Abstract:
:Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnRNA synthesis are arrested during three cell cycles following fusion. The activation of both appears during the eight-cell stage of the reconstructed embryo, after the same number of cell cycles after fusion as in nonmanipulated bovine embryos after fertilization. "Blebbing" activity of nuclear envelope, which is already absent in original blastomeres, reappears after fusion and continues for the next two cell cycles. From the present results, it can be concluded that the donor nuclei are arrested after fusion in morphology and function. Their reactivation corresponds to the developmental pattern typical for normal bovine embryos.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Kanka J,Fulka J Jr,Fulka J,Petr Jdoi
10.1002/mrd.1080290204subject
Has Abstractpub_date
1991-06-01 00:00:00pages
110-6issue
2eissn
1040-452Xissn
1098-2795journal_volume
29pub_type
杂志文章abstract::Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.1039
更新日期:2001-07-01 00:00:00
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2007-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20002
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pub_type: 杂志文章
doi:10.1002/mrd.20262
更新日期:2005-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20971
更新日期:2009-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199610)45:2<218::AID-MRD15
更新日期:1996-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199705)47:1<99::AID-MRD13>
更新日期:1997-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199905)53:1<84::AID-MRD10>
更新日期:1999-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23292
更新日期:2020-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23255
更新日期:2019-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370208
更新日期:1994-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080400206
更新日期:1995-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280210
更新日期:1991-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2003-04-01 00:00:00