Abstract:
:The effect of two glycoproteins (estrus-associated glycoprotein [EGP] and tissue inhibitor of metalloproteinase [TIMP-1]) secreted by bovine oviduct cells on in vitro bovine embryo development was assessed. A first set of experiments was conducted to determine whether the embryotrophic activity of the bovine oviduct-conditioned medium (BOCM) was correlated with the presence of EGP or TIMP-1. EGP and TIMP-1 were detected in BOCM, supporting the development of 22% zygotes to the blastocyst stage, as well as in BOCM yielding a low blastocyst rate (3-4% blastocysts). These glycoproteins do not seem to be necessary for bovine embryo development up to the blastocyst stage in our BOCM. In a second experiment, zygotes were cultured in modified synthetic oviduct fluid (mSOF) supplemented with different concentrations (0.5, 5, 50, and 500 micrograms/ml) of purified bovine EGP. In the third experiment, since purified bovine TIMP-1 was not available, zygotes were cultured in BOCM depleted of TIMP-1 by immunoprecipitation treatment. Adding EGP to mSOF, or removing TIMP-1 from BOCM, did not affect bovine embryo development up to the blastocyst stage, or mean number of cells per blastocyst after 8 days of culture. The results indicate that, under our culture conditions, EGP and TIMP-1 do not play an important role in sustaining bovine embryo development, and do not influence blastocyst quality, assessed in terms of total number of cells per embryo.
journal_name
Mol Reprod Devjournal_title
Molecular reproduction and developmentauthors
Vansteenbrugge A,Van Langendonckt A,Massip A,Dessy Fdoi
10.1002/(SICI)1098-2795(199704)46:4<527::AID-MRD11subject
Has Abstractpub_date
1997-04-01 00:00:00pages
527-34issue
4eissn
1040-452Xissn
1098-2795pii
10.1002/(SICI)1098-2795(199704)46:4<527::AID-MRD11journal_volume
46pub_type
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1996-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1991-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2000-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20048
更新日期:2004-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20792
更新日期:2008-05-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.1080370208
更新日期:1994-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.20512
更新日期:2006-08-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22808
更新日期:2017-06-01 00:00:00
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journal_title:Molecular reproduction and development
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pub_type: 评论,杂志文章
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pub_type: 杂志文章
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更新日期:2000-04-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章,评审
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更新日期:2011-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199610)45:2<139::AID-MRD5>
更新日期:1996-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
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更新日期:1995-02-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/mrd.22802
更新日期:2017-06-01 00:00:00
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journal_title:Molecular reproduction and development
pub_type: 杂志文章
doi:10.1002/(SICI)1098-2795(199711)48:3<301::AID-MRD1>
更新日期:1997-11-01 00:00:00