Abstract:
:We have cloned and sequenced the bovine NALP9 cDNA, which contains a 2991 bp long open reading frame with 76% homology to its human counterpart. The putative 996 amino acids protein presents the domain organization typical of the Nacht, Leucine rich repeat and Pyrin domain containing (NALP) family that includes the maternal effect factor MATER. By reverse transcription coupled to polymerase chain reaction, we have analyzed expression of NALP9 in a panel of somatic and gonadic tissues and during in vitro preimplantation development. The transcript was detected exclusively in testis and ovary, and at a remarkably high level in the oocyte from antral follicles. NALP9 mRNA remained present after in vitro maturation and fertilization, and was detected in embryos, but transcription was not reactivated at the maternal to embryo transition. Thus NALP9 is characterized as a novel oocyte marker gene in cattle.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Dalbiès-Tran R,Papillier P,Pennetier S,Uzbekova S,Monget Pdoi
10.1002/mrd.20298keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
414-21issue
4eissn
1040-452Xissn
1098-2795journal_volume
71pub_type
杂志文章abstract::Infusion of seminal plasma in the uterus is known to elicit an instant inflammatory response in the porcine uterus, but whether or not it prepares a uterine immunological response to the presence of conceptuses is not well understood. Seminal plasma induced long-term modulatory effects and conceptus-induced immune cha...
journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
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更新日期:2017-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-04-01 00:00:00
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journal_title:Molecular reproduction and development
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pub_type: 杂志文章
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pub_type: 杂志文章
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