Abstract:
:Pannexins (Panxs) are channel-forming proteins that have homology to the invertebrate gap junction proteins, the innexins. These proteins form membrane channels implicated in ATP release. To evaluate the role of Panxs in the male reproductive tract, we investigated the distribution and regulation of Panx1 and 3 in the testis, efferent ducts (ED), and epididymis of adult rats. In the testis, Panx1 localized to the basal compartment of the seminiferous epithelium, while Panx3 was expressed in Leydig cells. In the ED, both Panxs were expressed in the apical region of ciliated cells. In the epididymis, Panx1 was detected at the base of the epithelium, at times encompassing basal cells, while Panx3 was restricted to the apical plasma membrane of principal cells. Panx3 immunoreactions were high throughout the entire epididymis while Panx1 was high in all regions except the initial segment. Multiple transcripts for Panx1 were identified, and sequence analysis indicated that alternative splicing might account for them. Orchidectomy resulted in the expression of multiple immunoreactive Panx1 bands, and these appeared to be androgen-repressed throughout the epididymis. Panx3 levels in all epididymal regions were also androgen-repressed. Deglycosylation experiments indicated that some Panx1 species were due to glycosylation, but this did not account for all Panx1 immunoreactive species. In summary, Panxs expressed in the epididymis and regulated by both alternative splicing events and androgens. These proteins may play a role in ATP secretion into the epididymal lumen and basal extracellular spaces for functions involving sperm transport and maturation.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Turmel P,Dufresne J,Hermo L,Smith CE,Penuela S,Laird DW,Cyr DGdoi
10.1002/mrd.21280subject
Has Abstractpub_date
2011-02-01 00:00:00pages
124-38issue
2eissn
1040-452Xissn
1098-2795journal_volume
78pub_type
杂志文章abstract::Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization-induced increase in intracellular calcium concentration ([Ca2+]i). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca2+]i did not change. Although eggs fertili...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080410218
更新日期:1995-06-01 00:00:00
abstract::The developmental competence of bovine follicular oocytes that had been meiotically arrested with the phosphokinase inhibitor 6-dimethylaminopurine (6-DMAP) was studied. After 24 h in vitro culture with 2 mM 6-DMAP, 85 +/- 12% of the oocytes were at the germinal vesicle stage compared to 97 +/- 3% at the start of cult...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199807)50:3<334::AID-MRD10
更新日期:1998-07-01 00:00:00
abstract::Proteins synthesized by DDK mice embryos were analyzed by 2D electrophoresis and a new egg-specific polypeptide, D14, was identified. The protein is characterized by its high rate of synthesis and electrophoretic properties (MW 36,500, pl greater than 8). The synthesis of D14 is strictly developmentally regulated: sta...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280303
更新日期:1991-03-01 00:00:00
abstract::Binding of neurotrophic ligands to rabbit spermatozoa was studied. Nicotinic cholinergic antagonists, [3H]alpha-bungarotoxin and [3H]dihydro-beta-erythroidine (DE), bound with high affinity to different sites in the tails of rabbit spermatozoa with the former binding to 10,207 sites/cell and the latter to 562 sites/ce...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280109
更新日期:1991-01-01 00:00:00
abstract::The fibrous sheath is a major cytoskeletal structure in the principal piece of the mammalian sperm flagellum. Two peptide sequences obtained from a tryptic digest of mouse fibrous sheath proteins exhibited high homology with mu-class glutathione S-transferases (GSTs). Using a DNA probe amplified from degenerate polyme...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420407
更新日期:1995-12-01 00:00:00
abstract::Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1029
更新日期:2001-07-01 00:00:00
abstract::A method for assessment of the human sperm acrosome reaction is reported using fluorescein isothiocyanate (FITC)-conjugated Concanavalin A (ConA). The technique involved labelling prefixed spermatozoa, where only those spermatozoa that showed a complete loss of the acrosome bound FITC-ConA to the acrosomal region. Com...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080250306
更新日期:1990-03-01 00:00:00
abstract::Tcp-11 is a candidate for a distorter gene within the t-complex on mouse chromosome 17; although t-complex genes appear to affect sperm function, relatively little is known about mechanisms whereby these genes might play a specific physiological role. We present evidence that the protein TCP-11 is found on the surface...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199711)48:3<375::AID-MRD11
更新日期:1997-11-01 00:00:00
abstract::The sperm nucleus has a unique chromatin structure where the DNA is highly condensed and associated with specific proteins, the protamines. It is a nondividing cell which is also transcriptionally inactive. After fusion with an oocyte, the sperm nucleus undergoes decondensation and, in the same time, starts replicatio...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200003)55:3<307::AID-MRD9>
更新日期:2000-03-01 00:00:00
abstract::Differential expression of genes leads to variations in the phenotypes of X and Y sperm, although some differentially expressed gene products are shared through intercellular bridges. Genes differentially expressed in bovine X and Y sperm were identified by a combination of suppression subtractive hybridization (SSH),...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22386
更新日期:2014-10-01 00:00:00
abstract::Carp hyosophorin (HSP) is purified from oocytes. It is a highly glycosylated protein (10% protein and 90% carbohydrate) of high molecular weight (>100 kDa) and is localized in the cortical granules of oocytes. During cortical reaction carp HSP is exocytosed into the perivitelline space and is rapidly cleaved to the lo...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199903)52:3<288::AID-MRD6>
更新日期:1999-03-01 00:00:00
abstract::To establish reliable criteria for the evaluation of nuclear donor embryos, we studied the effect of cell number and cell size of in vitro produced day 6 donor morulae on the rate of blastocyst formation following nuclear transfer to in vitro matured oocytes. In experiment 1, donor embryos were divided into three grou...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080420107
更新日期:1995-09-01 00:00:00
abstract::The objective of this study was to use atomic force microscopy (AFM), with submicron resolution, for morphophologic and morphometric analyses of acrosome intact and acrosome-reacted human sperm heads. A mixed population of acrosome intact and reacted sperm was produced by treating capacitated sperm with A23187, which ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10195
更新日期:2002-12-01 00:00:00
abstract::Estrogen receptor mRNA expression was detected in the head and tail parts of the human epididymis by means of reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical labelling showed that ciliate and nonciliate cells from the epithelium of the efferent ductules possessed strong to moderate nuclear...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199708)47:4<448::AID-MRD12
更新日期:1997-08-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) regulates folliculogenesis in the ovary and spermatogenesis in the testis via specific, high affinity membrane-bound receptors (FSHR). To assess the role of FSHR gene expression in regulating expression of FSHR protein in the plasma membrane, the effects of a porcine FSHR cDNA antise...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10310
更新日期:2003-06-01 00:00:00
abstract::Connexin 43 (Cx43), a gap junction protein expressed in differentiated granulosa cells, is necessary for normal follicular development. Cx43 expression and regulation by epidermal growth factor (EGF) were characterized in immature rabbit granulosa cells. Cx43 mRNA was expressed in the granulosa cells of primary follic...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10219
更新日期:2003-01-01 00:00:00
abstract::The present study was conducted to reveal effects of in ovo injection of nonsteroidal aromatase inhibitor (Fadrozole) or estradiol at day 3 of incubation on mRNA levels of P45017alphahydroxylase (P450c17), P450 aromatase (P450arom) and anti-Müllerian hormone (AMH) in the chicken gonads. The mRNA levels in the gonads a...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200001)55:1<20::AID-MRD4>3
更新日期:2000-01-01 00:00:00
abstract::Demecolcine-induced enucleation (IE) of mouse oocytes has been shown to improve development to term of cloned mice. In this study, we characterized the kinetics and morphological progression of bovine oocytes subjected to IE, and evaluated their ability to support embryo development to the blastocyst stage after nucle...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20356
更新日期:2005-10-01 00:00:00
abstract::The present study was designed to determine the effects of vitrification on promoter methylation and the expression levels of pluripotency and differentiation genes in mouse blastocysts. Promoter region CpG methylation patterns and the expression levels of octamer-binding transcription factor (Oct4), Nanog homeobox (N...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22052
更新日期:2012-07-01 00:00:00
abstract::In this study, we document that the overall rate of protein synthesis decreases during in vitro maturation (IVM) of pig oocytes despite enhanced formation of the 5' cap structure eIF4F. Within somatic/interphase cells, formation of the eIF4F protein complex correlates very well with overall rates of protein translatio...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20368
更新日期:2006-01-01 00:00:00
abstract::PLAC1 is a recently described, trophoblast-specific gene that localizes to a region of the X-chromosome important in placental development. Immunohistochemical analysis demonstrated that PLAC1 polypeptide localizes to the differentiated syncytiotrophoblast throughout gestation (8-41 weeks) as well as a small populatio...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20272
更新日期:2005-07-01 00:00:00
abstract::Glutamine (GLN) is a metabolic precursor for hexosamine synthesis and its inclusion in culture medium has been reported to improve cumulus expansion. Glutamine and cysteine share the same transport system. Excess external GLN may act as a competitive inhibitor for the uptake of cysteine and stimulate loss of cellular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199809)51:1<76::AID-MRD9>3
更新日期:1998-09-01 00:00:00
abstract::Pre-pubertal pig oocytes display reduced developmental competence compared with adult oocytes following in vitro maturation (IVM). Exposure to dibutyryl cyclic adenosine monophosphate (dbcAMP) for the first 20 hr IVM improves development of pre-pubertal oocytes, suggesting that their cAMP content may be inadequate. Th...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20555
更新日期:2006-10-01 00:00:00
abstract::The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/1098-2795(200012)57:4<323::AID-MRD3>3.0.CO
更新日期:2000-12-01 00:00:00
abstract::Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199604)43:4<484::AID-MRD11
更新日期:1996-04-01 00:00:00
abstract::Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22307
更新日期:2014-05-01 00:00:00
abstract::Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21224
更新日期:2010-09-01 00:00:00
abstract::The present study was undertaken to evaluate the effects of hyperstimulation and aging on the number and proportion of oocytes in the metaphase II stage in female Wistar rats. It explored the validity of the hypothesis that a combination of hyperstimulation with pregnant mare serum gonadotrophins (PMSG) and age could ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(200001)55:1<104::AID-MRD14
更新日期:2000-01-01 00:00:00
abstract::Testis induction is associated with gonadal Sry and Sox9 expression in mammals, and with Sox9 expression in vertebrates where Sry is absent. In mammals, Sry might initiate testis induction by upregulating Sox9 expression; however, direct evidence supporting this hypothesis is lacking. Models of Sry-negative XX sex rev...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.10317
更新日期:2003-08-01 00:00:00
abstract::In poikilothermic vertebrates, sex determination is sometimes influenced by environmental factors such as temperature. However, little is known about the molecular mechanisms underlying environmental sex determination. The medaka (Oryzias latipes) is a teleost fish with an XX/XY sex determination system. Recently, it ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.21203
更新日期:2010-08-01 00:00:00