Abstract:
:In poikilothermic vertebrates, sex determination is sometimes influenced by environmental factors such as temperature. However, little is known about the molecular mechanisms underlying environmental sex determination. The medaka (Oryzias latipes) is a teleost fish with an XX/XY sex determination system. Recently, it was reported that XX medaka can be sex-reversed into phenotypic males by high water temperature (HT; 32-34 degrees C) treatment during the sex differentiation period. Here we report that cortisol caused female-to-male sex reversal and that metyrapone (an inhibitor of cortisol synthesis) inhibited HT-induced masculinization of XX medaka. HT treatment caused elevation of whole-body levels of cortisol, while metyrapone suppressed the elevation by HT treatment during sexual differentiation. Moreover, cortisol and 33 degrees C treatments inhibited female-type proliferation of germ cells as well as expression of follicle-stimulating hormone receptor (fshr) mRNA in XX medaka during sexual differentiation. These results strongly suggest that HT induces masculinization of XX medaka by elevation of cortisol level, which, in turn, causes suppression of germ cell proliferation and of fshr mRNA expression.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Hayashi Y,Kobira H,Yamaguchi T,Shiraishi E,Yazawa T,Hirai T,Kamei Y,Kitano Tdoi
10.1002/mrd.21203subject
Has Abstractpub_date
2010-08-01 00:00:00pages
679-86issue
8eissn
1040-452Xissn
1098-2795journal_volume
77pub_type
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