Origin of a sperm motility inhibitor from boar seminal plasma.

abstract：:The text below is a transcript of the keynote address Kenneth Manning gave at the symposium honoring E. E. Just that was held on the campus of Howard University on November 21, 2008. In his talk, Manning reflects on his experiences researching and writing Black Apollo of Science: The Life of Ernest Everett Just, his p...

journal_title：Molecular reproduction and development

authors： Manning KR

更新日期：2009-10-01 00:00:00

abstract：:Diandric and digynic triploid mouse embryos were isolated in the morning on day 10 of gestation. The embryos were separated from their extraembryonic membranes, and the latter were analysed cytogenetically by G-banding to establish the ploidy and sex chromosome constitution of these embryos. The diandric triploid embr...

journal_title：Molecular reproduction and development

authors： Kaufman MH,Lee KK,Speirs S

更新日期：1990-04-01 00:00:00

abstract：:The present study was conducted to investigate the mechanisms underlying fluid movement across the trophectoderm during blastocyst formation by determining whether aquaporins (AQPs) are expressed during early mammalian development. AQPs belong to a family of major intrinsic membrane proteins and function as molecular ...

journal_title：Molecular reproduction and development

authors： Offenberg H,Barcroft LC,Caveney A,Viuff D,Thomsen PD,Watson AJ

更新日期：2000-12-01 00:00:00

abstract：:Nuclear import of plasmid DNA mediated by a nuclear localization signal (NLS) derived from SV40 T antigen was investigated in a cell-free extract. In vitro assembled sea urchin male pronuclei were incubated in a 100,000g supernatant of a zebrafish fertilized egg lysate, together with fluorescently labeled plasmid DNA ...

journal_title：Molecular reproduction and development

authors： Collas P,Aleström P

更新日期：1996-12-01 00:00:00

abstract：:A novel gene Ggnbp1 was identified during yeast two-hybrid screening of gametogenetin protein 1 (GGN1)-interacting proteins. Ggnbp1 gene was found in mouse, rat, and human genomes but not in sequenced yeast, worms, fly, or fish genomes. Northern blotting analysis revealed that the gene was specifically expressed in th...

journal_title：Molecular reproduction and development

authors： Zhou Y,Zhao Q,Bishop CE,Huang P,Lu B

更新日期：2005-03-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00

abstract：:Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and ...

journal_title：Molecular reproduction and development

authors： Garside WT,Loret de Mola JR,Bucci JA,Tureck RW,Heyner S

更新日期：1997-05-01 00:00:00

abstract：:The aim of the present study was to investigate the mechanism of androgen-mediated, prolonged sperm-storage in the female reproductive tract of the bat, Scotophilus heathii. The bat treated in vivo with flutamide, an androgen antagonist, showed loss of spermatozoa at the storage site, utero-tubal junction. Immunocytoc...

journal_title：Molecular reproduction and development

authors： Roy VK,Krishna A

更新日期：2011-07-01 00:00:00

abstract：:The purpose of the present study was to investigate the effects of prostaglandin (PG) E(2) and tumor necrosis factor (TNF) alpha on expression of vascular endothelial growth factor (VEGF) and its receptors, fms-like tyrosine kinase (Flt-1) and fetal liver kinase-1/kinase insert domain-containing receptor (Flk-1/KDR), ...

journal_title：Molecular reproduction and development

authors： Kowalczyk AE,Kaczmarek MM,Schams D,Ziecik AJ

更新日期：2008-10-01 00:00:00

abstract：:Estrogen receptor (ER)- (alpha, beta, and both alpha and beta) and aromatase (Ar) knockout (KO) mice have been created to assess the biological effects of estrogens. This review article discusses the ovarian and uterine phenotypes of these mice. The data obtained have confirmed some older inferences about how the ster...

journal_title：Molecular reproduction and development

authors： Rosenfeld CS,Roberts RM,Lubahn DB

更新日期：2001-07-01 00:00:00

abstract：:In this study, we document that the overall rate of protein synthesis decreases during in vitro maturation (IVM) of pig oocytes despite enhanced formation of the 5' cap structure eIF4F. Within somatic/interphase cells, formation of the eIF4F protein complex correlates very well with overall rates of protein translatio...

journal_title：Molecular reproduction and development

authors： Ellederova Z,Kovarova H,Melo-Sterza F,Livingstone M,Tomek W,Kubelka M

更新日期：2006-01-01 00:00:00

abstract：:We aimed in this study to explore if sphingosine-1-phosphate (S1P) reduces apoptosis of primordial follicles during cryopreservation of human ovarian cortical samples. Ovarian cortical tissue fragments obtained from young patients who underwent laparoscopic excision of benign ovarian cysts were used for the experiment...

journal_title：Molecular reproduction and development

authors： Guzel Y,Bildik G,Dilege E,Oktem O

更新日期：2018-11-01 00:00:00

abstract：:An early step in mammalian fertilization is species-restricted binding of sperm to the egg's zona pellucida (ZP), a thick extracellular coat that surrounds eggs. Sperm bind to the ZP of unfertilized eggs, but not to the ZP of fertilized eggs. Shortly after binding to the unfertilized egg ZP, sperm undergo the acrosome...

journal_title：Molecular reproduction and development

authors： Litscher ES,Williams Z,Wassarman PM

更新日期：2009-10-01 00:00:00

abstract：:cDNA clones encoding stage-and/or space-specific genes in porcine ovarian follicles have been investigated by differential screening of cDNA libraries of granulosa cells and corpus luteum. A clone corresponding to mRNA preferentially expressed in the functional stage of corpus luteum was found to encode a protein simi...

journal_title：Molecular reproduction and development

authors： Tanaka T,Itahana K,Andoh N,Takeya T,Sato E

更新日期：1995-10-01 00:00:00

abstract：:Activation of bovine oocytes by experimental procedures that closely mimic normal fertilization and allow to obtain haploid oocytes is essential both for intracytoplasmic sperm injection (ICSI) and for nuclear transfer. Therefore, with the goal of producing haploid activated oocytes, this study evaluated whether bohem...

journal_title：Molecular reproduction and development

authors： Alberio R,Kubelka M,Zakhartchenko V,Hajdúch M,Wolf E,Motlik J

更新日期：2000-04-01 00:00:00

abstract：:In vitro oocyte maturation followed by in vitro fertilization (IVM/IVF) success in the domestic cat remains inferior to commonly studied livestock or laboratory species. The objectives here were (1) to histologically assess atresia status of freshly excised follicle/oocyte complexes, and (2) to evaluate taphonomic cha...

journal_title：Molecular reproduction and development

authors： Wood TC,Montali RJ,Wildt DE

更新日期：1997-02-01 00:00:00

abstract：:It was reported that a hamster protein, called "oscillin," with a sequence related to that of an Escherichia coli GNPDA triggered Ca(2+) oscillations in mammalian oocytes when introduced into their cytoplasm upon fertilization. Recently, it was shown that GNPDA/oscillin is ubiquitously expressed in rat tissues and tha...

journal_title：Molecular reproduction and development

authors： Amireault P,Dubé F

更新日期：2000-07-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:In the equine, the zona pellucida (ZP) is the major barrier to successful in vitro fertilization. Therefore the aim of our studies was to analyze species-specific features of the equine ZP in regard to structure and glycoprotein ZPB and ZPC expression sites during oocyte development and embryogenesis. The equine ZP re...

journal_title：Molecular reproduction and development

authors： Kölle S,Dubois CS,Caillaud M,Lahuec C,Sinowatz F,Goudet G

更新日期：2007-07-01 00:00:00

abstract：:Although placental transfer of maternal calcium (Ca(2+)) is a crucial process for fetal development, the biochemical mechanisms are not completely elucidated. Especially, mechanisms of syncytiotrophoblast Ca(2+) extrusion into fetal circulation remain to be established. In the current study we have investigated the ch...

journal_title：Molecular reproduction and development

authors： Moreau R,Daoud G,Masse A,Simoneau L,Lafond J

更新日期：2003-07-01 00:00:00

abstract：:Various thiol compounds are known to improve cytoplasmic and/or nuclear maturation of oocytes in vitro. The present study examined the effects of two thiol compounds, cysteine (0.1, 0.5, and 1.0 mM) and cysteamine (50, 100, and 200 microM), on cytoplasmic and nuclear maturation of canine oocytes. Oocytes collected fro...

journal_title：Molecular reproduction and development

authors： Hossein MS,Kim MK,Jang G,Oh HJ,Koo O,Kim JJ,Kang SK,Lee BC,Hwang WS

更新日期：2007-09-01 00:00:00

abstract：:Oocytes enucleated at metaphase II stage can support reprogramming of transferred nucleus and further developing to term. However, the first polar body in mice sometimes migrates away from the original place of expulsion, so the chromosomes of the oocyte will displace from the first polar body. Thus, it is not always ...

journal_title：Molecular reproduction and development

authors： Wang MK,Liu JL,Li GP,Lian L,Chen DY

更新日期：2001-04-01 00:00:00

abstract：:We determined the incidence of activation, male pronuclear formation, and apposition of pronuclei in porcine oocytes following intracytoplasmic injection of various porcine sperm components and foreign species spermatozoa, such as that of cattle, mouse or human. The porcine oocytes were activated by injection of a spe...

journal_title：Molecular reproduction and development

authors： Kim NH,Jun SH,Do JT,Uhm SJ,Lee HT,Chung KS

更新日期：1999-05-01 00:00:00

abstract：:The production of chimeras, by use of cell transplantation, has proved to be highly valuable in studies of development by providing insights into cell fate, differentiation, and developmental potential. So far, chimeric honeybees have been created by nuclear transfer technologies. We have developed protocols to produc...

journal_title：Molecular reproduction and development

authors： Bergem M,Norberg K,Roseth A,Meuwissen T,Lien S,Aamodt RH

更新日期：2006-04-01 00:00:00

abstract：:The vascular cell responses to the type 1, 2, and 3 isoforms of transforming growth factor-beta (TGF-beta 1, TGF-beta 2, TGF-beta 3) were studied using bovine aortic endothelial (BAECs) and smooth muscle cells (BASMC3) as well as rat epididymal fat pad microvascular endothelia (RFCs). Three distinct bioassays indicate...

journal_title：Molecular reproduction and development

authors： Madri JA,Bell L,Merwin JR

更新日期：1992-06-01 00:00:00

abstract：:Gamete membrane interaction is critical to initiate the development of a new organism. The signaling pathways governing this event, however, are poorly understood. In this report, we provide the first evidence that protein kinase C activity in mouse eggs plays a crucial role in the regulation of this process. Stimulat...

journal_title：Molecular reproduction and development

authors： Akabane H,Fan J,Zheng X,Zhu GZ

更新日期：2007-11-01 00:00:00

abstract：:It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (Gt...

journal_title：Molecular reproduction and development

authors： Hu X,Liu X,Zhang H,Zhang Y,Li S,Sang Q,Wang Q,Luo W,Liu Q,Lu D,Meng Z,Lin H

更新日期：2011-06-01 00:00:00

abstract：:This study was performed to investigate the effects of edaravone on hypoxia-induced trophoblast cell proliferation, apoptosis, and invasion. The trophoblast cell line HTR-8 (H8) was treated with cobalt chloride (CoCl2 ) with or without a 1-hr edaravone pretreatment, followed by assessment of intracellular reactive oxy...

journal_title：Molecular reproduction and development

authors： Zheng Y,Zhao Y,Luo Q,Liu X,Liu X,Hu Y,Zou L

更新日期：2016-07-01 00:00:00

abstract：:The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization. Oocytes were aspirated from 2-5 mm follicles of ovaries collected at a local abattoir. The oocyte-cumulus complexes (OCCs) were cultur...

journal_title：Molecular reproduction and development

authors： Rose TA,Bavister BD

更新日期：1992-01-01 00:00:00

abstract：:In this study cytoskeletal antigens common to brushtail possum and tammar wallaby spermatozoa were characterised using a monoclonal antibody (PSA-10). Using indirect immunofluorescence, the PSA-10 antibody detected antigens predominantly associated with the midpiece and principal piece of mature, permeabilised marsupi...

journal_title：Molecular reproduction and development

authors： Harris MS,Rodger JC

更新日期：1998-08-01 00:00:00