Factors affecting production of transgenic rats by ICSI-mediated DNA transfer: effects of sonication and freeze-thawing of spermatozoa, rat strains for sperm and oocyte donors, and different constructs of exogenous DNA.

abstract：:The maturation of various aspects of sperm function have been demonstrated in monkey and human epididymal sperm, including the ability to undergo the acrosome reaction. The present study aimed to investigate the maturational changes in non-human primate sperm in the signal transduction mechanisms leading to the acroso...

journal_title：Molecular reproduction and development

authors： Yeung CH,Weinbauer GF,Cooper TG

更新日期：1999-10-01 00:00:00

abstract：:Morphogenesis of the Eledone cirrhosa sperm nucleus, as studied by electron microscopic techniques, is compared with that of Octopus vulgaris. Both species of cephalopods belong to the family Octopodidae. The results indicate that extensive nuclear helicoidization during E. cirrhosa spermiogenesis is brought about by ...

journal_title：Molecular reproduction and development

authors： Gimenez-Bonafé P,Ribes E,Zamora MJ,Kasinsky HE,Chiva M

更新日期：2002-07-01 00:00:00

abstract：:There are always more evidences indicating that 17beta-estradiol (E2) is to be necessary for normal male fertility. Here we report the expression of the most ubiquitously expressed member of the akt family of genes, akt1, in the lizard (Podarcis s. sicula) testis. We have used a nonmammalian vertebrate model (the liza...

journal_title：Molecular reproduction and development

authors： Russo M,Troncone G,Guarino FM,Angelini F,Chieffi P

更新日期：2005-05-01 00:00:00

abstract：:Recent studies have independently shown that cyclic adenosine 3'5'-monophosphate (cAMP) modulation prior to in vitro maturation (IVM) and epidermal growth factor (EGF)-like peptide supplementation during IVM improve subsequent oocyte developmental outcomes. This study investigated the effects of an IVM system that inc...

journal_title：Molecular reproduction and development

authors： Richani D,Wang X,Zeng HT,Smitz J,Thompson JG,Gilchrist RB

更新日期：2014-05-01 00:00:00

abstract：:Artificial activation is required for successful intracytoplasmic sperm injection (ICSI) to induce haploidy pronuclear formation with extraction of second polar body. The present study showed that an additional treatment with Phorbol 12-myristate 13-acetate (PMA) followed by Ca(2+) ionophore treatment improved the rat...

journal_title：Molecular reproduction and development

authors： Ito J,Shimada M

更新日期：2005-01-01 00:00:00

abstract：:This study was conducted to investigate the effect of recipient activation time on the chromatin structure and development of bovine nuclear transfer embryos. Serum-starved skin cells were electrofused to enucleated oocytes, activated 1-5 hr after fusion, and cultured in vitro. Some fused eggs were fixed at each time ...

journal_title：Molecular reproduction and development

authors： Choi JY,Kim CI,Park CK,Yang BK,Cheong HT

更新日期：2004-11-01 00:00:00

abstract：:The analysis of differences in gene expression, responding to cryopreservation may explain some of the observed differences in further development of the preimplantation stage embryos. The aim of this study was to create a link, for the first time, between morphological/developmental observations and gene activity cha...

journal_title：Molecular reproduction and development

authors： Boonkusol D,Gal AB,Bodo S,Gorhony B,Kitiyanant Y,Dinnyes A

更新日期：2006-06-01 00:00:00

abstract：:Although the epididymal environment promotes the maturation and survival of spermatozoa, not all spermatozoa remain viable during passage through the epididymis. Does the epididymis has a protective mechanism(s) to segregate the viable sperm from defective spermatozoa? Previously, we identified 260/280 kDa oligomers (...

journal_title：Molecular reproduction and development

authors： Nagdas SK,Wallace S,Eaford D,Baker R,Carr K,Raychoudhuri SS

更新日期：2020-12-01 00:00:00

abstract：:Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....

journal_title：Molecular reproduction and development

authors： Moorhouse KS,Burgess DR

更新日期：2014-10-01 00:00:00

abstract：:Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ line specification during embryogenesis is conceptually the same as the specification of any som...

journal_title：Molecular reproduction and development

authors： Ewen-Campen B,Schwager EE,Extavour CG

更新日期：2010-01-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Apoptosis is a well-established cellular mechanism for selective cell deletion during development. However, little is known about the expression of an apoptotic pathway and its role in determining the relative sensitivity of the early, pre-gastrula, avian embryo to stress-induced cell death. We examined the sensitivit...

journal_title：Molecular reproduction and development

authors： Muscarella DE,Rachlinski MK,Bloom SE

更新日期：1998-10-01 00:00:00

abstract：:The effects of cryopreservation on the frequency and type of chromosomal abnormalities in human sperm were investigated. Employing a technique that enables direct visualization of human sperm chromosomes following in vitro penetration of hamster oocytes, sperm samples from 10 normal men were examined before and after ...

journal_title：Molecular reproduction and development

authors： Martin RH,Chernos JE,Rademaker AW

更新日期：1991-10-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) hold great promise for cell-based therapies and drug screening applications. However, growing and processing large quantities of undifferentiated hESCs is a challenging task. Conventionally, hESCs are passaged as clusters, which can limit their growth efficiency and use in downstream...

journal_title：Molecular reproduction and development

authors： Bajpai R,Lesperance J,Kim M,Terskikh AV

更新日期：2008-05-01 00:00:00

abstract：:To study possible role and regulation of apoptosis occurred in primate endometrium, the expression of apoptosis-related molecules, Fas, FasL, B cell lymphoma/leukaemia-2 (Bcl-2), and Bax were analyzed in relation to occurrence of apoptosis and proliferation in the cycling endometrium of the rhesus monkey using immunoh...

journal_title：Molecular reproduction and development

authors： Wei P,Jin X,Tao SX,Han CS,Liu YX

更新日期：2005-04-01 00:00:00

abstract：:The aim of this study was to elucidate the relationship between protein tyrosine phosphorylation state and sperm characteristics in frozen-stored spermatozoa of Japanese Black bulls. The spermatozoa were washed with PBS containing polyvinyl alcohol and then incubated with cell-permeable cAMP analog cBiMPS to induce fl...

journal_title：Molecular reproduction and development

authors： Harayama H,Nishijima K,Murase T,Sakase M,Fukushima M

更新日期：2010-10-01 00:00:00

abstract：:Sperm-mediated gene transfer in vertebrates has undergone various developments over the last few years, in different laboratories. In the present study, we microinjected a circular plasmid, carrying the lacZ reporter gene mixed with noncommercial cationic lipids, into the seminiferous tubules of anesthetized adult mic...

journal_title：Molecular reproduction and development

authors： Celebi C,Auvray P,Benvegnu T,Plusquellec D,Jégou B,Guillaudeux T

更新日期：2002-08-01 00:00:00

abstract：:The effect of two glycoproteins (estrus-associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP-1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct-c...

journal_title：Molecular reproduction and development

authors： Vansteenbrugge A,Van Langendonckt A,Massip A,Dessy F

更新日期：1997-04-01 00:00:00

abstract：:Integrins are a family of cell adhesion receptors involved in many cell-cell and cell-matrix interactions. Some of these heterodimeric receptors, such as alpha 5 beta 1 and alpha v beta 1, specifically recognize the amino acid sequence Arg-Gly-Asp (RGD) within their ligands. The RGD sequence is found in fibronectin, v...

journal_title：Molecular reproduction and development

authors： Fusi FM,Vignali M,Gailit J,Bronson RA

更新日期：1993-10-01 00:00:00

abstract：:The cytoplasmic beta-actin promoter, commonly used as strong promoter in many gene regulation studies, produces a pattern of male germ cell and preimplantation, embryonic gene expression in transgenic mice. In seven of ten expressing transgenic lines, a chicken beta-actin-lacZ fusion gene was expressed in adult testes...

journal_title：Molecular reproduction and development

authors： Sands AT,Hansen TN,Demayo FJ,Stanley LA,Xin L,Schwartz RJ

更新日期：1993-02-01 00:00:00

abstract：:The plasmid DNA, pAcZ, containing Escherichia coli beta-galactosidase (lacZ) gene under the control of chicken beta-actin gene promoter was injected in a linearized form into the germinal disc of fertilized chick ova at the single-cell stage. The manipulated embryos were cultured by the method of Naito et al. (1990) u...

journal_title：Molecular reproduction and development

authors： Naito M,Sasaki E,Ohtaki M,Sakurai M

更新日期：1994-02-01 00:00:00

abstract：:We have examined the developmental pattern of prolactin receptor expression in the mouse by reverse transcription-polymerase chain reaction, in situ hybridization, and radioligand binding and have found two unexpected aspects of temporal regulation. First, high levels of prolactin receptor mRNA were detected in mouse ...

journal_title：Molecular reproduction and development

authors： Tzeng SJ,Linzer DI

更新日期：1997-09-01 00:00:00

abstract：:We have investigated the origin of the sperm motility inhibitor (SPMI) from boar seminal plasma. SPMI was measured by its capacity to inhibit the motility of demembranated spermatozoa and by an enzyme-linked immunosorbent assay (ELISA). Among the various reproductive and now reproductive tissues and fluids tested, onl...

journal_title：Molecular reproduction and development

authors： Iwamoto T,Tanaka H,Osada T,Shinagawa T,Osamura Y,Gagnon C

更新日期：1993-12-01 00:00:00

abstract：:Fully grown immature oocytes acquire the ability to be fertilized with sperm after meiotic maturation, which is finally accomplished by the formation and activation of the maturation-promoting factor (MPF). MPF is the complex of Cdc2 and cyclin B, and its function in promoting metaphase is common among species. The Mo...

journal_title：Molecular reproduction and development

authors： Yoshida N,Mita K,Yamashita M

更新日期：2000-09-01 00:00:00

abstract：:Formation and maintenance of testis cords during embryogenesis are essential for establishing testicular structure and function in adults. At least five genes (Wt1, Dhh, Sox8/Sox9, and Dax1) appear to be required for the maintenance of testis cord integrity in mice. Here, we report that RFX1 is specifically expressed ...

journal_title：Molecular reproduction and development

authors： Wang B,Qi T,Chen SQ,Ye L,Huang ZS,Li H

更新日期：2016-07-01 00:00:00

abstract：:Protectin (CD59) is a complement regulatory protein which blocks the membrane attack complex during complement activation. CD59 was identified on the human sperm surface by means of H19, an IgG1 anti-protectin mouse monoclonal antibody. Using indirect immunofluorescence, flow cytometry and immunoperoxidase, CD59 was f...

journal_title：Molecular reproduction and development

authors： Fenichel P,Cervoni F,Hofmann P,Deckert M,Emiliozzi C,Hsi BL,Rossi B

更新日期：1994-07-01 00:00:00

abstract：:During early placental development, tumor suppressors and oncogenes work synergistically to regulate cell proliferation and differentiation in a restrained manner compared with the uncontrollable growth in cancer. One example of this partnership is the regulation of the oncofetal protein HMGA2 by BRCA1. BRCA1 forms a ...

journal_title：Molecular reproduction and development

authors： West RC,Russ JE,Bouma GJ,Winger QA

更新日期：2019-11-01 00:00:00

abstract：:Several animal and human studies have demonstrated that sex affects kinetics and metabolism during early embryo development. However, the mechanism governing these differences at the molecular level before the expression of the sex-determining gene SRY is unknown. We performed a systematic profiling of gene expression...

journal_title：Molecular reproduction and development

authors： Zhou Q,Wang T,Leng L,Zheng W,Huang J,Fang F,Yang L,Chen F,Lin G,Wang WJ,Kristiansen K

更新日期：2019-07-01 00:00:00

abstract：:A novel restriction fragment differential display (RFDD) RT-PCR has been used to compare patterns of mRNA expression in bovine oocytes matured in vitro in the presence (10%) or absence of fetal calf serum (FCS). Total RNA extracted from matured and denuded oocytes was processed using display Profile kit (Display Syste...

journal_title：Molecular reproduction and development

authors： Rzucidlo SJ,Gibbons J,Stice SL

更新日期：2001-05-01 00:00:00

abstract：:Epigenetics includes the study of external factors that can influence the expression of genes by altering the accessibility of DNA through methylation. To investigate the epigenetic influence of season, sperm head shape, and semen storage on placental and fetal tissues, pregnancies were generated in the summer or wint...

journal_title：Molecular reproduction and development

authors： Rempel LA,Krautkramer MM,Parrish JJ,Miles JR

更新日期：2019-04-01 00:00:00