Suppression of translation during in vitro maturation of pig oocytes despite enhanced formation of cap-binding protein complex eIF4F and 4E-BP1 hyperphosphorylation.

abstract：:Zona pellucida thickness was measured daily in zygotes and cleavage stage embryos. Measurements were performed on a Nikon inverted microscope equipped with Hoffman modulation optics, using an ocular micrometer. Zona thickness of each zygote/embryo was measured four times, the zygote/embryo was then "rolled over," and ...

journal_title：Molecular reproduction and development

authors： Garside WT,Loret de Mola JR,Bucci JA,Tureck RW,Heyner S

更新日期：1997-05-01 00:00:00

abstract：:Implantation is a complex process that requires the interaction of the blastocyst, and subsequently, that of the developing embryos with the endometrium. Several growth factors and cytokines are involved in implantation, but the details of their actions as related to the regulation of blastocyst implantation remain un...

journal_title：Molecular reproduction and development

authors： Taniguchi F,Harada T,Yoshida S,Iwabe T,Onohara Y,Tanikawa M,Terakawa N

更新日期：1998-05-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) belong to the transforming growth factor-beta (TGFβ) superfamily. BMPs mediate a highly conserved signal transduction cascade through the type-I and type-II serine/threonine kinase receptors and intracellular Smad proteins, which regulate multiple developmental and homeostatic proces...

journal_title：Molecular reproduction and development

authors： Tian C,Liu J

更新日期：2013-09-01 00:00:00

abstract：:The present study aims at quantification of gonadotropin releasing hormone (GnRH) by radioimmunoassay, relative expression of its mRNA by real-time PCR accompanied by its cellular localization in the rat ovary by immunonohistochemistry (IHC) during different time points of pregnancy. To determine the involvement of en...

journal_title：Molecular reproduction and development

authors： Sengupta A,Chakrabarti N,Sridaran R

更新日期：2008-06-01 00:00:00

abstract：:Among Sicilian stick insects, two hybridogenetic complexes have been discovered: Bacillus rossius-grandii benazzii and B. rossius-grandii grandii, which also produce androgenetic offspring. The egg maturation of the former is analyzed here through DAPI fluorometry, which, besides the assessment of the meiotic stages, ...

journal_title：Molecular reproduction and development

authors： Tinti F,Scali V

更新日期：1992-11-01 00:00:00

abstract：:Embryonal carcinoma (EC) cells are used widely as a model system for studying the expression of developmentally regulated genes, in particular genes that are regulated at the transcriptional level when EC cells differentiate. This review focuses on the molecular mechanisms that govern the transcription of the fibrobla...

journal_title：Molecular reproduction and development

authors： Lamb KA,Rizzino A

更新日期：1998-10-01 00:00:00

abstract：:The mRNA translational control protein, Musashi, plays a critical role in cell fate determination through sequence-specific interactions with select target mRNAs. In proliferating stem cells, Musashi exerts repression of target mRNAs to promote cell cycle progression. During stem cell differentiation, Musashi target m...

journal_title：Molecular reproduction and development

authors： Arumugam K,Macnicol MC,Macnicol AM

更新日期：2012-08-01 00:00:00

abstract：:Proteins in the seminal fluid of animals with internal fertilization effect numerous responses in mated females that impact both male and female fertility. Among these proteins is the highly represented class of proteolysis regulators (proteases and their inhibitors). Though proteolysis regulators have now been identi...

journal_title：Molecular reproduction and development

authors： Laflamme BA,Wolfner MF

更新日期：2013-02-01 00:00:00

abstract：:Spermiogenesis pursues three major objectives: (1) The safeguard of the male genome within the confines of a compact nucleus. (2) The accumulation of enzymes in the acrosome of be released at fertilization. (3) The development of a sperm propelling tail consisting of an axoneme surrounded by a scaffold of keratin-cont...

journal_title：Molecular reproduction and development

authors： Kierszenbaum AL

更新日期：2001-08-01 00:00:00

abstract：:The effect of two glycoproteins (estrus-associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP-1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct-c...

journal_title：Molecular reproduction and development

authors： Vansteenbrugge A,Van Langendonckt A,Massip A,Dessy F

更新日期：1997-04-01 00:00:00

abstract：:The first indication that the insulin-like growth factor-II/mannose 6-phosphate receptor (IGF-II/M6PR) is developmentally regulated came from studies of the serum form of the receptor in the rat. By immunoblotting, the circulating form of the receptor, which was 10 kDa smaller than the tissue receptor, was high in 19 ...

journal_title：Molecular reproduction and development

authors： Nissley P,Kiess W,Sklar M

更新日期：1993-08-01 00:00:00

abstract：:Protectin (CD59) is a complement regulatory protein which blocks the membrane attack complex during complement activation. CD59 was identified on the human sperm surface by means of H19, an IgG1 anti-protectin mouse monoclonal antibody. Using indirect immunofluorescence, flow cytometry and immunoperoxidase, CD59 was f...

journal_title：Molecular reproduction and development

authors： Fenichel P,Cervoni F,Hofmann P,Deckert M,Emiliozzi C,Hsi BL,Rossi B

更新日期：1994-07-01 00:00:00

abstract：:Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF...

journal_title：Molecular reproduction and development

authors： Aguero T,Jin Z,Owens D,Malhotra A,Newman K,Yang J,King ML

更新日期：2018-12-01 00:00:00

abstract：:Spindle positioning is an imperative cellular process that regulates a number of different developmental events throughout embryogenesis. The spindle must be properly positioned in embryos not only for the segregation of chromosomes, but also to segregate developmental determinants into different daughter blastomeres....

journal_title：Molecular reproduction and development

authors： Moorhouse KS,Burgess DR

更新日期：2014-10-01 00:00:00

abstract：:Fertilization in Bufo arenarum requires the sperm to penetrate the egg envelopes. The incubation of isolated vitelline envelopes with sperm induces the acrosome reaction, releasing proteases and glycosidases to the media. In the present work N-acetyl-beta-D-glucosaminidase, beta-D-galactosidase, beta-D-glucosidase, al...

journal_title：Molecular reproduction and development

authors： Martínez ML,Martelotto L,Cabada MO

更新日期：2000-10-01 00:00:00

abstract：:In pigs, the morphology and cell number of in vitro-produced blastocysts are inferior to those of their in vivo counterparts. The objective of this study was to increase developmental competence and to gain an understanding of cell allocation in blastocysts derived from the aggregation of four-cell stage porcine embry...

journal_title：Molecular reproduction and development

authors： Lee SG,Park CH,Choi DH,Kim HS,Ka HH,Lee CK

更新日期：2007-11-01 00:00:00

abstract：:Oocyte maturation is an important process required to achieve optimal oocyte quality, and later affects fertilization potential and subsequent embryo development. The maturation process includes synchronized nuclear and cytoplasmic remodeling, in which cytoskeletal and centrosome dynamics play an important role and si...

journal_title：Molecular reproduction and development

authors： Schatten H,Sun QY

更新日期：2011-10-01 00:00:00

abstract：:It is known that the hypothalamic-pituitary-gonadal axis participates in the sex change of hermaphrodite teleosts, and gonadal steroid hormones mediate this physiological process. The secretion of gonadal steroids is directly regulated by signaling pathways involving gonadotropins (GtHs) and gonadotropin receptors (Gt...

journal_title：Molecular reproduction and development

authors： Hu X,Liu X,Zhang H,Zhang Y,Li S,Sang Q,Wang Q,Luo W,Liu Q,Lu D,Meng Z,Lin H

更新日期：2011-06-01 00:00:00

abstract：:Primordial germ cells (PGCs) are useful for producing transgenic chickens and preserving genetic material in avian species. In this study, we investigated the in vitro differentiation potential of chicken PGCs into different cell types. For differentiation into adipocytes, chicken PGCs were cultured for 21 days in ind...

journal_title：Molecular reproduction and development

authors： Li BC,Tian ZQ,Sun M,Xu Q,Wang XY,Qin YR,Xu F,Gao B,Wang KH,Sun HC,Chen GH

更新日期：2010-09-01 00:00:00

abstract：:Germ cells occupy a unique position in animal reproduction, development, and evolution. In sexually reproducing animals, only they can produce gametes and contribute genetically to subsequent generations. Nonetheless, germ line specification during embryogenesis is conceptually the same as the specification of any som...

journal_title：Molecular reproduction and development

authors： Ewen-Campen B,Schwager EE,Extavour CG

更新日期：2010-01-01 00:00:00

abstract：:The dysregulation of microRNAs (miRNAs) plays an important role in asthenozoospermia. This study evaluated the sperm microRNA-423-5p (miR-423-5p) expression in asthenozoospermia and normozoospermia, exploring the role of miR-423-5p in asthenozoospermia. Eighty participants were divided into asthenozoospermic (AZS, n =...

journal_title：Molecular reproduction and development

authors： Zhang R,Zuo Y,Cao S

更新日期：2021-01-28 00:00:00

abstract：:The maturation of various aspects of sperm function have been demonstrated in monkey and human epididymal sperm, including the ability to undergo the acrosome reaction. The present study aimed to investigate the maturational changes in non-human primate sperm in the signal transduction mechanisms leading to the acroso...

journal_title：Molecular reproduction and development

authors： Yeung CH,Weinbauer GF,Cooper TG

更新日期：1999-10-01 00:00:00

abstract：:Changes in spermatozoan ultrastructure have been studied during spermiogenesis of the slug Arion rufus (Gastropoda, Pulmonata, Stylommatophora). The ovotestis was investigated during the male stage, definite by the presence of spermatozoa. Some peculiar characteristics are shown by early spermatids: Around the nucleus...

journal_title：Molecular reproduction and development

authors： Pastisson C,Lacorre I

更新日期：1996-04-01 00:00:00

abstract：:In this study, we sought to determine the mechanism of early sex reversal in a teleost by applying 4 hr feminization treatments to XY (17α-ethynylestradiol 2000 μg L(-1) ) and YY (6500 μg L(-1) ) Nile tilapia embryos on the first day post-fertilization (dpf). We then searched for changes in the expression profiles of ...

journal_title：Molecular reproduction and development

authors： Gennotte V,Mélard C,D'Cotta H,Baroiller JF,Rougeot C

更新日期：2014-12-01 00:00:00

abstract：:Eggs of Ascidia ceratodes and Phallusia mammillata block polyspermy by releasing a phosphatidylinositol-linked glycosidase from the follicle cell and egg surface that binds to and blocks all unoccupied sperm binding sites on the vitelline coat. Release of this glycosidase is thought to be under the control of a membra...

journal_title：Molecular reproduction and development

authors： Robert LK,Lucio-Gough LM,Goode CA,Mckinney K,Lambert CC

更新日期：1999-09-01 00:00:00

abstract：:Plac1 is a recently identified, X-linked gene whose expression is restricted primarily to cells of the trophoblast lineage. It localizes to a chromosomal locus previously implicated in placental growth. We therefore sought to determine if Plac1 is necessary for placental and embryonic development by examining a mutant...

journal_title：Molecular reproduction and development

authors： Jackman SM,Kong X,Fant ME

更新日期：2012-08-01 00:00:00

abstract：:Many methods for efficient production of transgenic animals for biomedical research have been developed. Despite great improvements in transgenesis rates resulting from the use of intracytoplasmic sperm injection (ICSI), the ICSI-based sperm-mediated gene-transfer (iSMGT) technique is still not optimal in terms of spe...

journal_title：Molecular reproduction and development

authors： Sim BW,Cha JJ,Song BS,Kim JS,Yoon SB,Choi SA,Jeong KJ,Kim YH,Huh JW,Lee SR,Kim SH,Lee CS,Kim SU,Chang KT

更新日期：2013-03-01 00:00:00

abstract：:The localization of fibrillarin and nucleolin in the nuclei of mouse two-cell, four-cell, and eight-cell embryos has been studied using immunofluorescent staining with specific antibodies. In all of these cleavage stages, both antigens were associated exclusively with the peripheral region of the nucleolus precursor b...

journal_title：Molecular reproduction and development

authors： Baran V,Veselá J,Rehák P,Koppel J,Fléchon JE

更新日期：1995-03-01 00:00:00

abstract：:Irregular uterine bleeding is a major side effect of long-acting progestogen-only contraceptives in women, and is the primary reason women discontinue their use. In this study, a mouse model of endometrial breakdown was established using a subcutaneous progesterone implant to understand how irregular bleeding begins. ...

journal_title：Molecular reproduction and development

authors： Zhang GH,Cui LJ,Li AY,Zhang JP,Liu Y,Zhao JS,Xu XB,He B,Wang JD,Chu L,Li YF

更新日期：2016-09-01 00:00:00

abstract：:Mitogen-activated protein kinase (MAPK) plays a crucial role in meiotic maturation of mouse oocytes. In order to understand the mechanism by which MAPK regulates meiotic maturation, we examined the effects of the MAPK pathway inhibitor U0126 on microtubule organization, gamma-tubulin and nuclear mitotic apparatus prot...

journal_title：Molecular reproduction and development

authors： Lee SE,Kim JH,Kim NH

更新日期：2007-07-01 00:00:00