Abstract:
:Abnormal oocyte spindle due to the improper function of ooplasm is associated with female infertility of advanced maternal age. A possible way to overcome this problem is to transfer an oocyte germinal vesicle (GV) which contains genetic materials of a patient with a history of poor embryo development to the cytoplast from a donor oocyte. Here we demonstrate that GV transfer is feasible using a rabbit model. When the GVs were transferred to auto- or hetero-cytoplasts of GV stage oocytes, around 80% of the reconstructed oocytes could mature in vitro and 7.1-9.4% of the oocytes developed to blastocyst stage after intracytoplasmic sperm injection (ICSI). Transfer of 93 fertilized eggs reconstructed via GV transfer into six recipients resulted in two live offspring. Results of this experiment indicate that GV transfer can potentially become a new approach in treatment of infertility because of advanced maternal age.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Li GP,Chen DY,Lian L,Sun QY,Wang MK,Liu JL,Li JS,Han ZMdoi
10.1002/1098-2795(200102)58:2<180::AID-MRD7>3.0.COkeywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
180-5issue
2eissn
1040-452Xissn
1098-2795pii
10.1002/1098-2795(200102)58:2<180::AID-MRD7>3.0.COjournal_volume
58pub_type
杂志文章abstract::Factors affecting the efficiency of producing transgenic rats by intracytoplasmic sperm injection (ICSI)-mediated DNA transfer were investigated. Epididymal spermatozoa from Sprague-Dawley (SD) rats were sonicated and/or frozen-thawed for cutting the tail and membrane disruption. The sperm heads were exposed for 1 min...
journal_title:Molecular reproduction and development
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更新日期:2005-04-01 00:00:00
abstract::The present study was undertaken to evaluate the effects of hyperstimulation and aging on the number and proportion of oocytes in the metaphase II stage in female Wistar rats. It explored the validity of the hypothesis that a combination of hyperstimulation with pregnant mare serum gonadotrophins (PMSG) and age could ...
journal_title:Molecular reproduction and development
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doi:10.1002/(SICI)1098-2795(200001)55:1<104::AID-MRD14
更新日期:2000-01-01 00:00:00
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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doi:10.1002/mrd.1080250105
更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/mrd.10124
更新日期:2002-07-01 00:00:00
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doi:10.1002/mrd.1080390410
更新日期:1994-12-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22120
更新日期:2012-11-01 00:00:00
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doi:10.1002/mrd.10208
更新日期:2003-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20683
更新日期:2007-06-01 00:00:00
abstract::Human endometrial epithelium (EE) is composed of a multitude of proteins, amongst which those localized on the plasma membrane [plasma membrane proteins (PMPs)] are of critical relevance in the early stages of implantation. Evidence supports the key role of few PMPs in implantation. However, many remain unidentified, ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.23292
更新日期:2020-01-01 00:00:00
abstract::Binding of neurotrophic ligands to rabbit spermatozoa was studied. Nicotinic cholinergic antagonists, [3H]alpha-bungarotoxin and [3H]dihydro-beta-erythroidine (DE), bound with high affinity to different sites in the tails of rabbit spermatozoa with the former binding to 10,207 sites/cell and the latter to 562 sites/ce...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080280109
更新日期:1991-01-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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更新日期:1996-06-01 00:00:00
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更新日期:2001-08-01 00:00:00
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更新日期:2011-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2017-02-01 00:00:00
abstract::Previously we demonstrated that lines of transgenic mice carrying the herpes simplex type 1 virus thymidine kinase (HSV1-tk) reporter gene are male-sterile. Ectopic transcription of the HSV1-tk reporter in the testis was initiated downstream of the normal translation initiation codon and truncated proteins consistent ...
journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1995-08-01 00:00:00
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更新日期:2004-06-01 00:00:00
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更新日期:2004-04-01 00:00:00
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abstract::Progress in the understanding of early mammalian embryo development has been severely hampered by scarcity of study materials. To circumvent such a constraint, we have developed a strategy that involves a combination of in silico mining of new genes from expressed sequence tags (EST) databases and rapid determination ...
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更新日期:2001-07-01 00:00:00