular mechanisms that govern the transcription of the fibroblast growth factor-4 (FGF-4) gene, which appears to be the first FGF expressed during mammalian development. Interest in this gene has increased considerably with the finding that FGF-4 is essential for mammalian embryogenesis. The FGF-4 gene has also generated considerable interest because it is inhibited at the transcriptional level when EC cells undergo differentiation and because this gene is regulated by a powerful distal enhancer located 3 kb downstream of the transcription start site in the last exon of the gene. Hence, study of the FGF-4 gene is likely to shed light on the molecular mechanisms by which distal enhancers regulate gene expression. In addition to being regulated by the downstream enhancer, the expression of this gene is influenced by a regulatory region located just upstream of the transcription start site, which contains two Sp1 motifs and a CCAAT box motif. Examination of the downstream enhancer has identified three functional cis-regulatory elements: a high mobility group (HMG) protein binding motif, an octamer binding motif, and an Sp1 motif, which are likely to bind Sox-2, Oct-3, and Sp1/Sp3, respectively, in vivo. Interestingly, Sox-2 and Oct-3 expression, like FGF-4 expression, decreases when EC cells differentiate, which suggests that the loss of these transcription factors is responsible, at least in part, for the transcriptional turn-off of the FGF-4 gene. In view of these and other findings, we present a model for the differential expression of the FGF-4 gene that includes not only the contributions of specific transcription factors, but also the contribution of chromatin structure before and after differentiation.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Lamb KA,Rizzino Adoi
10.1002/(SICI)1098-2795(199810)51:2<218::AID-MRD12subject
Has Abstractpub_date
1998-10-01 00:00:00pages
218-24issue
2eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199810)51:2<218::AID-MRD12journal_volume
51pub_type
杂志文章,评审abstract::Estrogen plays an important role in the human reproductive system and its action is mediated mainly by two specific receptors: α (ERα) and β (ERβ). There are polymorphic variants in both ER genes, and studies showed their association with reproductive outcomes. We aimed to determine the distribution of ERα and ERβ gen...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21365
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abstract::The effect of reactive oxygen species production on the motility parameters, DNA integrity, acid phosphatase activity, and protein tyrosine phosphorylation in spermatozoa of the common carp (Cyprinus carpio) was investigated. Spermatozoa were exposed to different concentrations of xanthine and xanthine oxidase (X-XO) ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22442
更新日期:2015-01-01 00:00:00
abstract::Teleost fish exhibit remarkably diverse and plastic patterns of sexual development. One of the most fascinating modes of plasticity is functional sex change, which is widespread in marine fish including species of commercial importance; however, the regulatory mechanisms remain elusive. In this review, we explore such...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
abstract::Implantation is a complex process that requires the interaction of the blastocyst, and subsequently, that of the developing embryos with the endometrium. Several growth factors and cytokines are involved in implantation, but the details of their actions as related to the regulation of blastocyst implantation remain un...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199805)50:1<54::AID-MRD7>3
更新日期:1998-05-01 00:00:00
abstract::The aim of this study was to determine the influence of hamster oviductal glycoprotein (oviductin) on in vitro gamete interaction. Oviductin was purified from the oviducts using lithium 3,5-diiodosalicylate, followed by phenol extraction. Immunocytochemistry using indirect fluorescence staining revealed that oviductin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080390309
更新日期:1994-11-01 00:00:00
abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20762
更新日期:2008-01-01 00:00:00
abstract::cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420204
更新日期:1995-10-01 00:00:00
abstract::Sex-related growth rate differences in preimplantation mouse embryos were investigated. In experiment I, Day 3 embryos were recovered from reproductive tracts, classified according to developmental stage, and cultured for 24 hr in CZB medium containing glucose. Each embryo was then reclassified and stained for measure...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400108
更新日期:1995-01-01 00:00:00
abstract::Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22227
更新日期:2013-11-01 00:00:00
abstract::Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22801
更新日期:2017-05-01 00:00:00
abstract::Acrosin (ACR), a serine proteinase located in the acrosome of the sperm, has been presumed to be involved in the recognition and binding of the sperm to the zona pellucida of the ovum and the sperm penetration through the zona pellucida. To examine the function of acrosin in vivo, we have generated mice carrying a mut...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199703)46:3<370::AID-MRD16
更新日期:1997-03-01 00:00:00
abstract::Collagen is denatured in the gradual cervical ripening process during late pregnancy, already before the onset of final cervical ripening at parturition. Matrix Metallo Proteinases (MMPs) might be responsible for this process. To investigate the presence and potential function of MMPs at the different stages of the ri...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20908
更新日期:2008-11-01 00:00:00
abstract::An organized microtubular cytoskeleton was discovered in the cytoplasm of Xenopus laevis oocytes. The microtubules were observed in 10- to 30-micron cryostat sections by indirect immunoperoxidase labeling using an antibody to tubulin. A gradual extraction of cells with a nonionic detergent was essential for good penet...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300410
更新日期:1991-12-01 00:00:00
abstract::These experiments were designed to test the effects of an electrofusion and an electroporation pulse on bovine sperm-hamster egg development. In experiment 1, single motile sperm were injected into the perivitelline space of each egg. A 4,500 V/cm, 30 microseconds fusion pulse (FP) was applied while sperm-egg membrane...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080270212
更新日期:1990-10-01 00:00:00
abstract::Previously, we showed that the gonadotropin-induced expansion of bovine cumulus oophorus occurs concomitantly with the rearrangement of microfilaments (MFs) inside cumulus cell cytoplasm (Sutovský et al., 1993: Biol Reprod 49:1277-1287; Sutovský et al., 1994: Reprod Nutr Dev 34:415-425) and that cumulus expansion in c...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410415
更新日期:1995-08-01 00:00:00
abstract::We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the ma...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20298
更新日期:2005-08-01 00:00:00
abstract::In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20678
更新日期:2007-08-01 00:00:00
abstract::Hoxa10, a homeodomain transcription factor, is dynamically expressed in adult uterine endometrium where it is necessary for embryo implantation. Endometrial Hoxa10 expression is driven by estrogen and progesterone. High levels of endometrial Hoxa10 expression coincide with high progesterone levels and development of e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20013
更新日期:2004-01-01 00:00:00
abstract::The distribution of microtubules was studied during fertilization of the rabbit oocyte by immunofluorescence microscopy after staining with an anti-alpha-tubulin antibody. In ovulated oocytes, microtubules were found exclusively in the meiotic spindle. At fertilization, the paternal centrosome generated sperm astral m...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080320313
更新日期:1992-07-01 00:00:00
abstract::The present study investigated the effects of triiodothyronine (T3) on pituitary gonadotropin (GTH) subunits, thyroid stimulating hormone (TSH) β subunit, and growth hormone (GH) mRNA levels, as well as gonadal steroid secretion during different stages of reproduction in goldfish. Goldfish pituitary cells cultured wit...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22066
更新日期:2012-09-01 00:00:00
abstract::The aim of this study was to examine the expression and regulation of peroxisome proliferator-activated receptor (PPAR) PPARdelta gene in mouse uterus during early pregnancy by in situ hybridization and immunohistochemistry. PPARdelta expression under pseudopregnancy, delayed implantation, hormonal treatment, and arti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10348
更新日期:2003-11-01 00:00:00
abstract::Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420303
更新日期:1995-11-01 00:00:00
abstract::The morphological analysis of the spermatozoa from fertile and infertile men was performed using light and electron microscopy to clarify the relationship between sperm morphology and fertility. Semen samples obtained from 22 partners of pregnant women were prepared according to the protocol standardized in an interna...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20189
更新日期:2005-01-01 00:00:00
abstract::The genome of germline committed cells is thought to be protected by mechanisms of transcriptional silencing, posing a barrier to transgenesis using cultured germline cells. We found that selection for transgene integration into the primordial germ cell genome required that the transgenes be flanked by the chicken bet...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20859
更新日期:2008-07-01 00:00:00
abstract::Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20819
更新日期:2008-05-01 00:00:00
abstract::Configuration of germinal vesicle (GV) chromatin has been studied and found correlated with the developmental competence of oocytes in several mammalian species. A common feature in the configuration of GV chromatin in the species studied so far is that the diffuse chromatin (the so called "NSN" pattern) condenses int...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20251
更新日期:2005-06-01 00:00:00
abstract::We have previously reported metabolic cooperation between Sertoli and peritubular myoid cells in terms of synthesis of one of the main testicular extracellular matrix (ECM) constituents, glycosaminoglycans (GAG). This study concerns Sertoli cell ECM-peritubular myoid cell interactions in terms of GAG synthesis. We hav...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350208
更新日期:1993-06-01 00:00:00
abstract::The present studies were performed to establish the effects of the size and number of artificial holes produced in the zona pellucida (ZP) on hatching and trophoblast outgrowth in vitro. Limited partial zona dissection (PZD) produced small, narrow incisions, and zona drilling with acidic Tyrode's (AT) across a larger ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080300110
更新日期:1991-09-01 00:00:00
abstract::We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20731
更新日期:2007-11-01 00:00:00
abstract::In this report we show that the first event of activation in the human oocyte, the Fertilization current (FC), is a slow transient outward current of 300 pA, which induces a gradual hyperpolarization of the plasma membrane from -20mV to -60mV, 60-120 min after insemination, followed by a repolarization to -20mV. Activ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080380212
更新日期:1994-06-01 00:00:00