Abstract:
:cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein similar to the human prostatic secretory protein, PSP94, which had been originally identified in male genital organs. The expression was detected widely in the functional corpora lutea by in situ hybridization histochemistry, but the signal was most prominent in the luteal cells surrounding blood vessels. In contrast, despite the structural similarity in their products, the expression of this newly identified gene was not detected in porcine prostate, suggesting that these structurally related proteins play different roles in mammalian genital organs.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Tanaka T,Itahana K,Andoh N,Takeya T,Sato Edoi
10.1002/mrd.1080420204subject
Has Abstractpub_date
1995-10-01 00:00:00pages
149-56issue
2eissn
1040-452Xissn
1098-2795journal_volume
42pub_type
杂志文章abstract::Seminiferous tubules of the testis and epididymal tubules in adult rodents are enveloped by contractile myoid cells, which move sperm and fluids along the male reproductive tract. Myoid cells in the testis influence Sertoli cells by paracrine signaling, but their role in the epididymis is unknown. Electron microscopy ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22649
更新日期:2016-06-01 00:00:00
abstract::An in vitro superfusion method was used to test sex hormone release from different kinds of ovarian follicle (growing follicles, postovulatory follicles, and atretic follicles) in the lizard Podarcis sicula sicula. Sex hormone output changes with the stage of follicle evolution and sexual cycle. Previtellogenetic foll...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080350307
更新日期:1993-07-01 00:00:00
abstract::Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10293
更新日期:2003-05-01 00:00:00
abstract::Decades worth of research have consistently shown the adverse effects of elevated temperatures on reproductive parameters of livestock species. The objective of this study was to evaluate the developmental and apoptotic responses of porcine in vitro fertilized (IVF) and parthenogenetically activated (PA) embryos heat ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20620
更新日期:2007-05-01 00:00:00
abstract::Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20485
更新日期:2006-09-01 00:00:00
abstract::The 26S proteasome is a self-compartmentalizing protease responsible for the degradation of intracellular proteins. This giant intracellular protease is formed by several subunits arranged into two 19S polar caps-where protein recognition and ATP-dependent unfolding occur-flanking a 20S central barrel-shaped structure...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/1098-2795(200010)57:2<109::AID-MRD1>3.0.CO
更新日期:2000-10-01 00:00:00
abstract::One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20540
更新日期:2006-12-01 00:00:00
abstract::Production of embryos in vitro has enormous potential for research and commercial applications. Unfortunately, in vitro production of porcine embryos is extremely inefficient. Despite the characterization of distinct phenotypes, little is known about the molecular mechanisms and altered physiological processes of in v...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20844
更新日期:2008-06-01 00:00:00
abstract::Zona pellucida (ZP) glycoproteins have been proposed as candidate antigens for an immunocontraceptive vaccine. The efficacy of such a vaccine has to be evaluated in nonhuman primates, thus necessitating the characterization of their ZP glycoproteins. A bonnet monkey (Macaca radiata) ovarian cDNA lambda gt11 library wa...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199806)50:2<229::AID-MRD14
更新日期:1998-06-01 00:00:00
abstract::Previously, we cloned and sequenced a sperm specific antigen, designated as HSS (EMBL nomenclature human sperm associated antigen 9: hSPAG9) from human testis (Shankar et al.: Biochem Biophys Res Commun 243:561-565, 1998). The present study was conducted to isolate baboon proteomic homologue in order to find out wheth...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20164
更新日期:2004-10-01 00:00:00
abstract::Lactate produced by glycolysis in Sertoli cells (SCs) is the main energy substrate for developing germ cells and plays a vital role in spermatogenesis. MicroRNAs (miRNAs) function as posttranscriptional regulators of gene expression in biological processes. We have previously shown that hyperthermia (43°C, 30 min) pro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23265
更新日期:2019-11-01 00:00:00
abstract::To study possible role and regulation of apoptosis occurred in primate endometrium, the expression of apoptosis-related molecules, Fas, FasL, B cell lymphoma/leukaemia-2 (Bcl-2), and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunoh...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20215
更新日期:2005-04-01 00:00:00
abstract::cAMP response element binding protein (CREB)-binding protein (CBP) and p300 are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes. Current dogma would suggest that these transcriptional co-activators have similar mechanisms of transcription regulation. Studies of ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20440
更新日期:2006-07-01 00:00:00
abstract::Both ovarian and pituitary hormones are required for the pubertal development of the mouse mammary gland. Estradiol directs ductal elongation and branching, while progesterone leads to tertiary branching and alveolar development. The purpose of this investigation was to identify estrogen-responsive genes associated wi...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21041
更新日期:2009-08-01 00:00:00
abstract::Sperm must undergo capacitation to become fertilization competent. Here we validated that monosialotetrahexosylganglioside (GM1 ) localization patterns, which were assessed in the Cap-Score™ Sperm Function Test, reflect a capacitated state in human sperm. First, we defined patterns representing sperm that do or do not...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22801
更新日期:2017-05-01 00:00:00
abstract::In this study, a combination of RNAi and endoscopic transfer to the oviduct of synchronized heifers has been used to investigate the effect of suppression of Cx43 and E-cadherin on the development, mRNA and protein expression of bovine blastocysts cultured in vitro or in vivo. In vitro matured and fertilized bovine zy...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20678
更新日期:2007-08-01 00:00:00
abstract::Oocyte maturation is an important process required to achieve optimal oocyte quality, and later affects fertilization potential and subsequent embryo development. The maturation process includes synchronized nuclear and cytoplasmic remodeling, in which cytoskeletal and centrosome dynamics play an important role and si...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21380
更新日期:2011-10-01 00:00:00
abstract::Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20710
更新日期:2007-10-01 00:00:00
abstract::To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420107
更新日期:1995-09-01 00:00:00
abstract::The mitochondrial probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) not only identifies mitochondria exhibiting low membrane potentials by the emission of green fluorescence (range, 510-520 nm) but also differentiates these from mitochondria exhibiting relatively high membrane ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199906)53:2<222::AID-MRD11
更新日期:1999-06-01 00:00:00
abstract::We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20731
更新日期:2007-11-01 00:00:00
abstract::The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330404
更新日期:1992-12-01 00:00:00
abstract::In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199609)45:1<52::AID-MRD8>3
更新日期:1996-09-01 00:00:00
abstract::We previously showed that, during mouse oocyte maturation, specific maternal mRNAs (actins) are deadenylated, while others (hypoxanthine phosphoribosyltransferase:HPRT) are adenylated. As in other systems, these changes can be correlated with changes in translational activities. Maturation-specific polyadenylation in ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370208
更新日期:1994-02-01 00:00:00
abstract::Endometriosis is regarded as a hormone-dependent disease. Current therapeutic approaches to treating this common gynecological disorder mainly depend on surgical and hormonal interventions, but the high rate of disease recurrence as well as the side effects related to such therapies make it difficult for patients to r...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.22493
更新日期:2015-06-01 00:00:00
abstract::We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22790
更新日期:2017-05-01 00:00:00
abstract::Topoisomerase II (topo II) is an essential enzyme that alters DNA topology. This activity is important for a variety of chromosome functions including DNA replication, transcription, recombination, and chromosome condensation and segregation. Previously we localized topo II in mouse gametes and preimplantation embryos...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10015
更新日期:2002-03-01 00:00:00
abstract::Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330213
更新日期:1992-10-01 00:00:00
abstract::Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20919
更新日期:2008-12-01 00:00:00
abstract::Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20025
更新日期:2004-03-01 00:00:00