Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa.

abstract：:We hypothesized that the testis-specific isoform of angiotensin-converting enzyme (tACE) is released during bovine sperm capacitation, and its peptidase activity is required for capacitation. Specific objectives of this study were to (i) develop an anti-tACE antibody; (ii) characterize expression of tACE in bovine tes...

journal_title：Molecular reproduction and development

authors： Ojaghi M,Kastelic J,Thundathil J

更新日期：2017-05-01 00:00:00

abstract：:A full-length cDNA encoding the bovine transforming growth factor beta (TGF-beta) receptor type I (bT beta R-I) was isolated from a placenta cDNA library. The deduced protein sequence of 499 residues contains a single transmembrane domain, a cysteinerich extracellular domain, and an intracellular kinase domain with pr...

journal_title：Molecular reproduction and development

authors： Roelen BA,Van Eijk MJ,Van Rooijen MA,Bevers MM,Larson JH,Lewin HA,Mummery CL

更新日期：1998-01-01 00:00:00

abstract：:Centrosome reduction during spermiogenesis has been studied using anti-gamma-tubulin and anti-centrin antibodies and electron microscopy in nonhuman primates. Rhesus spermatids possess apparently normal centrosomes comprising a pair of centrioles associated with gamma-tubulin and centrin. However, they do not nucleate...

journal_title：Molecular reproduction and development

authors： Manandhar G,Schatten G

更新日期：2000-08-01 00:00:00

abstract：:We report the primary structures of two mRNA species (GH1 and GH2), each predicted from the cloned cDNA and genomic gene sequences, that encode growth hormone in rainbow trout (Salmo gairdneri). Both GH1 and GH2 mRNA contain open reading frames comprising 630 nucleotides and encode 210 amino acid residues, of which 11...

journal_title：Molecular reproduction and development

authors： Agellon LB,Davies SL,Lin CM,Chen TT,Powers DA

更新日期：1988-01-01 00:00:00

abstract：:The expression of both inhibin alpha- and inhibin/activin beta A-subunit mRNA was examined in equine uteroplacental tissues collected during pregnancy (days 90 to 300). Northern blot analysis revealed that 5 transcripts (7.0, 4.1, 3.4, 2.6, 1.5 kb) of beta A-subunit were present, and the most abundantly expressed tran...

journal_title：Molecular reproduction and development

authors： Yamanouchi K,Hirasawa K,Hasegawa T,Ikeda A,Chang KT,Matsuyama S,Nishihara M,Miyazawa K,Sawasaki T,Tojo H,Tachi C,Takahashi M

更新日期：1997-08-01 00:00:00

abstract：:Eggs of Ascidia ceratodes and Phallusia mammillata block polyspermy by releasing a phosphatidylinositol-linked glycosidase from the follicle cell and egg surface that binds to and blocks all unoccupied sperm binding sites on the vitelline coat. Release of this glycosidase is thought to be under the control of a membra...

journal_title：Molecular reproduction and development

authors： Robert LK,Lucio-Gough LM,Goode CA,Mckinney K,Lambert CC

更新日期：1999-09-01 00:00:00

abstract：:The gonadotropin releasing hormone (GnRH) has long been considered as a neuropeptide involved in the control of the reproductive cycle. However, the presence of GnRH and its receptors in various tissues, including ovary and testis, suggests a role as autocrine/paracrine factor. In the present study, we report the expr...

journal_title：Molecular reproduction and development

authors： Uzbekova S,Ferrière F,Guiguen Y,Bailhache T,Breton B,Lareyre JJ

更新日期：2001-05-01 00:00:00

abstract：:Bicarbonate/CO2 is believed to be the key in vitro effector of sperm capacitation, a process which induces major changes in the sperm plasma membrane in preparation for fertilization. In a flow cytometric study, we examined the effect of bicarbonate on boar spermatozoa using merocyanine, an impermeant lipophilic probe...

journal_title：Molecular reproduction and development

authors： Harrison RA,Ashworth PJ,Miller NG

更新日期：1996-11-01 00:00:00

abstract：:It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (Gt...

journal_title：Molecular reproduction and development

authors： Hu X,Liu X,Zhang H,Zhang Y,Li S,Sang Q,Wang Q,Luo W,Liu Q,Lu D,Meng Z,Lin H

更新日期：2011-06-01 00:00:00

abstract：:Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

journal_title：Molecular reproduction and development

authors： Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

更新日期：2007-09-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00

abstract：:Smarca 2 (Brahma) and Smarca 4 (Brahma related gene 1, BRG1) alternatively occupy the catalytic site of SWI/SNF chromatin remodeling complexes. Mammalian embryos undergo a dramatic amount of epigenetic remodeling during cleavage development, which plays key roles in regulating both gene transcription and the developme...

journal_title：Molecular reproduction and development

authors： Magnani L,Cabot RA

更新日期：2007-10-01 00:00:00

abstract：:Studies have shown that aberrant expression of IL-12p40, which is encoded by the interleukin-12B (IL-12B) gene, may be involved in the development of endometriosis. In this study, we investigated the role of aberrant methylation of the IL-12B promoter region and its associated expression in the development of ovarian ...

journal_title：Molecular reproduction and development

authors： Zhao W,Kang S,Zhao J,Wang L,Cao S,Li Y

更新日期：2019-06-01 00:00:00

abstract：:A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...

journal_title：Molecular reproduction and development

authors： Holden CA,Hyne RV,Sathananthan AH,Trounson AO

更新日期：1990-03-01 00:00:00

abstract：:Limited understanding of the importance of known pluripotency factors in pig embryonic stem cells (ESC) impedes the establishment and validation of porcine ESC lines. This study evaluated the expression of known mouse ESC and human ESC (hESC) pluripotency markers in in vivo inner cell mass (ICM) and in vitro-cultured ...

journal_title：Molecular reproduction and development

authors： Blomberg LA,Schreier LL,Talbot NC

更新日期：2008-03-01 00:00:00

abstract：:cAMP response element binding protein (CREB)-binding protein (CBP) and p300 are two structurally related transcriptional co-activators that activate expression of many eukaryotic genes. Current dogma would suggest that these transcriptional co-activators have similar mechanisms of transcription regulation. Studies of ...

journal_title：Molecular reproduction and development

authors： Kwok RP,Liu XT,Smith GD

更新日期：2006-07-01 00:00:00

abstract：:Integrins are a family of cell adhesion receptors involved in many cell-cell and cell-matrix interactions. Some of these heterodimeric receptors, such as alpha 5 beta 1 and alpha v beta 1, specifically recognize the amino acid sequence Arg-Gly-Asp (RGD) within their ligands. The RGD sequence is found in fibronectin, v...

journal_title：Molecular reproduction and development

authors： Fusi FM,Vignali M,Gailit J,Bronson RA

更新日期：1993-10-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:The normal proto-oncogene c-fms encodes the macrophage growth factor (M-CSF) receptor involved in growth, survival, and differentiation along the monocyte-macrophage lineage of hematopoietic cell development. A major portion of our research concerns unraveling the temporal, molecular, and structural features that dete...

journal_title：Molecular reproduction and development

authors： Rohrschneider LR,Bourette RP,Lioubin MN,Algate PA,Myles GM,Carlberg K

更新日期：1997-01-01 00:00:00

abstract：:In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...

journal_title：Molecular reproduction and development

authors： Lee SG,Park CH,Choi DH,Kim HS,Ka HH,Lee CK

更新日期：2007-11-01 00:00:00

abstract：:In vitro culture of mammalian preimplantation embryos is associated with various developmental disorders such as retardation in development and cell damage. The molecular mechanisms underlying these processes are poorly understood. One of the possible reasons may be the unphysiologically high oxygen concentration used...

journal_title：Molecular reproduction and development

authors： Koerber S,Santos AN,Tetens F,Küchenhoff A,Fischer B

更新日期：1998-04-01 00:00:00

abstract：:An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression li...

journal_title：Molecular reproduction and development

authors： Moore A,Penfold LM,Johnson JL,Latchman DS,Moore HD

更新日期：1993-03-01 00:00:00

abstract：:Germ cells require communication with associated somatic cells for normal gametogenesis, as exemplified by an oocyte that interacts with granulosa cells via paracrine factors as well as gap junctions located at sites of contact between these two cell types. The objective of the present study was to define the mechanis...

journal_title：Molecular reproduction and development

authors： McGinnis LK,Kinsey WH

更新日期：2015-02-01 00:00:00

abstract：:Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase (cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetecti...

journal_title：Molecular reproduction and development

authors： Nuttinck F,Reinaud P,Tricoire H,Vigneron C,Peynot N,Mialot JP,Mermillod P,Charpigny G

更新日期：2002-01-01 00:00:00

abstract：:XIST is an X-linked, non-coding gene responsible for the cis induction of X-chromosome inactivation (XCI). Knockout of the XIST allele on an active X chromosome abolishes erroneous XCI and enhances the in vivo development of cloned mouse embryos by more than 10-fold. This study aimed to investigate whether a similar m...

journal_title：Molecular reproduction and development

authors： Yang Y,Wu D,Liu D,Shi J,Zhou R,He X,Quan J,Cai G,Zheng E,Wu Z,Li Z

更新日期：2017-06-01 00:00:00

abstract：:Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...

journal_title：Molecular reproduction and development

authors： Sim BW,Cha JJ,Song BS,Kim JS,Yoon SB,Choi SA,Jeong KJ,Kim YH,Huh JW,Lee SR,Kim SH,Lee CS,Kim SU,Chang KT

更新日期：2013-03-01 00:00:00

abstract：:The mussel Mytilus edulis can be used as model to study the molecular basis of reproductive isolation because this species maintains its species integrity, despite of hybridizing in zones of contact with the closely related species M. trossulus or M. galloprovincialis. This study uses selective antibody production by ...

journal_title：Molecular reproduction and development

authors： Stuckas H,Messerschmidt K,Putzler S,Baumann O,Schenk JA,Tiedemann R,Micheel B

更新日期：2009-01-01 00:00:00

abstract：:In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...

journal_title：Molecular reproduction and development

authors： Cabot B,Tseng YC,Crodian JS,Cabot R

更新日期：2017-12-01 00:00:00

abstract：:There are five methyl binding domain (MBD) proteins characterized by a methyl CpG-binding domain. Four MBD proteins (MeCP2 and MBDs 1-3) are linked to transcriptional repression and one (MBD4), to DNA repair. During preimplantation development, the embryo undergoes global demethylation following fertilization and sele...

journal_title：Molecular reproduction and development

authors： Ruddock-D'Cruz NT,Xue J,Wilson KJ,Heffernan C,Prashadkumar S,Cooney MA,Sanchez-Partida LG,French AJ,Holland MK

更新日期：2008-01-01 00:00:00

abstract：:Clusterin is a heterodimeric glycoprotein synthesized and secreted by rat Sertoli cells and epididymal epithelium. The goal of this study was to determine the presence of clusterin in the luminal fluid of the cauda epididymides and its association with the membranes of developing spermatozoa in the presence and absenc...

journal_title：Molecular reproduction and development

authors： Mattmueller DR,Hinton BT

更新日期：1992-05-01 00:00:00