Centrosome dynamics during mammalian oocyte maturation with a focus on meiotic spindle formation.

abstract：:The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium-sized follicles (3-8 mm in diameter) were untreated (MCO) or treated with ...

journal_title：Molecular reproduction and development

authors： You J,Kim J,Lee H,Hyun SH,Hansen PJ,Lee E

更新日期：2012-01-01 00:00:00

abstract：:Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...

journal_title：Molecular reproduction and development

authors： Russell DF,Ibáñez E,Albertini DF,Overström EW

更新日期：2005-10-01 00:00:00

abstract：:So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...

journal_title：Molecular reproduction and development

authors： Ola SI,Ai JS,Liu JH,Wang Q,Wang ZB,Chen DY,Sun QY

更新日期：2008-01-01 00:00:00

abstract：:The IGFs (IGF-I and IGF-II) are essential for normal mammalian growth and development. Their actions are mediated primarily by their interactions with the type I IGF receptor (IGF-I receptor), a transmembrane tyrosine kinase. The ligands and the IGF-I receptor are structurally related to insulin and to the insulin rec...

journal_title：Molecular reproduction and development

authors： LeRoith D,Kavsan VM,Koval AP,Roberts CT Jr

更新日期：1993-08-01 00:00:00

abstract：:In the present study we analyse the nature and the functional significance of the spherical and fibrillo-granular structures appearing in the oocyte nucleus of the lizard Podarcis sicula, following the disappearance of the typical nucleolus. By LM and TEM approaches, we demonstrate that the fibrillo-granuli, containin...

journal_title：Molecular reproduction and development

authors： Tammaro S,Andreuccetti P,Filosa S,Indolfi P,Prisco M,Motta CM

更新日期：1998-12-01 00:00:00

abstract：:Inner cell masses (ICM) from in vitro produced day 8 or 9 bovine blastocysts were isolated by immunosurgery and cultured under different conditions in order to establish which of two feeder cell types and culture media were most efficient in supporting attachment and outgrowth of the bovine ICM cells. The efficiency o...

journal_title：Molecular reproduction and development

authors： Van Stekelenburg-Hamers AE,Van Achterberg TA,Rebel HG,Fléchon JE,Campbell KH,Weima SM,Mummery CL

更新日期：1995-04-01 00:00:00

abstract：:This study investigated the mechanisms for the regulation of intracellular pH in bovine oocytes and embryos. Na(+)/H(+) antiporter activity for the regulation of intracellular pH in the acid to neutral range was detected in both in vitro matured bovine oocytes and in vitro produced embryos. However, the activity of th...

journal_title：Molecular reproduction and development

authors： Lane M,Bavister BD

更新日期：1999-12-01 00:00:00

abstract：:Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnR...

journal_title：Molecular reproduction and development

authors： Kanka J,Fulka J Jr,Fulka J,Petr J

更新日期：1991-06-01 00:00:00

abstract：:cAMP-dependent protein kinase A (PKA) plays important regulatory roles during mouse spermatogenesis. PKA-mediated signaling has been shown to regulate gene expression, chromatin condensation, capacitation, and motility during sperm development and behavior, although how PKA is regulated in spatiotemporal manners durin...

journal_title：Molecular reproduction and development

authors： Xu K,Qi H

更新日期：2014-07-01 00:00:00

abstract：:Previous studies from our laboratory have identified MPS, a 100-kDa protein, as the major phosphoprotein substrate of caprine sperm ecto-cyclic AMP independent protein kinase. In this study the isolated (32)P-labelled MPS has been incorporated into mature caprine (Capra indicus) cauda-epididymal spermatozoa with the h...

journal_title：Molecular reproduction and development

authors： Maiti A,Mishra KP,Majumder GC

更新日期：2008-07-01 00:00:00

abstract：:We have studied the presence of four sperm glycosidases, alpha-mannosidase, alpha-L- fucosidase and two beta-hexosaminidase isoforms, in 11 species of the genus Drosophila spanning approximately an evolutionary 60 MY period, and in Scaptodrosophila lebanonensis, belonging to the ancestor genus Scaptodrosophila. These ...

journal_title：Molecular reproduction and development

authors： Intra J,Cenni F,Pavesi G,Pasini M,Perotti ME

更新日期：2009-01-01 00:00:00

abstract：:Exposing day 5 bovine morulae to reactive oxygen species induces a delayed degeneration of some blastocysts on day 8 post-insemination (pi) but without affecting the blastocyst rates. The aim of this study was to characterize the resisting and the degenerating population of blastocysts. The kinetics of degeneration of...

journal_title：Molecular reproduction and development

authors： Feugang JM,Donnay I,Mermillod P,Marchandise J,Lequarre AS

更新日期：2005-07-01 00:00:00

abstract：:Tektins are microtubule-associated cytoskeletal proteins that are expressed primarily in the male germ cell-lineage in centrioles and basal bodies and within ciliary and flagellar doublet microtubules. They are proposed to be important for axonemal architecture and microtubule stability in the sperm tail and in other ...

journal_title：Molecular reproduction and development

authors： Roy A,Yan W,Burns KH,Matzuk MM

更新日期：2004-03-01 00:00:00

abstract：:The cumulus cells that surround the mammalian oocyte become dispersed following the preovulatory surge of the pituitary gonadotropin, luteinizing hormone (LH). We have examined cumulus-oocyte complexes of PMSG-primed immature rats before and at 1, 2, 3, 4, 6, and 8 hr after injection of human chorionic gonadotropin (h...

journal_title：Molecular reproduction and development

authors： Phillips DM,Dekel N

更新日期：1991-03-01 00:00:00

abstract：:Many ascidians live in clumps and usually release sperm before the eggs. Consequently, eggs are often spawned into dense clouds of sperm. Because fertilization by more than a single sperm is lethal, ascidians have evolved at least two successive blocks to polyspermy: the rapid release of a glycosidase that inhibits sp...

journal_title：Molecular reproduction and development

authors： Lambert C,Goudeau H,Franchet C,Lambert G,Goudeau M

更新日期：1997-09-01 00:00:00

abstract：:We have developed a novel reverse transcription-polymerase chain reaction (RT-PCR)-based approach for systematically quantifying in a single experiment the abundances of many different mRNAs in preimplantation mouse embryos. With this approach, the entire mRNA population from a small number of embryos is amplified whi...

journal_title：Molecular reproduction and development

authors： Rambhatla L,Patel B,Dhanasekaran N,Latham KE

更新日期：1995-07-01 00:00:00

abstract：:Sperm from the decapod crustacean Rhynchocinetes typus undergo dramatic shape changes as they pass from the vas deferens to seawater and interact with the oocyte envelopes. Using FITC-phalloidin and antitubulin antibodies, we were able to localize microfilaments and a tubulin-like protein in R. typus spermatozoon. Mic...

journal_title：Molecular reproduction and development

authors： Perez C,Roco M,Castro A,Dupre E,Schatten G,Barros C

更新日期：1991-04-01 00:00:00

abstract：:A quantitative primer extension method was used to measure the mass of histone gene transcripts in mature oocytes of the amphibian Xenopus laevis. The procedure, using a large excess of gene-specific oligonucleotide primer and continuous incorporation of a radiolabeled deoxynucleoside triphosphate precursor, is more s...

journal_title：Molecular reproduction and development

authors： Brashears-Macatee S,Hinkley C,Perry M

更新日期：1990-01-01 00:00:00

abstract：:Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...

journal_title：Molecular reproduction and development

authors： Kaufman MH,Lee KK,Speirs S

更新日期：1990-04-01 00:00:00

abstract：:Oocyte meiosis is arrested at prophase I by factors secreted from surrounding somatic cells after oocytes acquire meiotic competence at an early antral stage, and meiosis resumes in preovulatory follicles as a result of the luteinizing hormone (LH) surge. Recently, signaling by C-type natriuretic peptide (CNP) through...

journal_title：Molecular reproduction and development

authors： Tsuji T,Kiyosu C,Akiyama K,Kunieda T

更新日期：2012-11-01 00:00:00

abstract：:In vitro studies have suggested that the Cables1 gene regulates epithelial cell proliferation, whereas other studies suggest a role in promoting neural differentiation. In efforts to clarify the functions of Cables1 in vivo, we conducted gain- and loss-of-function studies targeting its ortholog (cables1) in the zebraf...

journal_title：Molecular reproduction and development

authors： Groeneweg JW,White YA,Kokel D,Peterson RT,Zukerberg LR,Berin I,Rueda BR,Wood AW

更新日期：2011-01-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:Although the epididymal environment promotes the maturation and survival of spermatozoa, not all spermatozoa remain viable during passage through the epididymis. Does the epididymis has a protective mechanism(s) to segregate the viable sperm from defective spermatozoa? Previously, we identified 260/280 kDa oligomers (...

journal_title：Molecular reproduction and development

authors： Nagdas SK,Wallace S,Eaford D,Baker R,Carr K,Raychoudhuri SS

更新日期：2020-12-01 00:00:00

abstract：:Mammalian reproductive processes involve spermatogenesis, which occurs in the testis, and fertilization, which takes place in the female genital tract. Fertilization is a successive, multistep, and extremely complicated event that usually includes sperm survival in the uterus, sperm migration through the uterotubal ju...

journal_title：Molecular reproduction and development

authors： Xiong W,Wang Z,Shen C

更新日期：2019-08-01 00:00:00

abstract：:Glycoprotein hormone receptors contain large extracellular domains encoded by multiple exons that can be alternatively spliced. Using human ovarian surface epithelium, we cloned two new splice variants of the human follicle-stimulating hormone receptor (FSH-R) gene, hFSH-R2 and hFSH-R3. The hFSH-R2 splice variant diff...

journal_title：Molecular reproduction and development

authors： Zhou J,Huang Y,Li L,Zhu L,Zhang D,Zhang S,Chen Y

更新日期：2013-11-01 00:00:00

abstract：:In the sea urchin, some other marine invertebrates, and the frog, Xenopus, egg activation at fertilization is accompanied by an increase in intracellular pH (pHi). We measured pHi in germinal vesicle (GV)-intact mouse oocytes, ovulated eggs, and in vivo fertilized zygotes using the pH indicator dye, SNARF-1. The mean ...

journal_title：Molecular reproduction and development

authors： Phillips KP,Baltz JM

更新日期：1996-09-01 00:00:00

abstract：:Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated t...

journal_title：Molecular reproduction and development

authors： Ivanova MM,Rosenkranz AA,Smirnova OA,Nikitin VA,Sobolev AS,Landa V,Naroditsky BS,Ernst LK

更新日期：1999-10-01 00:00:00

abstract：:The non-neuronal monoamine transporters (OCT1, OCT2, EMT, and PMAT) play a key role in the clearance of monoamines from extracellular compartments. In a previous report we described endometrial distribution and cyclic variation of the vesicular monoamine transporter (VMAT2) mRNA and the neuronal norepinephrine transpo...

journal_title：Molecular reproduction and development

authors： Bottalico B,Noskova V,Pilka R,Larsson I,Domanski H,Casslén B,Hansson SR

更新日期：2007-10-01 00:00:00

abstract：:Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...

journal_title：Molecular reproduction and development

authors： Magnani L,Cabot RA

更新日期：2007-10-01 00:00:00

abstract：:A high efficient and simple transgenic technology on mice and rabbits to transfect spermatozoa with exogenous DNA/DMSO complex to obtain transgenic offspring, which is namely called DMSO-sperm mediated gene transfer (SMGT). Mouse sperm could be either directly transfected via injection into testis or cultured in vitro...

journal_title：Molecular reproduction and development

authors： Shen W,Li L,Pan Q,Min L,Dong H,Deng J

更新日期：2006-05-01 00:00:00