Abstract:
:In the present study, immunogold labeling of ultrathin sections of ejaculated sperm was used to obtain insight into the ultrastructural localization and presumable function of type II cAMP-dependent protein kinase in sperm motion. In the flagellum, a human-specific isoform of the RII alpha subunit was located on the axonemal microtubule wall, whereas a different isoform of broader specificity was present in the cytoplasm at the periphery of the coarse fibers and fibrous sheath. This isoform was also found in the mitochondria. The human-specific RII alpha subunit is likely linked to microtubules by a unique binding protein of M(r) 72 kD. These findings are in agreement with the concept of a concerted mechanism involving phosphorylation of both the axonemal microtubules and the fibrous structures for the regulation of mammalian sperm motion.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Pariset C,Weinman Sdoi
10.1002/mrd.1080390410subject
Has Abstractpub_date
1994-12-01 00:00:00pages
415-22issue
4eissn
1040-452Xissn
1098-2795journal_volume
39pub_type
杂志文章abstract::This study was designed to examine the recovery of mitochondrial function and endogenous antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro maturation, bovine meiosis-II oocytes were vitrified, and then surviving oocytes were cultured an additional 8 hr. ATP content, ATP synt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21389
更新日期:2011-12-01 00:00:00
abstract::Spermatozoa from the sperm-rich fractions of the semen of 6 beagle dogs were capacitated and the effect of both zona pellucida (ZP) proteins and progesterone on calcium flux and the acrosome reaction measured. Sperm calcium flux was determined using the dual wavelength calcium probe indo-1/AM (6 microM) in a flow cyto...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1114
更新日期:2001-12-01 00:00:00
abstract::EPPIN (epididymal protease inhibitor; SPINLW1), an antimicrobial cysteine-rich protein containing both Kunitz and whey acidic protein (WAP)-type four disulfide core protease inhibitor consensus sequences, is a target for male contraception because of its critical role in sperm motility. Here, we characterized EPPIN's ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22119
更新日期:2012-12-01 00:00:00
abstract::Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20695
更新日期:2007-07-01 00:00:00
abstract::As a means of determining whether ovarian follicular fluid reaches the functional sperm reservoir in the caudal isthmus of the Fallopian tube shortly after ovulation, 0.01-0.02 ml aliquots of whole or steroid-free follicular fluid were introduced into the distal extremity of the isthmus within 1 hr before ovulation. E...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199911)54:3<283::AID-MRD9>
更新日期:1999-11-01 00:00:00
abstract::Increased oxidative stress is one of the main causes of poorly developed embryos in assisted reproductive technologies. Nicotinamide (NAM) has been shown to suppress reactive oxygen species (ROS) production through its potent antioxidative and anti-senescent effects. In the present study, we explored the effects of sh...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23417
更新日期:2020-10-01 00:00:00
abstract::Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23154
更新日期:2019-07-01 00:00:00
abstract::Ovulated rat oocytes undergo spontaneous activation during in vitro culture. After extrusion of the second polar body, they do not enter interphase but are arrested again in next metaphase-like stage (M III arrest). The present study demonstrates that puromycin and chloral hydrate can trigger transition to interphase ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280210
更新日期:1991-02-01 00:00:00
abstract::Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22686
更新日期:2016-09-01 00:00:00
abstract::We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199902)52:2<189::AID-MRD10
更新日期:1999-02-01 00:00:00
abstract::The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21233
更新日期:2010-10-01 00:00:00
abstract::The present study was undertaken to evaluate the effects of hyperstimulation and aging on the number and proportion of oocytes in the metaphase II stage in female Wistar rats. It explored the validity of the hypothesis that a combination of hyperstimulation with pregnant mare serum gonadotrophins (PMSG) and age could ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200001)55:1<104::AID-MRD14
更新日期:2000-01-01 00:00:00
abstract::Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200004)55:4<422::AID-MRD10
更新日期:2000-04-01 00:00:00
abstract::Previous work provided evidence of Na+/H+ exchanger activity in the apical domain of mouse trophectodermal plasma membranes that provides a route for entry of extracellular Na+ (Manejwala et al., 1989). This activity was hypothesized to contribute to the trans-trophectodermal Na+ flux that is required for blastocoel e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199806)50:2<146::AID-MRD4>
更新日期:1998-06-01 00:00:00
abstract::Heat shock proteins (HSPs) are known to play an important role not only in various stress conditions such as exposure to heat shock, but also in normal development and/or differentiation. The role of small heat shock proteins such as HSP25 in early embryo development remains largely unknown. In the present study, we e...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1125
更新日期:2002-01-01 00:00:00
abstract::Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20674
更新日期:2007-09-01 00:00:00
abstract::Rainbow trout (Oncorhynchus mykiss) oocytes were incubated for 3 hr in ovarian fluid alone (CC), or cortisol-enriched ovarian fluid [100 or 1,000 ng ml(-1) (CL and CH, respectively)], after which they were fertilized; the growth and development of the embryos reared from these oocytes was monitored until first feed, a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21239
更新日期:2010-10-01 00:00:00
abstract::In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199702)46:2<190::AID-MRD9>
更新日期:1997-02-01 00:00:00
abstract::In the mammalian testis the cytoplasmic beta and gamma actins are expressed in all stages of germ-cell differentiation, whereas gamma enteric actin is expressed in germ cells solely in postmeiotic stages. Northern blot analysis of mouse testicular RNAs reveals actin mRNAs of about 2.1, 1.5, and 1.4 kB. The 2.1-kB mRNA...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199606)44:2<141::AID-MRD1>
更新日期:1996-06-01 00:00:00
abstract::The aim of the present study was to investigate the implication of protein kinase C (PKC) in the mouse egg activation process. We used OAG (1-oleoyl-2-acetyl-sn-glycerol) as a PKC activator, calphostin C as a specific PKC inhibitor, and the calcium ionophore A23187 as a standard parthenogenetic agent. The exposure of ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199710)48:2<292::AID-MRD18
更新日期:1997-10-01 00:00:00
abstract::Embryonic stem (ES) cell lines are routinely derived from in vivo produced blastocysts. We investigated the efficiency of ES cells derivation from in vitro produced blastocysts either in monoculture or sequential culture. Zygotes from hybrid F1 B6D2 mice were cultured in vitro to the blastocyst stage in Potassium (K(+...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20512
更新日期:2006-08-01 00:00:00
abstract::The objective of this study was to examine the effects of colcemid treatment during oocyte in vitro maturation (IVM) and embryonic development after parthenogenetic activation (PA) and somatic-cell nucleus transfer (SCNT) in pigs. Immature oocytes were treated with colcemid from 0 to 22, 38 to 42, or 0 to 22 hr follow...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22498
更新日期:2015-06-01 00:00:00
abstract::Meiotic progression requires the translation of maternal mRNAs in a strict temporal order. In isolated animal oocytes, translation of maternal mRNAs containing a cytoplasmic polyadenylation element (CPE), such as cyclin B, is activated by in vitro stimulation of meiotic resumption which induces phosphorylation of CPEB...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22599
更新日期:2016-01-01 00:00:00
abstract::The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20916
更新日期:2009-01-01 00:00:00
abstract::Superovulated hamster oocytes were cryopreserved and thawed according to our carefully designed procedures. More than 90% (92 +/- 4%) of oocytes survived freezing and thawing. They were proven to be well conserved, showing excellent performance comparable to freshly ovulated oocytes in the human sperm penetration test...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330213
更新日期:1992-10-01 00:00:00
abstract::Rat epididymal glycoprotein DE associates with the dorsal region of the sperm head during sperm maturation, migrates to the equatorial segment (ES) with the acrosome reaction (AR), and is involved in gamete membrane fusion. In the present study we examined the association of DE with the sperm surface and the relations...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200006)56:2<180::AID-MRD9>
更新日期:2000-06-01 00:00:00
abstract::The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080340202
更新日期:1993-02-01 00:00:00
abstract::Nucleolar fine structure, "blebbing" activity of nuclear envelope, and activation of heterogeneous nuclear RNA (hnRNA) synthesis were studied in bovine reconstructed embryos obtained by electrofusion of a single eight-cell blastomere with an enucleated oocyte. Developmental progress of nucleolar fine structure and hnR...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080290204
更新日期:1991-06-01 00:00:00
abstract::Enzyme activities were determined quantitatively in individual rat oocytes to study their energy metabolism during maturation. Low hexokinase activity and high activities of lactate dehydrogenase and enzymes in the phosphate pathway, i.e., glucose 6-P and 6-P gluconate dehydrogenases, were characteristic of immature o...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080330315
更新日期:1992-11-01 00:00:00
abstract::The internally fertilizing primitive frog Ascaphus truei (family Ascaphidae) from the Pacific Northwest is the only frog with an intromittent organ. The more advanced neobatrachian frog Eleutherodactylus coqui (family Leptodactylidae) from Puerto Rico has secondarily acquired internal fertilization but mates by cloaca...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20207
更新日期:2005-03-01 00:00:00