Effect of oocyte maturation medium on in vitro development of in vitro fertilized bovine embryos.

Abstract:

:The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization. Oocytes were aspirated from 2-5 mm follicles of ovaries collected at a local abattoir. The oocyte-cumulus complexes (OCCs) were cultured for 23-25 h in one of seven commercially available media supplemented with 6 mg/ml bovine serum albumin (BSA), 0.25 mM pyruvate, 10 micrograms/ml luteinizing hormone (LH), 0.5 microgram/ml follicle-stimulating hormone (FSH), and 1 microgram/ml estradiol. After maturation for 23-25 h, all eggs were subjected to the same in vitro fertilization protocol using modified TALP medium and subsequently cultured in the same serum-free embryo culture medium (HECM-1/BSA) for 8 days, after which embryo development was assessed. Five media (SFRE, MEM alpha, TCM199, MEM alpha/+, RPMI:MEM alpha) better supported normal oocyte maturation as determined by embryo development to the two-cell (76-82%), morula/blastocyst (25-32%), and blastocyst (12-19%) stages. Oocytes that were matured in Waymouth's medium MB 752/l or Ham's F-12 had a significantly reduced incidence of cleavage to the two-cell stage (52% and 37%, respectively), which was not attributed to failure of fertilization. Of the eggs that did cleave to the two-cell stage in these two media, 27% and 9% developed to morulae/blastocysts but only 6% and 3%, respectively, developed into blastocysts.(ABSTRACT TRUNCATED AT 250 WORDS)

journal_name

Mol Reprod Dev

authors

Rose TA,Bavister BD

doi

10.1002/mrd.1080310113

keywords:

subject

Has Abstract

pub_date

1992-01-01 00:00:00

pages

72-7

issue

1

eissn

1040-452X

issn

1098-2795

journal_volume

31

pub_type

杂志文章
  • Molecular cloning and characterization of a mouse nuclear orphan receptor expressed in embryos and testes.

    abstract::A mouse cDNA encoding a putative DNA-binding protein of the zinc-finger type was isolated from an E8.5 mouse embryonic cDNA library. Sequence comparison revealed a high degree of homology between this mouse cDNA and the human and rat orphan receptor Tr2-11 isolated from prostate cDNA libraries. This transcript was det...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199607)44:3<305::AID-MRD4>

    authors: Lee CH,Chang L,Wei LN

    更新日期:1996-07-01 00:00:00

  • Five azacytidine, a DNA methyltransferase inhibitor, specifically inhibits testicular cord formation and Sertoli cell differentiation in vitro.

    abstract::In mammals, Sry, Sox9, and M33 act as regulators at a chromatin level and promote the maturation of embryonic gonads into testes. Recently, it was shown that transcriptional regulation by DNA methylation plays crucial roles in gene expression during the differentiation and development of various cell types. To determi...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20850

    authors: Mizukami T,Kanai Y,Fujisawa M,Kanai-Azuma M,Kurohmaru M,Hayashi Y

    更新日期:2008-06-01 00:00:00

  • Effects of thiol compounds on in vitro maturation of canine oocytes collected from different reproductive stages.

    abstract::Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20674

    authors: Hossein MS,Kim MK,Jang G,Oh HJ,Koo O,Kim JJ,Kang SK,Lee BC,Hwang WS

    更新日期:2007-09-01 00:00:00

  • Stage-specific expression of rat transition protein 2 mRNA and possible localization to the chromatoid body of step 7 spermatids by in situ hybridization using a nonradioactive riboprobe.

    abstract::The present study has used methoxyacetic acid (MAA)-induced depletion of specific germ cell types in the rat and in situ hybridization with nonradioactive riboprobes to determine the stages of the spermatogenic cycle at which there is expression of the mRNA for the basic chromosomal protein transition protein 2 (TP2)....

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080330404

    authors: Saunders PT,Millar MR,Maguire SM,Sharpe RM

    更新日期:1992-12-01 00:00:00

  • Birth of correctly genotyped calves after multiplex marker detection from bovine embryo microblade biopsies.

    abstract::We report a method for multiplex genotyping of bovine embryo microblade biopsies. We have tested the reliability of the method and the viability of the embryos in vitro and in vivo. Two polymorphic gene markers (GHR F279Y and PRLR S18N) associated with milk production traits and one marker for sex diagnosis (ZFX/ZFY) ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20731

    authors: Peippo J,Viitala S,Virta J,Räty M,Tammiranta N,Lamminen T,Aro J,Myllymäki H,Vilkki J

    更新日期:2007-11-01 00:00:00

  • Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline.

    abstract::Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.23062

    authors: Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

    更新日期:2018-12-01 00:00:00

  • Directional differentiation of chicken primordial germ cells into adipocytes, neuron-like cells, and osteoblasts.

    abstract::Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.21224

    authors: Li BC,Tian ZQ,Sun M,Xu Q,Wang XY,Qin YR,Xu F,Gao B,Wang KH,Sun HC,Chen GH

    更新日期:2010-09-01 00:00:00

  • Sperm storage in the female reproductive tract of Scotophilus heathii: role of androgen.

    abstract::The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.21328

    authors: Roy VK,Krishna A

    更新日期:2011-07-01 00:00:00

  • Effects of heterologous hematopoietic cytokines on in vitro differentiation of cultured porcine inner cell masses.

    abstract::An exogenous supply of hematopoietic cytokines is essential for maintaining murine embryonic stem (ES) cells in a proliferative yet undifferentiated state. Recently, it was demonstrated that hematopoietic cytokines utilize the gp130 signal transduction pathway to maintain this phenotype, although their involvement tow...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199610)45:2<139::AID-MRD5>

    authors: Moore K,Piedrahita JA

    更新日期:1996-10-01 00:00:00

  • OVGP1 is expressed in the canine oviduct at the time and place of oocyte maturation and fertilization.

    abstract::In the dog, oocyte maturation, fertilization, and early embryo development take place within the oviduct in the presence of increasing circulating progesterone (P4) levels. Expression of the oviduct-specific glycoprotein 1 (OVGP1), known in other species to be estrogen-dependent, was explored by real-time quantitative...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22417

    authors: Saint-Dizier M,Marnier C,Tahir MZ,Grimard B,Thoumire S,Chastant-Maillard S,Reynaud K

    更新日期:2014-11-01 00:00:00

  • Lipopolysaccharide-induced expression of FAS ligand in cultured immature boar sertoli cells through the regulation of pro-inflammatory cytokines and miR-187.

    abstract::Lipopolysaccharide (LPS) induces germ cell apoptosis, but its mechanism of action is not clear. One possibility is that LPS regulates the expression of FAS ligand (FASLG) in Sertoli cells, which will then influence germ cell apoptosis. In this study, LPS reduced the viability of cultured, immature boar Sertoli cells i...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22534

    authors: Wang Y,Zhang JJ,Yang WR,Luo HY,Zhang JH,Wang XZ

    更新日期:2015-11-01 00:00:00

  • Changes in exposed membrane proteins during in vitro capacitation of boar sperm.

    abstract::Exposed plasma membrane proteins were labeled with 125I before and after incubation of boar sperm under capacitating conditions. Labeled protein profiles were compared to the ability of the sperm to penetrate zona-free hamster ova. Quantitatively, the labeled sperm membrane proteins were primarily low Mr prior to capa...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080270311

    authors: Berger T

    更新日期:1990-11-01 00:00:00

  • Coactivator CBP in the regulation of conceptus IFNtau gene transcription.

    abstract::Studies of ovine interferon-tau (oIFNtau) gene regulation, an anti-luteolytic factor produced by conceptuses of the ruminant ungulates, have been carried out, but a definitive mechanism for its spatial-temporal transcription has not been elucidated. Recently, specific binding regions for transcription factors AP-1 and...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10293

    authors: Xu N,Takahashi Y,Matsuda F,Sakai S,Christenson RK,Imakawa K

    更新日期:2003-05-01 00:00:00

  • Differential expression of key subunits of SWI/SNF chromatin remodeling complexes in porcine embryos derived in vitro or in vivo.

    abstract::In vitro embryo production is an established method for both humans and animals, but is fraught with inferior development and health issues in offspring born after in vitro fertilization procedures. Analysis of epigenetic changes caused by exposure to in vitro conditions should shed light on potential sources of these...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22922

    authors: Cabot B,Tseng YC,Crodian JS,Cabot R

    更新日期:2017-12-01 00:00:00

  • A study of the nature of embryonic lethality in LIS1-/- mice.

    abstract::Homozygous deletion of the Lis1 gene (Lis1(-/-)) in mouse resulted in early embryonic lethality immediately after embryo implantation by an undefined mechanism. We seek to define the nature of this demise. LIS1 (pafah1b1) is a 46 kDa protein with seven tryptophan-aspartate (WD) repeats. It docks with many proteins and...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10339

    authors: Cahana A,Jin XL,Reiner O,Wynshaw-Boris A,O'Neill C

    更新日期:2003-10-01 00:00:00

  • Binding of nickel to testicular glutamate-ammonia ligase inhibits its enzymatic activity.

    abstract::Exposure to nickel has been shown to cause damage to the testis in several animal models. It is not known if the testis expresses protein(s) that can bind nickel. To test this, we used a nickel-binding assay to isolate testicular nickel-binding proteins. We identified glutamate-ammonia ligase (GLUL) as a prominent nic...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.21275

    authors: Sun Y,Ou Y,Cheng M,Ruan Y,van der Hoorn FA

    更新日期:2011-02-01 00:00:00

  • Fine structure of the germinating cells during the spermiogenesis of Arion rufus (Mollusca, Pulmonate Gastropoda).

    abstract::Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(199604)43:4<484::AID-MRD11

    authors: Pastisson C,Lacorre I

    更新日期:1996-04-01 00:00:00

  • Expression of prepro-GnRH and GnRH receptor messengers in rainbow trout ovary depends on the stage of ovarian follicular development.

    abstract::Gonadotropin-Releasing Hormones (GnRHs) are decapeptides well known to regulate the reproductive cycle. They are expressed not only in the brain, but also in other tissues including the gonads. It is believed that they may be involved in the endocrine and paracrine regulation of the reproductive cycle. To date, two fo...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.10065

    authors: Uzbekova S,Lareyre JJ,Madigou T,Davail B,Jalabert B,Breton B

    更新日期:2002-05-01 00:00:00

  • Age-associated changes in gene expression and developmental competence of bovine oocytes, and a possible countermeasure against age-associated events.

    abstract::In general, maternal age affects the quality of oocytes and embryos. The present study aimed to examine the features and age-associated gene expression profiles of bovine oocytes and embryos as well as to discover possible countermeasures against age-associated events. Comprehensive gene expression assays of germinal ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22187

    authors: Takeo S,Kawahara-Miki R,Goto H,Cao F,Kimura K,Monji Y,Kuwayama T,Iwata H

    更新日期:2013-07-01 00:00:00

  • Growth retardation in rats whose growth hormone gene expression was suppressed by antisense RNA transgene.

    abstract::We produced four transgenic founder rats (F0) by introducing into rat embryos a fusion gene, which consisted of rat growth hormone (GH) promoter containing with four copies of thyroid hormone response element (TRE) and antisense cDNA sequences for rat GH. This transgene promoter directed 2.8-fold stimulation of CAT ge...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080360109

    authors: Matsumoto K,Kakidani H,Takahashi A,Nakagata N,Anzai M,Matsuzaki Y,Takahashi Y,Miyata K,Utsumi K,Iritani A

    更新日期:1993-09-01 00:00:00

  • Analysis and significance of messenger RNA in human ejaculated spermatozoa.

    abstract::Human ejaculate spermatozoa contain multiple mRNA species carried over from earlier stages in spermatogenesis. To date, gene-specific RT-PCR or in situ hybridization has detected transcripts for beta-actin, heat shock proteins (HSP) 70 and 90, protamines (PRM) 1 and 2, transition protein (TNp) 2, HLA II, beta-integrin...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/(SICI)1098-2795(200006)56:2+<259::AID-MRD1

    authors: Miller D

    更新日期:2000-06-01 00:00:00

  • Responsiveness of rabbits to superovulation treatment by a single injection of follicle-stimulating hormone with aluminum hydroxide gel.

    abstract::Aluminum hydroxide gel (Al-gel), which is used as an adjuvant, can absorb macromolecules. We investigated the applicability of Al-gel to the sustained release of follicle-stimulating hormone (FSH) as a simplified method of superovulation (SOV) in rabbits. The responsiveness of rabbits to SOV by a single injection of F...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20672

    authors: Hashimoto S,Kimura K,Kuramochi T,Aoyagi K,Hirako M,Kawaguchi M,Iwata H,Hirao M,Kitada K,Hirasawa K,Ueda M

    更新日期:2007-09-01 00:00:00

  • Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells.

    abstract::Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related ...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20485

    authors: Ma Q,Wang H,Guo R,Wang H,Ge Y,Ma J,Xue S,Han D

    更新日期:2006-09-01 00:00:00

  • The 26S proteasome: ubiquitin-mediated proteolysis in the tunnel.

    abstract::The 26S proteasome is a self-compartmentalizing protease responsible for the degradation of intracellular proteins. This giant intracellular protease is formed by several subunits arranged into two 19S polar caps-where protein recognition and ATP-dependent unfolding occur-flanking a 20S central barrel-shaped structure...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章,评审

    doi:10.1002/1098-2795(200010)57:2<109::AID-MRD1>3.0.CO

    authors: Kierszenbaum AL

    更新日期:2000-10-01 00:00:00

  • Effects of gonadotrophins, growth hormone, and activin A on enzymatically isolated follicle growth, oocyte chromatin organization, and steroid secretion.

    abstract::So far, standard follicle culture systems can produce blastocyst from less than 40% of the in vitro matured oocytes compared to over 70% in the in vivo counterpart. Because the capacity for embryonic development is strictly associated with the terminal stage of oocyte growth, the nuclear maturity status of the in vitr...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20762

    authors: Ola SI,Ai JS,Liu JH,Wang Q,Wang ZB,Chen DY,Sun QY

    更新日期:2008-01-01 00:00:00

  • Molecular characterization and expression of two cyp19 (P450 aromatase) genes in embryos, larvae, and adults of Atlantic halibut (Hippoglossus hippoglossus).

    abstract::P450aromatase (CYP19) is an essential part of the enzyme complex catalyzing the conversion of androgens into estrogens, which is a key reaction in the sex differentiation in vertebrates. Two full-length cDNAs encoding the genetic distinct CYP19A and CYP19B isozymes were isolated from Atlantic halibut (Hippoglossus hip...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.20373

    authors: van Nes S,Moe M,Andersen Ø

    更新日期:2005-12-01 00:00:00

  • Evaluation of heterologous insulator function with regard to chromosomal position effect in the mouse blastocyst and fetus.

    abstract::Insulators are located at the boundaries of differentially regulated genes and delimit their interactions by establishing independent chromatin structures. Recently, an insulator sequence has been found in the 5'-flanking region of arylsulfatase (ARS) gene from sea urchin. To investigate functional conservation of thi...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/1098-2795(200011)57:3<232::AID-MRD4>3.0.CO

    authors: Takada T,Iida K,Akasaka K,Yasue H,Torii R,Tsujimoto G,Taira M,Kimura H

    更新日期:2000-11-01 00:00:00

  • Lipovitellins and phosvitins of the fertilized eggs during embryo growth in the oviparous lizard Podarcis sicula.

    abstract::In the lizard Podarcis sicula, the major vitellogenin (VTG)-derived yolk proteins, lipovitellins and phosvitins, were extracted from the yolk globules of laid and fertilized eggs at different periods of incubation up to 44 days close to hatching. Embryonic development was almost over at this time. Yolk proteins were i...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.90018

    authors: Romano M,Rosanova P,Anteo C,Limatola E

    更新日期:2002-11-01 00:00:00

  • Distribution of RNase MRP RNA during Xenopus laevis oogenesis.

    abstract::RNase MRP is a ribonucleoprotein endoribonuclease found predominantly in nucleoli, but which has been associated with mitochondria and mitochondrial RNA processing. In order to analyze the intracellular localization of specific RNA components of ribonucleoproteins of this type, a whole-mount method for in situ hybridi...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.1080420313

    authors: Davis AF,Jeong-Yu S,Clayton DA

    更新日期:1995-11-01 00:00:00

  • Autoregulation of Musashi1 mRNA translation during Xenopus oocyte maturation.

    abstract::The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

    journal_title:Molecular reproduction and development

    pub_type: 杂志文章

    doi:10.1002/mrd.22060

    authors: Arumugam K,Macnicol MC,Macnicol AM

    更新日期:2012-08-01 00:00:00