Abstract:
:An antiserum, designated R4 and raised against denatured hamster acrosomes, was shown to localize specifically to the acrosomal region of hamster, rat, mouse, and human spermatozoa, and to inhibit both hamster and human sperm-oocyte binding in vitro. Following screening of a human testis lambda gt11 cDNA expression library with the antiserum R4, a series of cDNA clones were isolated. One (cDNA 134) was selected based on the ability of the beta-galactosidase fusion protein to inhibit human and hamster sperm-zona binding in vitro. The fusion protein was also shown to inhibit the penetration of zona-free hamster oocytes by human spermatozoa. Sequence analysis revealed that cDNA 134 coded for a portion of a serine protease inhibitor (serpin) closely related to plasma Protein C inhibitor. Sequencing of an additional cDNA clone (261) and Northern blot analysis confirmed that a Protein C inhibitor-like mRNA is synthesised in the human testis. Affinity-purified anti-134 antibody specifically localized to the acrosomal region of both hamster and human sperm. Synthetic peptides corresponding to the conserved core region responsible for the interaction of the serpin with its cognate protease also blocked human sperm-zona binding in vitro. The results suggest that this acrosomally located inhibitor plays an important role in the series of binding events that results in human fertilization.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Moore A,Penfold LM,Johnson JL,Latchman DS,Moore HDdoi
10.1002/mrd.1080340308subject
Has Abstractpub_date
1993-03-01 00:00:00pages
280-91issue
3eissn
1040-452Xissn
1098-2795journal_volume
34pub_type
杂志文章abstract::Spermiogenesis pursues three major objectives: (1) The safeguard of the male genome within the confines of a compact nucleus. (2) The accumulation of enzymes in the acrosome of be released at fertilization. (3) The development of a sperm propelling tail consisting of an axoneme surrounded by a scaffold of keratin-cont...
journal_title:Molecular reproduction and development
pub_type: 评论,杂志文章
doi:10.1002/mrd.1040
更新日期:2001-08-01 00:00:00
abstract::The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...
journal_title:Molecular reproduction and development
pub_type: 传,历史文章,杂志文章
doi:10.1002/mrd.21087
更新日期:2009-10-01 00:00:00
abstract::We previously demonstrated that the progesterone-(P) initiated human sperm acrosome reaction (AR) was dependent on the presence of extracellular Na+ (Na(-)0). Moreover, Na(-)0 depletion resulted in a decreased cytosolic pH (pHi), suggesting involvement of a Na(+)-dependent pHi regulatory mechanism during the P-initiat...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199902)52:2<189::AID-MRD10
更新日期:1999-02-01 00:00:00
abstract::Semen analysis lacks a functional component and best identifies extreme cases of infertility. The ganglioside GM1 is known to have functional roles during capacitation and acrosome exocytosis. Here, we assessed whether GM1 localization patterns (Cap-Score™) correspond with male fertility in different settings: Study 1...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22803
更新日期:2017-05-01 00:00:00
abstract::Cattle consuming endophyte-infected tall fescue grass have an associated reduction in circulating progesterone and reduced reproductive rates. In this study, commercially available rat microarrays were used to analyze the gene expression in luteal tissues from heifers fed endophyte-free fescue, endophyte-infected fesc...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10395
更新日期:2004-02-01 00:00:00
abstract::We have previously shown that bovine oocytes parthenogenetically activated after 40 hours (hr) of in vitro maturation proceed through the cell cycle faster than those after 20 hr of maturation. In the present study, we used this model of different speed of nuclear progression to investigate the correlation of two hall...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10072
更新日期:2002-05-01 00:00:00
abstract::In this review, we discuss the expression, regulation, downstream mechanisms, and function of stress-induced stress enzymes in mammalian oocytes, peri-implantation embryos, and the stem cells derived from those embryos. Recent reports suggest that stress enzymes mediate developmental functions during early mammalian d...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.20787
更新日期:2008-04-01 00:00:00
abstract::Successful fertilization in mammals depends on the sperm's ability to initiate intracellular Ca2+ oscillations in the egg, a phenomenon that is elicited by Sperm-oocyte activating factors (SOAFs), whose quantitative and/or qualitative defect might result in fertilization failure. One such proposed factor is Post-acros...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22950
更新日期:2018-02-01 00:00:00
abstract::We have previously described a hamster sperm glycoprotein, P26h, which is implicated in the cascade of events occurring during the interaction between mature spermatozoa and the oocyte's zona pellucida. The P26h is acquired on the acrosomal cap of the spermatozoon during its maturation arising within the epididymis. L...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1050
更新日期:2001-08-01 00:00:00
abstract::Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20182
更新日期:2005-01-01 00:00:00
abstract::One of the hallmarks of mammalian sperm capacitation is the loss of cholesterol from the plasma membrane. Cholesterol has been associated with the formation of detergent insoluble membrane microdomains in many cell types, and sperm from several mammalian species have been shown to contain detergent-resistant membranes...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20540
更新日期:2006-12-01 00:00:00
abstract::During implantation in mice, tissue inhibitor of matrix metalloproteinases-3 is believed to play a key role in inhibiting matrix metalloproteinase activity associated with embryo invasion and tissue remodeling. The first objective of this study was to quantitatively compare the steady-state mRNA levels of tissue inhib...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1018
更新日期:2001-06-01 00:00:00
abstract::Under the influence of progesterone, the porcine uterus synthesizes plasmin/trypsin inhibitor (PTI), a low molecular weight protein (M(r) approximately 14,000) belonging to the Kunitz family of proteinase inhibitors. Here it is demonstrated that mRNA for the same protein is produced by the developing trophoblast durin...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199704)46:4<443::AID-MRD1>
更新日期:1997-04-01 00:00:00
abstract::Bicarbonate/CO2 is believed to be the key in vitro effector of sperm capacitation, a process which induces major changes in the sperm plasma membrane in preparation for fertilization. In a flow cytometric study, we examined the effect of bicarbonate on boar spermatozoa using merocyanine, an impermeant lipophilic probe...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199611)45:3<378::AID-MRD16
更新日期:1996-11-01 00:00:00
abstract::Data from our laboratory have shown that an estrogen (E2)-dependent M(r) 90,000-92,000 protein and its mRNA are synthesized and expressed in abundant amounts at estrus from the fimbria and ampulla, not isthmus, oviduct of the sheep. Immunocytochemical studies have shown that the M(r) 90,000-92,000 protein is contained...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420303
更新日期:1995-11-01 00:00:00
abstract::The neomycin-resistant gene (neo(r)) is probably the most commonly used selectable marker gene in gene targeting and gene transfection research. In this study, the neo(r) gene construct was introduced into in vitro cultured goat foetal fibroblast cells (IV-5), and the cells were selected with 900 microg/ml G418. The G...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1143
更新日期:2002-02-01 00:00:00
abstract::Post-acrosomal WW-domain binding protein (PAWP) is a signaling molecule located in the post-acrosomal sheath (PAS) of mammalian spermatozoa. We hypothesized that the proper integration of PAWP in the sperm PAS is reflective of bull-sperm quality and fertility. Cryopreserved semen samples from 298 sires of acceptable, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22309
更新日期:2014-05-01 00:00:00
abstract::Intracytoplasmic sperm injection (ICSI) is an important assisted reproductive technology (ART). Due to deployment difficulties and low efficiency of the earlier (conventional) version of ICSI, especially in the mouse, a piezo-assisted ICSI technique had evolved as a popular ART methodology in recent years. An importan...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20919
更新日期:2008-12-01 00:00:00
abstract::The purposes of this study were to establish and to explore the biological basis of the chronic stress-induced premature ovarian failure (POF) model and to explore the therapeutic effects of the traditional Chinese medicine Muniziqi. Sexually matured female Sprague-Dawley rats were fed with spinach and cilantro in col...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23092
更新日期:2019-02-01 00:00:00
abstract::Normal epididymal function, such as protein expression and secretion, is primarily regulated by testicular androgens and temperature. However, the role of spermatozoa in this critical process has never been studied. In order to determine whether sperm itself could regulate epididymal function, we have developed a cell...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20751
更新日期:2008-03-01 00:00:00
abstract::The formation of cell membrane following CSF-1 stimulation of a macrophage cell line is coordinated with cell cycle progression. The majority of membrane phospholipid accumulates during the S phase and results from cell-cycle dependent oscillations in the rates of phosphatidylcholine biosynthesis and degradation. Both...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/(SICI)1098-2795(199701)46:1<24::AID-MRD5>3
更新日期:1997-01-01 00:00:00
abstract::Successful pregnancy depends on the precise regulation of extravillous trophoblast cell invasion ability. MicroRNA-210-3p (miR-210), which is increased in the placenta of pre-eclampsia. Furthermore, miR-210 could inhibit trophoblasts invasion and might act as a serum biomarker for pre-eclampsia. Previous studies have ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23154
更新日期:2019-07-01 00:00:00
abstract::Changes in the level of transcriptional activity in 32-cell stage morula nuclei were studied after blastomere electrofusion to enucleated oocytes. Nuclear transplant recipients were pulse labelled with 3H-uridine during cultivation in vitro, embryos were then fixed and processed for autoradiography and electron micros...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199602)43:2<135::AID-MRD1>
更新日期:1996-02-01 00:00:00
abstract::This study was carried out to examine the participation of epidermal growth factor (EGF)-like peptides in the induction of germinal vesicle breakdown (GVB) in mouse cumulus cell-enclosed oocytes (CEO). The EGF-like peptide, amphiregulin (AR), dose-dependently stimulated meiotic resumption in CEO, but not denuded oocyt...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20781
更新日期:2008-01-01 00:00:00
abstract::Transcription factors inhibit or assist RNA polymerases in the initiation and maintenance of transcription; however, the cell specific expression and roles of transcription factors within bovine ovarian follicles during development are unknown. The aim of present study was to determine if the expression of transcripti...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20819
更新日期:2008-05-01 00:00:00
abstract::Oocyte maturation is an important process required to achieve optimal oocyte quality, and later affects fertilization potential and subsequent embryo development. The maturation process includes synchronized nuclear and cytoplasmic remodeling, in which cytoskeletal and centrosome dynamics play an important role and si...
journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
doi:10.1002/mrd.21380
更新日期:2011-10-01 00:00:00
abstract::Pannexins (Panxs) are channel-forming proteins that have homology to the invertebrate gap junction proteins, the innexins. These proteins form membrane channels implicated in ATP release. To evaluate the role of Panxs in the male reproductive tract, we investigated the distribution and regulation of Panx1 and 3 in the...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21280
更新日期:2011-02-01 00:00:00
abstract::In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20501
更新日期:2007-07-01 00:00:00
abstract::Maternal immune tolerance to the semi-allogenic fetus is required for successful pregnancy in mammals. Monoclonal nonspecific suppressor factor beta (MNSFB) is an immunosuppressive factor present in uterine epithelial and stromal cells, as well as in macrophages and T cells. Although the functional neutralization of M...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22495
更新日期:2015-06-01 00:00:00
abstract::The objective of this study was to examine the effect of treating pig oocytes during in vitro maturation (IVM) with a proteasome inhibitor, MG132, on oocyte maturation and embryonic development. In one series of experiments, oocytes from medium-sized follicles (3-8 mm in diameter) were untreated (MCO) or treated with ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21402
更新日期:2012-01-01 00:00:00